Detailed information of effector
Summary
| Effector ID | EFF76635  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS00004 (Type i1) |
| Strain | Pseudomonas aeruginosa PAO1 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Description | His-tag pull-down assay showed that all three proteins were co-eluted with only one 6his-tag at N-ter of TsiV. This indicates that strong interactions exist among the PAAR2 cluster encoded proteins even under the condition of 500?mm NaCl. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | PA0824 (PAAR2) |
| Coordinates (Strand) | 899138..899656 (-) |
| NCBI ID | NP_249515.1 |
| RefSeq | NC_002516 |
| Uniprot ID | Q9I5B5_PSEAE |
| KEGG ID | pae:PA0824 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| PAAR_motif | PF05488.15 | 3.4e-16 | 2..53 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MSGKPAARVTDPTTCPVPGHGSNPIVQGSPDVVFDGLPAARQGDASACGSPMISAVSSTVLINGLPAVTLGSIGAHGNVVIGGSGTVLIGDVFTPAPRAPALPLNRNSVPCSGRFQLIDHETGKPVAGRRVRVWSSGGWNAFDTTDADGMTSWIERPTAEILYIDLVQRCDA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9036 | - | - |
Protein sequence: 173 a.a. .
>EFF76635 NC_002516:c899656-899138 (Unknown) [Pseudomonas aeruginosa PAO1]
MSGKPAARVTDPTTCPVPGHGSNPIVQGSPDVVFDGLPAARQGDASACGSPMISAVSSTVLINGLPAVTLGSIGAHGNVV
IGGSGTVLIGDVFTPAPRAPALPLNRNSVPCSGRFQLIDHETGKPVAGRRVRVWSSGGWNAFDTTDADGMTSWIERPTAE
ILYIDLVQRCDA*
MSGKPAARVTDPTTCPVPGHGSNPIVQGSPDVVFDGLPAARQGDASACGSPMISAVSSTVLINGLPAVTLGSIGAHGNVV
IGGSGTVLIGDVFTPAPRAPALPLNRNSVPCSGRFQLIDHETGKPVAGRRVRVWSSGGWNAFDTTDADGMTSWIERPTAE
ILYIDLVQRCDA*
Nucleotide sequence: 519 bp .
>EFF76635 NC_002516:c899656-899138 (Unknown) [Pseudomonas aeruginosa PAO1]
ATGAGTGGAAAACCCGCCGCCCGCGTCACCGATCCAACGACCTGCCCAGTGCCAGGGCATGGCAGCAACCCCATCGTCCA
GGGCTCTCCCGACGTCGTTTTCGACGGCCTGCCGGCAGCACGGCAAGGCGACGCCTCAGCCTGCGGAAGCCCGATGATCT
CAGCCGTTTCGTCGACGGTACTCATCAACGGCCTGCCGGCGGTGACGCTGGGTAGTATCGGGGCGCACGGCAACGTGGTG
ATCGGCGGCTCGGGGACGGTACTCATCGGCGACGTCTTCACGCCCGCCCCGCGCGCTCCCGCCCTTCCCCTTAACCGCAA
CAGCGTGCCCTGTTCCGGGCGCTTCCAGCTCATCGACCACGAAACGGGCAAGCCCGTCGCCGGACGCCGGGTCAGGGTCT
GGTCGAGTGGCGGCTGGAATGCGTTCGACACCACGGATGCCGACGGCATGACCTCCTGGATCGAGCGCCCCACCGCGGAG
ATCCTCTACATCGACCTGGTGCAGAGGTGCGACGCATGA
ATGAGTGGAAAACCCGCCGCCCGCGTCACCGATCCAACGACCTGCCCAGTGCCAGGGCATGGCAGCAACCCCATCGTCCA
GGGCTCTCCCGACGTCGTTTTCGACGGCCTGCCGGCAGCACGGCAAGGCGACGCCTCAGCCTGCGGAAGCCCGATGATCT
CAGCCGTTTCGTCGACGGTACTCATCAACGGCCTGCCGGCGGTGACGCTGGGTAGTATCGGGGCGCACGGCAACGTGGTG
ATCGGCGGCTCGGGGACGGTACTCATCGGCGACGTCTTCACGCCCGCCCCGCGCGCTCCCGCCCTTCCCCTTAACCGCAA
CAGCGTGCCCTGTTCCGGGCGCTTCCAGCTCATCGACCACGAAACGGGCAAGCCCGTCGCCGGACGCCGGGTCAGGGTCT
GGTCGAGTGGCGGCTGGAATGCGTTCGACACCACGGATGCCGACGGCATGACCTCCTGGATCGAGCGCCCCACCGCGGAG
ATCCTCTACATCGACCTGGTGCAGAGGTGCGACGCATGA