Detailed information of effector


Summary

Effector ID EFF18628 experimental
Effector type Tde
Cognate immunity protein IMU17308 experimental
Related T6SS (Type) T6SS17501 experimental (Type i3)
Strain Neisseria cinerea isolate CCUG346T (346T)
Replicon chromosome
Sequence Protein sequence (1383 a.a.); Nucleotide sequence (4149 bp)
Description Nte6 predicted to contain an HNHc endonuclease active site (cd00085). All Ntes are toxic, with their expression leading to decreased viability and reduced optical density (OD) of E. coli cultures compared to empty vector controls; toxicity was counteracted by co-expression of the corresponding Nti.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) Nte6
Coordinates (Strand) 69761..73909 (+)
NCBI ID -
GenBank 346T_plasmid
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF6531 PF20148.1 2.5e-21 126..200
RHS PF03527.16 1.9e-11 1140..1172
RHS_repeat PF05593.16 3.1e-08 526..555
RHS_repeat PF05593.16 1.3e-06 625..661
RHS_repeat PF05593.16 6.3e-06 478..512
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-1382 MASFGNTFGVLIPKPAAPAMVSQGSANPPEPLTNAAGPVDVIEMVADVASTALSIVASDSAAADTVDAISELVSLASMIPGQPGPKPPSRKMVSGFSGGMGMGFDGGVVTSGHGHCIPCKVAAAIGNPVNAVLGIKVLFDDTETDFAFDSPLPLVWQRSYYSDQIGNGWLGQGWSLPFSMRLVRTADGFLYIDEQGREIPLPDISDKADEPYSAADDDEDDLYEEEAAPRPASAEEDPYGLDDAYFDPYEQIFFSQISDDLYQIASPDGGARLLFAEVDSGCGIYQLVAQLDRNGRHIRLCYDDNGLPHSIYDGSGRHFQPVFSSIRLHDNDPDFDPAGERDVFVSKDERFYVNRLTSVTFNGKELVRYDYDGYGDLTAVYGRDGKKLRGFAYRNHIMVEHNQPDGLVSRYEYDRYDTDGKVLKSSNNLGEEWTFDYRKDHTVVTDALGRTEVYGFDENHELVYRIDADGQRSDSERDSYGRITVERDPLGRETRYLYDTEGNVIAITAPDGGSTQIDYHETLNLPVAVNDPAGRITAYTYDGRGNLVSITDPAGYTTRYGYNARWLPETITDALGKTRRLHYDTLDQLVCFTDCTGETTRFAYTEYGDLETVTDALGHTTRHHYDAAGNPVRTDYPDGSHETFEYDRLNRLTAHIDGLGAQTAYELAVDGLPLKRTNALGHTFAYAYDKARRLTVLTNENGETYRLDYGPTDNLIQETGWDGKITAYGYDAAGQLVQQTEYGQSNNEGRLKDRPETWHIHRFKRNILGQLIEKQSRKVSGRNGQSKDEGISRTRFDYDPITGNLTKARNRHSSVELAYDELDHLIGETTVHNGQSATVGYQYDPLGNRIRTILPDGRHIDYLYYGSGHLHQISLDGEVVSDIERDKLHREIQRTQGSISSLYDYDPMGRLKSQRTVRSGTQTPRGKQNPPAGGAVNRRYAYDKAGNLIQSADQRSGVLHYVYDKIGRIQEARNSQSGRSETFAFDPAHNILSDKTAEGKDKGSNISSGNRLESYNGIEYTYDALGNLIYRQLPNGENQYYQYDLENQLVRAEIKKPAGNTEIWEYAYDPFGRRLSKERQDKLAWTSTDPKRTHFVWDGSRLLQEYTYKGSYTYLYTDQDSYEPLAQIFDNAKDSKQYLAYFHNDQIGIPREMTDIHGNLLWYGEYTAWGRLKKDGRVYQNVHQPFRLQNQYYDEETGLHYNLMRYYEPEAGRFVNQDPILLLGGSNLYSFASNTNAWFDPLGLKKKRNKKPRRIIPYDDIMDDVDKWSLVPVSQRQKCLIKDKLPSAKQRSVQQNRMMRKDFSRREKSLIREWERETGHTWPAGATPHHIIPLKNGGSNTWWNLTPVKHPHTGTIHGTGSALRTHLPYSTPTGIINQIVPR


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.7491 - -


  Protein sequence: 1383 a.a.    .

>EFF18628 346T_plasmid:69761-73909 Type VI DNase and RNase Effector (Tde) [synthetic DNA construct]
MASFGNTFGVLIPKPAAPAMVSQGSANPPEPLTNAAGPVDVIEMVADVASTALSIVASDSAAADTVDAISELVSLASMIP
GQPGPKPPSRKMVSGFSGGMGMGFDGGVVTSGHGHCIPCKVAAAIGNPVNAVLGIKVLFDDTETDFAFDSPLPLVWQRSY
YSDQIGNGWLGQGWSLPFSMRLVRTADGFLYIDEQGREIPLPDISDKADEPYSAADDDEDDLYEEEAAPRPASAEEDPYG
LDDAYFDPYEQIFFSQISDDLYQIASPDGGARLLFAEVDSGCGIYQLVAQLDRNGRHIRLCYDDNGLPHSIYDGSGRHFQ
PVFSSIRLHDNDPDFDPAGERDVFVSKDERFYVNRLTSVTFNGKELVRYDYDGYGDLTAVYGRDGKKLRGFAYRNHIMVE
HNQPDGLVSRYEYDRYDTDGKVLKSSNNLGEEWTFDYRKDHTVVTDALGRTEVYGFDENHELVYRIDADGQRSDSERDSY
GRITVERDPLGRETRYLYDTEGNVIAITAPDGGSTQIDYHETLNLPVAVNDPAGRITAYTYDGRGNLVSITDPAGYTTRY
GYNARWLPETITDALGKTRRLHYDTLDQLVCFTDCTGETTRFAYTEYGDLETVTDALGHTTRHHYDAAGNPVRTDYPDGS
HETFEYDRLNRLTAHIDGLGAQTAYELAVDGLPLKRTNALGHTFAYAYDKARRLTVLTNENGETYRLDYGPTDNLIQETG
WDGKITAYGYDAAGQLVQQTEYGQSNNEGRLKDRPETWHIHRFKRNILGQLIEKQSRKVSGRNGQSKDEGISRTRFDYDP
ITGNLTKARNRHSSVELAYDELDHLIGETTVHNGQSATVGYQYDPLGNRIRTILPDGRHIDYLYYGSGHLHQISLDGEVV
SDIERDKLHREIQRTQGSISSLYDYDPMGRLKSQRTVRSGTQTPRGKQNPPAGGAVNRRYAYDKAGNLIQSADQRSGVLH
YVYDKIGRIQEARNSQSGRSETFAFDPAHNILSDKTAEGKDKGSNISSGNRLESYNGIEYTYDALGNLIYRQLPNGENQY
YQYDLENQLVRAEIKKPAGNTEIWEYAYDPFGRRLSKERQDKLAWTSTDPKRTHFVWDGSRLLQEYTYKGSYTYLYTDQD
SYEPLAQIFDNAKDSKQYLAYFHNDQIGIPREMTDIHGNLLWYGEYTAWGRLKKDGRVYQNVHQPFRLQNQYYDEETGLH
YNLMRYYEPEAGRFVNQDPILLLGGSNLYSFASNTNAWFDPLGLKKKRNKKPRRIIPYDDIMDDVDKWSLVPVSQRQKCL
IKDKLPSAKQRSVQQNRMMRKDFSRREKSLIREWERETGHTWPAGATPHHIIPLKNGGSNTWWNLTPVKHPHTGTIHGTG
SALRTHLPYSTPTGIINQIVPR*

  Nucleotide sequence: 4149 bp    .

>EFF18628 346T_plasmid:69761-73909 Type VI DNase and RNase Effector (Tde) [synthetic DNA construct]
ATGGCCTCTTTCGGTAACACTTTCGGTGTTCTCATTCCTAAACCAGCCGCCCCAGCCATGGTGTCTCAAGGCTCTGCCAA
TCCACCCGAGCCGCTGACCAATGCAGCCGGTCCGGTGGATGTGATAGAAATGGTTGCCGATGTTGCTTCCACTGCCCTTT
CCATCGTTGCGTCTGATTCTGCCGCAGCTGATACAGTTGATGCCATATCCGAACTGGTTTCCCTTGCGTCCATGATACCC
GGTCAGCCGGGTCCCAAGCCCCCTTCCCGTAAAATGGTCAGCGGTTTCAGCGGTGGTATGGGTATGGGTTTTGATGGTGG
GGTGGTTACCTCGGGTCATGGTCACTGTATTCCTTGTAAGGTGGCCGCAGCTATCGGTAATCCTGTAAACGCAGTGCTCG
GTATCAAAGTCCTATTTGACGATACCGAGACCGACTTTGCCTTCGATTCCCCGCTTCCCCTTGTTTGGCAGCGCAGCTAC
TATTCCGACCAAATAGGCAACGGCTGGCTGGGGCAAGGTTGGTCGCTGCCGTTCTCCATGCGTCTTGTCCGGACTGCCGA
CGGTTTCCTCTATATTGATGAGCAGGGCAGGGAAATTCCGTTGCCGGATATCAGCGACAAAGCGGACGAGCCTTATTCCG
CAGCCGACGACGATGAAGACGATCTATATGAAGAAGAGGCTGCGCCAAGACCTGCTTCCGCCGAAGAAGATCCCTACGGT
TTGGATGACGCCTATTTTGATCCTTACGAACAAATCTTCTTTTCACAGATTTCAGACGACCTCTATCAAATCGCCTCCCC
CGACGGCGGCGCACGGCTGTTGTTTGCCGAAGTCGATTCCGGCTGCGGCATTTACCAACTGGTCGCCCAACTCGACCGCA
ACGGCCGCCATATCCGCCTCTGCTACGACGACAACGGGCTGCCGCACAGCATTTACGACGGCAGCGGCCGACATTTCCAA
CCCGTATTCTCCTCCATCCGTCTGCACGACAACGATCCCGACTTCGACCCGGCCGGAGAACGGGACGTCTTCGTTTCCAA
AGACGAACGTTTCTACGTCAACCGCCTCACCTCCGTCACCTTCAACGGCAAGGAACTGGTGCGCTACGACTACGACGGCT
ACGGCGATTTGACCGCCGTTTACGGACGCGACGGCAAAAAACTGCGCGGTTTCGCCTACCGCAACCACATCATGGTCGAA
CACAACCAGCCCGACGGACTGGTATCCCGCTACGAATACGACCGTTACGACACCGACGGCAAAGTGCTCAAAAGCAGCAA
CAACCTCGGCGAAGAATGGACGTTCGACTACCGCAAAGACCATACCGTCGTTACCGACGCATTGGGACGGACGGAAGTGT
ACGGTTTCGACGAAAACCACGAACTCGTCTACCGCATCGACGCCGACGGACAACGCAGCGACAGCGAACGCGACAGCTAC
GGCCGCATTACCGTAGAACGCGACCCCCTCGGACGCGAAACCCGCTATCTCTACGATACCGAAGGCAACGTCATCGCCAT
CACCGCCCCCGACGGCGGCAGCACCCAAATCGACTACCACGAAACCCTCAACCTGCCGGTTGCCGTCAACGATCCCGCCG
GCAGGATTACCGCCTACACCTACGACGGACGCGGCAACCTCGTCAGCATCACCGATCCCGCCGGTTACACCACCCGCTAC
GGCTACAACGCCCGATGGCTGCCCGAAACTATTACCGACGCCTTAGGCAAAACCCGACGCCTACACTACGACACCCTCGA
CCAACTCGTCTGCTTTACCGACTGTACCGGCGAAACCACCCGCTTCGCTTATACCGAATACGGCGATCTCGAAACCGTTA
CCGATGCGCTGGGCCATACCACCCGCCACCACTACGACGCAGCGGGCAACCCCGTCCGTACCGACTATCCCGACGGCAGT
CACGAAACCTTCGAATACGACCGCCTCAACCGTCTGACCGCCCACATCGACGGACTCGGTGCCCAAACCGCCTACGAACT
CGCAGTGGACGGACTGCCGCTCAAACGCACCAACGCACTGGGCCATACCTTCGCCTACGCCTACGACAAAGCCCGCCGTC
TGACCGTCCTCACCAACGAAAACGGCGAAACCTACCGTCTCGATTACGGCCCGACCGACAACCTGATCCAAGAAACCGGC
TGGGACGGCAAAATCACCGCCTACGGCTACGATGCCGCCGGACAACTGGTTCAACAGACCGAATACGGCCAAAGCAATAA
TGAAGGTCGTCTGAAAGACCGTCCCGAGACTTGGCACATCCACCGGTTCAAACGCAACATCCTCGGCCAACTGATCGAAA
AACAAAGCCGCAAAGTCTCCGGCCGCAACGGACAAAGCAAAGACGAAGGCATCAGCCGCACCCGTTTCGACTACGACCCC
ATTACCGGCAACCTGACCAAAGCCCGCAACCGACACAGCAGCGTCGAACTCGCCTACGACGAACTCGACCACCTCATCGG
CGAAACCACCGTCCACAACGGCCAAAGCGCCACCGTAGGCTATCAATACGACCCGCTGGGCAACCGCATCCGCACCATCC
TGCCCGACGGCCGCCATATCGATTACCTGTACTACGGCAGCGGACACCTGCACCAAATCAGCCTAGACGGCGAAGTCGTC
AGCGACATCGAACGCGACAAACTGCACCGCGAAATCCAAAGAACACAGGGCAGCATCAGCAGCCTGTACGACTACGACCC
CATGGGTCGTCTGAAAAGCCAGCGTACCGTCCGCAGCGGCACACAAACACCCCGCGGCAAACAAAACCCGCCGGCCGGCG
GCGCCGTCAACCGCCGCTACGCCTACGACAAAGCCGGCAACCTGATTCAAAGTGCCGACCAAAGAAGCGGCGTCCTTCAT
TACGTTTACGACAAAATCGGACGGATTCAGGAAGCCCGCAACAGCCAAAGCGGCCGCAGCGAAACCTTCGCCTTCGACCC
CGCCCACAACATCCTTTCCGACAAAACAGCCGAAGGGAAGGACAAAGGCAGTAACATTAGCAGCGGCAACCGCCTAGAAT
CATACAACGGCATCGAATACACCTACGACGCACTGGGCAACCTGATCTACCGCCAGCTGCCCAACGGTGAAAACCAATAT
TACCAATACGATTTAGAAAACCAACTCGTCCGCGCCGAAATCAAAAAGCCCGCCGGTAATACCGAGATTTGGGAATATGC
CTATGACCCGTTCGGAAGACGCCTTTCCAAAGAACGCCAAGACAAACTCGCCTGGACGAGCACCGACCCCAAACGCACCC
ACTTCGTCTGGGACGGCAGCCGCCTATTGCAGGAGTACACCTACAAAGGCAGCTACACCTATCTCTACACCGACCAAGAC
AGCTACGAACCGCTGGCGCAAATCTTTGATAACGCCAAAGACAGCAAACAATACCTCGCCTATTTCCACAACGACCAAAT
CGGCATACCGAGGGAGATGACCGACATCCACGGCAATCTCTTATGGTACGGCGAATACACCGCCTGGGGTCGTCTGAAAA
AGGACGGGCGTGTCTATCAGAACGTCCATCAGCCGTTCAGATTGCAGAACCAGTATTACGATGAAGAGACAGGGCTGCAT
TACAACCTGATGCGCTATTACGAGCCTGAGGCCGGGCGGTTTGTGAATCAGGATCCTATCTTACTATTAGGTGGGAGCAA
TTTGTATAGTTTTGCTTCCAATACAAATGCTTGGTTTGATCCATTGGGTTTGAAGAAAAAACGGAATAAGAAGCCTAGAA
GAATTATTCCATATGATGACATTATGGATGATGTTGATAAGTGGTCACTTGTTCCTGTTAGCCAAAGACAAAAATGTTTG
ATTAAGGATAAACTACCTAGTGCAAAACAAAGAAGCGTCCAACAAAATCGCATGATGCGTAAAGACTTTTCTCGAAGAGA
AAAAAGCTTAATACGAGAGTGGGAACGTGAAACAGGGCATACATGGCCTGCAGGAGCTACTCCTCATCATATAATTCCAC
TTAAAAATGGTGGTTCAAATACATGGTGGAATCTAACTCCTGTGAAACACCCTCATACTGGAACTATACACGGTACTGGG
TCTGCCCTACGAACACATTTGCCATATAGCACACCAACAGGAATAATTAATCAGATTGTTCCTAGATAA