Detailed information of immunity protein
Summary
| Immunity protein ID | IMU00073  |
| Cognate effector | EFF00045 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (150 a.a.); Nucleotide sequence (450 bp) |
| Description | Tai3 |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_5499 (Tai3) |
| Coordinates (Strand) | 6278755..6279204 (+) |
| NCBI ID | 70732800 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054E04E |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF3828 | PF12883.9 | 8e-21 | 27..142 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-149 | MRMLFCSLLAAVAMHQSVALADCASSPKPATQAFYSWYLQTFSEDKDPLTDNVAEMKKYVSDSLITKIKKQMASPDGLEEDYFLKAQDYMDEWLTQIKVSEPQVQGSSAKEQVTLGNTPDTTQIVDVRMIKDKGCWKIDEVISTADQSD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.9893 | pos: 21-22. ALA-DC. | MRMLFCSLLAAVAMHQSVALA |
Protein sequence: 150 a.a. .
>IMU00073 NC_004129:6278755-6279204 [Pseudomonas protegens Pf-5]
MRMLFCSLLAAVAMHQSVALADCASSPKPATQAFYSWYLQTFSEDKDPLTDNVAEMKKYVSDSLITKIKKQMASPDGLEE
DYFLKAQDYMDEWLTQIKVSEPQVQGSSAKEQVTLGNTPDTTQIVDVRMIKDKGCWKIDEVISTADQSD*
MRMLFCSLLAAVAMHQSVALADCASSPKPATQAFYSWYLQTFSEDKDPLTDNVAEMKKYVSDSLITKIKKQMASPDGLEE
DYFLKAQDYMDEWLTQIKVSEPQVQGSSAKEQVTLGNTPDTTQIVDVRMIKDKGCWKIDEVISTADQSD*
Nucleotide sequence: 450 bp .
>IMU00073 NC_004129:6278755-6279204 [Pseudomonas protegens Pf-5]
ATGAGAATGCTGTTCTGCTCGCTGCTGGCAGCGGTCGCGATGCATCAGTCGGTCGCCCTGGCCGACTGCGCCAGTTCGCC
AAAGCCCGCAACCCAGGCGTTTTATAGCTGGTACCTGCAAACCTTCAGCGAAGACAAGGATCCGTTGACCGACAACGTGG
CGGAAATGAAGAAGTACGTCTCCGACTCGCTGATCACCAAGATCAAGAAACAGATGGCCAGCCCCGACGGCCTGGAAGAG
GACTACTTCCTCAAGGCCCAGGACTACATGGATGAGTGGCTGACCCAGATCAAGGTCAGCGAACCACAAGTCCAGGGCTC
CAGCGCCAAGGAGCAAGTGACGTTGGGCAACACCCCGGACACCACCCAGATCGTCGATGTGCGGATGATCAAGGACAAGG
GCTGCTGGAAGATCGATGAAGTGATTTCCACTGCCGACCAGAGCGATTGA
ATGAGAATGCTGTTCTGCTCGCTGCTGGCAGCGGTCGCGATGCATCAGTCGGTCGCCCTGGCCGACTGCGCCAGTTCGCC
AAAGCCCGCAACCCAGGCGTTTTATAGCTGGTACCTGCAAACCTTCAGCGAAGACAAGGATCCGTTGACCGACAACGTGG
CGGAAATGAAGAAGTACGTCTCCGACTCGCTGATCACCAAGATCAAGAAACAGATGGCCAGCCCCGACGGCCTGGAAGAG
GACTACTTCCTCAAGGCCCAGGACTACATGGATGAGTGGCTGACCCAGATCAAGGTCAGCGAACCACAAGTCCAGGGCTC
CAGCGCCAAGGAGCAAGTGACGTTGGGCAACACCCCGGACACCACCCAGATCGTCGATGTGCGGATGATCAAGGACAAGG
GCTGCTGGAAGATCGATGAAGTGATTTCCACTGCCGACCAGAGCGATTGA