Detailed information of effector
Summary
| Effector ID | EFF00045  |
| Effector type | Tae |
| Cognate immunity protein | IMU00073 |
| Related T6SS (Type) | T6SS00035 (Type i3) |
| Strain | Pseudomonas protegens Pf-5 |
| Replicon | chromosome |
| Sequence | Protein sequence (124 a.a.); Nucleotide sequence (372 bp) |
| Description | Tae3; peptidoglycan amidase |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PFL_5498 (Tae3) |
| Coordinates (Strand) | 6278373..6278744 (+) |
| NCBI ID | 70732799 |
| RefSeq | NC_004129 |
| Uniprot ID | UPI000054E04D |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-123 | MWNKEAAISHLNAHAHAGSTGRCAAYTRQAIEAGGGGVILHRKHSAKDFGSSLTSAGFIEQPAGQTPAAGDVVIIQPIPGHPHGHMAMFNGTLWVSDFKQLHGFYPGHSYRVQKPAYKIYRHP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8556 | - | - |
Protein sequence: 124 a.a. .
>EFF00045 NC_004129:6278373-6278744 Type VI Amidase Effector (Tae) [Pseudomonas protegens Pf-5]
MWNKEAAISHLNAHAHAGSTGRCAAYTRQAIEAGGGGVILHRKHSAKDFGSSLTSAGFIEQPAGQTPAAGDVVIIQPIPG
HPHGHMAMFNGTLWVSDFKQLHGFYPGHSYRVQKPAYKIYRHP*
MWNKEAAISHLNAHAHAGSTGRCAAYTRQAIEAGGGGVILHRKHSAKDFGSSLTSAGFIEQPAGQTPAAGDVVIIQPIPG
HPHGHMAMFNGTLWVSDFKQLHGFYPGHSYRVQKPAYKIYRHP*
Nucleotide sequence: 372 bp .
>EFF00045 NC_004129:6278373-6278744 Type VI Amidase Effector (Tae) [Pseudomonas protegens Pf-5]
ATGTGGAATAAAGAAGCCGCTATCAGTCACTTGAACGCTCACGCTCACGCCGGTAGTACGGGGCGCTGTGCGGCCTATAC
TCGCCAGGCGATCGAAGCCGGTGGCGGTGGGGTGATTCTGCACAGGAAGCACTCGGCCAAGGACTTCGGCTCATCATTGA
CTTCTGCAGGTTTCATCGAACAGCCGGCCGGCCAGACGCCCGCAGCGGGCGATGTGGTGATCATCCAGCCCATTCCAGGG
CACCCCCACGGTCACATGGCGATGTTCAACGGCACACTCTGGGTGTCAGACTTCAAGCAACTGCACGGTTTCTACCCGGG
GCATTCCTACCGGGTGCAGAAACCTGCCTACAAAATCTATCGCCATCCGTAA
ATGTGGAATAAAGAAGCCGCTATCAGTCACTTGAACGCTCACGCTCACGCCGGTAGTACGGGGCGCTGTGCGGCCTATAC
TCGCCAGGCGATCGAAGCCGGTGGCGGTGGGGTGATTCTGCACAGGAAGCACTCGGCCAAGGACTTCGGCTCATCATTGA
CTTCTGCAGGTTTCATCGAACAGCCGGCCGGCCAGACGCCCGCAGCGGGCGATGTGGTGATCATCCAGCCCATTCCAGGG
CACCCCCACGGTCACATGGCGATGTTCAACGGCACACTCTGGGTGTCAGACTTCAAGCAACTGCACGGTTTCTACCCGGG
GCATTCCTACCGGGTGCAGAAACCTGCCTACAAAATCTATCGCCATCCGTAA