Detailed information of effector
Summary
| Effector ID | EFF00434  |
| Effector type | Tge |
| Cognate immunity protein | IMU00074 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (178 a.a.); Nucleotide sequence (534 bp) |
| Description | Tge2; Muramidase |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_3037 (Tge2) |
| Coordinates (Strand) | 3502760..3503293 (-) |
| NCBI ID | 70730402 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054D6E4 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Glucosaminidase | PF01832.22 | 6.5e-06 | 49..120 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-177 | VGPGLLYNHAAKTAVNGIDRSTEGTNVSARLCNNISAVTFVSKYKAVCQPIADQLDLPVENLLGLAAQESQYGTGRIARELNNYFSMHAPAPLQIGAEAPLGNASIKVAKFDSFQKSAQSFASSFGTAVRGQRDPMAFAQALVRSGYNTGNAKTGGRDGFARYLADIIIAVRGRMAC |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9449 | - | - |
Protein sequence: 178 a.a. .
>EFF00434 NC_004129:c3503293-3502760 Type VI Glycoside hydrolase Effector (Tge) [Pseudomonas protegens Pf-5]
VGPGLLYNHAAKTAVNGIDRSTEGTNVSARLCNNISAVTFVSKYKAVCQPIADQLDLPVENLLGLAAQESQYGTGRIARE
LNNYFSMHAPAPLQIGAEAPLGNASIKVAKFDSFQKSAQSFASSFGTAVRGQRDPMAFAQALVRSGYNTGNAKTGGRDGF
ARYLADIIIAVRGRMAC*
VGPGLLYNHAAKTAVNGIDRSTEGTNVSARLCNNISAVTFVSKYKAVCQPIADQLDLPVENLLGLAAQESQYGTGRIARE
LNNYFSMHAPAPLQIGAEAPLGNASIKVAKFDSFQKSAQSFASSFGTAVRGQRDPMAFAQALVRSGYNTGNAKTGGRDGF
ARYLADIIIAVRGRMAC*
Nucleotide sequence: 534 bp .
>EFF00434 NC_004129:c3503293-3502760 Type VI Glycoside hydrolase Effector (Tge) [Pseudomonas protegens Pf-5]
GTGGGCCCCGGGTTGCTTTATAATCACGCGGCGAAAACCGCAGTAAATGGAATTGACCGGTCAACCGAGGGAACAAACGT
GAGCGCCAGACTCTGCAACAACATCAGTGCCGTGACCTTTGTCAGCAAGTACAAGGCGGTGTGCCAGCCGATTGCCGACC
AGCTGGACCTGCCGGTGGAAAACCTGCTGGGCCTGGCCGCCCAGGAAAGCCAGTACGGCACCGGGCGCATTGCCCGGGAA
CTGAACAACTACTTTTCCATGCACGCACCGGCCCCCCTGCAGATCGGCGCCGAGGCGCCCCTGGGCAACGCCAGCATCAA
GGTGGCCAAGTTCGACTCCTTCCAGAAGTCCGCCCAGTCCTTTGCTTCGAGCTTTGGCACGGCAGTACGTGGCCAGCGTG
ACCCCATGGCATTCGCCCAGGCGCTGGTACGCAGCGGCTACAACACCGGCAACGCCAAGACCGGCGGCCGGGATGGCTTC
GCCCGCTACCTGGCGGACATCATCATCGCGGTCAGAGGGCGCATGGCGTGCTGA
GTGGGCCCCGGGTTGCTTTATAATCACGCGGCGAAAACCGCAGTAAATGGAATTGACCGGTCAACCGAGGGAACAAACGT
GAGCGCCAGACTCTGCAACAACATCAGTGCCGTGACCTTTGTCAGCAAGTACAAGGCGGTGTGCCAGCCGATTGCCGACC
AGCTGGACCTGCCGGTGGAAAACCTGCTGGGCCTGGCCGCCCAGGAAAGCCAGTACGGCACCGGGCGCATTGCCCGGGAA
CTGAACAACTACTTTTCCATGCACGCACCGGCCCCCCTGCAGATCGGCGCCGAGGCGCCCCTGGGCAACGCCAGCATCAA
GGTGGCCAAGTTCGACTCCTTCCAGAAGTCCGCCCAGTCCTTTGCTTCGAGCTTTGGCACGGCAGTACGTGGCCAGCGTG
ACCCCATGGCATTCGCCCAGGCGCTGGTACGCAGCGGCTACAACACCGGCAACGCCAAGACCGGCGGCCGGGATGGCTTC
GCCCGCTACCTGGCGGACATCATCATCGCGGTCAGAGGGCGCATGGCGTGCTGA