Detailed information of effector


Summary

Effector ID EFF01902 experimental
Effector type Tpe
Cognate immunity protein -
Related T6SS (Type) T6SS01300 experimental (Type i4b)
Strain Burkholderia gladioli NGJ1
Replicon chromosome
Sequence Protein sequence (878 a.a.); Nucleotide sequence (2634 bp)
Description The ectopic expression of selected effector (9tle, 13tle, 23tge, and 12tpe) inhibited the growth of recombinant E. coli (BL21) cells while co-expression with their cognate immunity protein (9tli, 13tli, 23tgi or 12tpi) using different plasmids protected the cells from effector mediated killing.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACI79_RS09760 (12tpe)
Coordinates (Strand) 79857..82490 (+)
NCBI ID 836689122
GenBank LEKY01000008
Uniprot ID UPI00064B1D83
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-794 MATIPGCPICDRDSLLIYPVRYAIACPRGAAKAPALSGNFRIDARAPQAVATAKYTLRSLRQGYLYTYDEKRKKLRAYMVLDDGVLYQFAPSVVPPPGNAANNLTQNICPSGDMAPESYGRCVGVQHTPGNDEATNFWIGWSNVRWTRDTVFNKIGDATWRKQHMQCINVSAMVAGSAADTGEFQATRTQIAHFAMDDRAMKEAFGFSNRSPKDEIKLRQRHADARIGDIMAASPLKKGFVVAVNDPVGMTNDLSELTVPNLNNGFDAELYWKYVSAQLLERAEAGIRANAKAITGVTYGTSKVIADANAVNAAARAPVEPAADPIGFYHFVRSWIKTGSADQAAKIEQQKIDDIPATQQDAANEAWGEAYYKKGPDGKPQIGPDGKPVTVIDTQALQRFRDNEYPHALDAFKPKWAPLVQAHADWLKSQLLADWMAGNHDTKDLRSGYAYSESCAQSIGAAAGTEACQKVLNDWLNGQASDARNLYVRALMFNQETLMKAADAQVHGSDIQYENILNLYKEAFKQIEKLGNAANLRDRLVVTTANTIVNVLAQGAKGAAIGFATIRLSLHAGVSIKAAHITSAEMRNWIFKQGDALGLNMDGDQLERQAAAANIRREVFKAAPPSNPAVFAYAIDTDAWVKSGKLDASDVKAIKLPGVEITRKWLGSSSPVEFNQGIATAIFQLATLRFAYKDFDGSDSFNYNETLLKLSGAVVSIIGNVIETVSETVAKAPEHPLSAFLMKHWAWFNHDTAEIAAKVGRWSGAVAGVVLSCFDLFKNTPEAYGNKEYGLTAL
Transmembrane helix 795-817 YVVSGTLGVYVALSPLLSAVPFI
Inside 818-823 AAWMPP
Transmembrane helix 824-846 LWPILVISILIGFVIVYFKSAAI
Outside 847-877 NDWVSRCKFSKEPRYRTMDEELKAFNSAMGA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9293 - -


  Protein sequence: 878 a.a.    .

>EFF01902 LEKY01000008:79857-82490 Type VI Peptidase Effector (Tpe) [Burkholderia gladioli]
MATIPGCPICDRDSLLIYPVRYAIACPRGAAKAPALSGNFRIDARAPQAVATAKYTLRSLRQGYLYTYDEKRKKLRAYMV
LDDGVLYQFAPSVVPPPGNAANNLTQNICPSGDMAPESYGRCVGVQHTPGNDEATNFWIGWSNVRWTRDTVFNKIGDATW
RKQHMQCINVSAMVAGSAADTGEFQATRTQIAHFAMDDRAMKEAFGFSNRSPKDEIKLRQRHADARIGDIMAASPLKKGF
VVAVNDPVGMTNDLSELTVPNLNNGFDAELYWKYVSAQLLERAEAGIRANAKAITGVTYGTSKVIADANAVNAAARAPVE
PAADPIGFYHFVRSWIKTGSADQAAKIEQQKIDDIPATQQDAANEAWGEAYYKKGPDGKPQIGPDGKPVTVIDTQALQRF
RDNEYPHALDAFKPKWAPLVQAHADWLKSQLLADWMAGNHDTKDLRSGYAYSESCAQSIGAAAGTEACQKVLNDWLNGQA
SDARNLYVRALMFNQETLMKAADAQVHGSDIQYENILNLYKEAFKQIEKLGNAANLRDRLVVTTANTIVNVLAQGAKGAA
IGFATIRLSLHAGVSIKAAHITSAEMRNWIFKQGDALGLNMDGDQLERQAAAANIRREVFKAAPPSNPAVFAYAIDTDAW
VKSGKLDASDVKAIKLPGVEITRKWLGSSSPVEFNQGIATAIFQLATLRFAYKDFDGSDSFNYNETLLKLSGAVVSIIGN
VIETVSETVAKAPEHPLSAFLMKHWAWFNHDTAEIAAKVGRWSGAVAGVVLSCFDLFKNTPEAYGNKEYGLTALYVVSGT
LGVYVALSPLLSAVPFIAAWMPPLWPILVISILIGFVIVYFKSAAINDWVSRCKFSKEPRYRTMDEELKAFNSAMGA*

  Nucleotide sequence: 2634 bp    .

>EFF01902 LEKY01000008:79857-82490 Type VI Peptidase Effector (Tpe) [Burkholderia gladioli]
ATGGCAACCATCCCGGGCTGTCCGATCTGCGATCGCGACTCACTGCTGATCTACCCCGTCCGCTACGCAATTGCGTGTCC
ACGCGGTGCCGCCAAAGCGCCCGCGCTGTCCGGTAATTTCCGGATCGACGCGCGCGCCCCGCAGGCCGTCGCGACAGCCA
AATACACCTTGCGCTCGCTGCGGCAAGGCTACTTGTACACGTACGACGAAAAGCGCAAGAAACTGCGTGCCTACATGGTC
CTGGATGACGGTGTGCTGTATCAGTTCGCCCCCAGCGTCGTGCCGCCGCCCGGTAATGCGGCGAACAACCTGACCCAGAA
TATTTGCCCGTCCGGCGACATGGCCCCTGAGTCCTACGGCCGTTGCGTAGGCGTTCAACATACCCCGGGCAACGACGAAG
CGACGAACTTCTGGATCGGCTGGTCAAACGTACGCTGGACCCGCGATACGGTCTTCAACAAAATCGGCGACGCGACGTGG
CGCAAGCAGCATATGCAGTGCATCAACGTTTCGGCGATGGTCGCCGGCAGCGCGGCCGACACGGGCGAGTTTCAGGCGAC
GCGCACCCAGATCGCGCACTTCGCGATGGACGATCGTGCGATGAAAGAGGCGTTCGGCTTCAGCAATCGCTCGCCGAAGG
ACGAAATCAAGTTACGCCAGCGTCACGCCGACGCACGCATCGGCGACATCATGGCGGCGTCCCCCCTCAAGAAAGGCTTT
GTCGTCGCCGTCAACGATCCGGTTGGAATGACAAACGATCTATCCGAACTCACGGTCCCGAATCTGAACAACGGGTTTGA
CGCGGAGCTCTACTGGAAATACGTCTCCGCACAACTTCTGGAGCGTGCCGAAGCGGGTATTCGAGCGAATGCGAAAGCGA
TAACCGGAGTGACTTACGGCACATCGAAAGTTATCGCCGATGCCAATGCCGTCAACGCGGCGGCCCGCGCGCCGGTCGAG
CCGGCCGCCGACCCGATTGGTTTTTATCACTTTGTTCGCTCCTGGATCAAGACTGGCAGCGCCGATCAGGCAGCGAAGAT
CGAGCAACAGAAAATCGATGATATTCCGGCCACGCAGCAAGACGCGGCTAATGAAGCATGGGGCGAGGCGTATTACAAGA
AAGGGCCGGACGGCAAGCCGCAGATCGGCCCGGACGGTAAACCCGTGACCGTGATCGATACCCAAGCGCTGCAACGCTTC
CGCGATAACGAGTACCCGCATGCGCTCGACGCGTTCAAGCCGAAATGGGCACCACTCGTGCAGGCGCACGCCGACTGGCT
CAAATCACAGTTGCTTGCGGACTGGATGGCCGGCAATCACGATACGAAGGATCTGCGCAGCGGCTACGCATACAGCGAAT
CGTGTGCGCAGTCGATCGGGGCTGCGGCCGGCACGGAAGCGTGCCAGAAAGTGCTCAATGACTGGCTGAACGGTCAGGCA
TCGGATGCCCGTAATCTGTATGTGCGTGCGCTGATGTTCAATCAGGAAACCCTGATGAAGGCGGCCGATGCGCAGGTACA
CGGGTCCGACATTCAGTACGAGAACATCCTGAACCTGTACAAGGAAGCGTTCAAGCAGATCGAGAAACTGGGTAACGCCG
CGAACCTGCGTGACCGGCTCGTCGTAACGACCGCCAATACGATCGTCAATGTGCTGGCGCAGGGCGCAAAAGGTGCGGCC
ATCGGATTCGCGACGATCCGATTGTCGCTGCATGCCGGCGTGTCAATCAAGGCAGCCCACATTACATCGGCCGAGATGCG
TAACTGGATATTCAAGCAGGGCGACGCGCTGGGCTTGAATATGGATGGCGACCAGCTCGAACGGCAGGCCGCTGCGGCCA
ATATCCGGCGCGAGGTTTTCAAGGCTGCACCACCTTCCAATCCGGCCGTGTTCGCCTATGCGATCGATACCGATGCATGG
GTGAAGTCGGGCAAGCTGGATGCCAGTGACGTTAAAGCGATCAAGCTTCCGGGCGTGGAGATCACCCGCAAATGGCTTGG
GTCGTCGTCGCCTGTCGAATTCAATCAGGGGATTGCCACCGCAATCTTCCAGCTCGCAACGCTGCGATTCGCGTACAAGG
ATTTCGACGGCAGCGATAGCTTTAACTATAACGAAACCCTGCTCAAGCTTTCGGGGGCGGTCGTCTCGATCATCGGTAAC
GTCATTGAGACTGTTTCCGAAACCGTCGCCAAGGCCCCCGAGCACCCGCTCTCGGCTTTCCTCATGAAACATTGGGCGTG
GTTCAATCACGATACGGCTGAAATCGCCGCCAAGGTCGGTCGATGGTCCGGCGCAGTTGCGGGGGTTGTCCTCTCATGCT
TTGACCTGTTCAAAAATACTCCGGAAGCATACGGGAACAAGGAGTACGGACTGACTGCTCTCTATGTGGTCAGCGGGACT
CTCGGAGTGTATGTTGCGCTCTCCCCACTTCTCTCGGCGGTACCGTTCATCGCCGCTTGGATGCCTCCTCTTTGGCCGAT
TCTAGTAATTTCTATTTTGATCGGCTTCGTGATCGTGTACTTCAAATCGGCGGCTATTAATGATTGGGTATCACGGTGCA
AATTCAGCAAGGAGCCGAGGTATCGCACTATGGACGAAGAGTTAAAAGCCTTCAATTCCGCAATGGGGGCTTGA