Detailed information of effector
Summary
| Effector ID | EFF01901  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS01300 (Type i4b) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Description | Western blot analysis revealed that wild-type NGJ1 bacterium is capable of secreting Hcp-1 (that is associated with T6SS-1 cluster) and Hcp-2 (that is associated with T6SS-2 cluster) proteins into extracellular milieu. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | AC179_RS13930 (Hcp-1) |
| Coordinates (Strand) | 95172..95690 (+) |
| NCBI ID | 503461802 |
| GenBank | LEKY01000012 |
| Uniprot ID | A0A095XG85_BURGA, F2LI26_BURGS |
| KEGG ID | bgo:BM43_1797, bgd:bgla_1g04100 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_VipA | PF05591.14 | 3.1e-60 | 12..163 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPRAAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQALAKQSGGGDKEGE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9963 | - | - |
Protein sequence: 173 a.a. .
>EFF01901 LEKY01000012:95172-95690 (Unknown) [Burkholderia gladioli]
MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPR
AAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQAL
AKQSGGGDKEGE*
MAKKESIQKRLQKVRPPRVQLTYEVERGDAIEVKELPFVVGVVGDLAGQSEVEMPKLRDRKFVNIDRDNFDDVMKAIEPR
AAYQVENKLSEEGGKFAVDLKFRSLDDFDPDQVVNQIEPLRRLIEARSKLADLRNKLAGNDKLEDLLSEVLSNTQQLQAL
AKQSGGGDKEGE*
Nucleotide sequence: 519 bp .
>EFF01901 LEKY01000012:95172-95690 (Unknown) [Burkholderia gladioli]
ATGGCGAAAAAAGAAAGTATCCAGAAGCGCCTGCAAAAGGTACGGCCGCCCCGTGTGCAGCTGACCTACGAAGTCGAGCG
CGGCGACGCGATCGAGGTGAAGGAACTGCCGTTCGTGGTCGGCGTGGTCGGCGACCTGGCCGGTCAGTCCGAGGTCGAGA
TGCCGAAGCTGCGCGACCGCAAGTTCGTCAACATCGATCGCGACAACTTCGACGACGTGATGAAGGCGATCGAGCCTCGC
GCGGCCTACCAGGTCGAGAACAAACTCAGCGAGGAAGGCGGCAAGTTCGCCGTCGACCTCAAGTTCCGTTCGCTCGACGA
TTTCGATCCGGACCAGGTGGTCAACCAGATCGAACCGCTGCGCCGCCTGATCGAGGCGCGCTCGAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTCGAGGACCTGCTGAGCGAAGTGCTCAGCAACACGCAGCAGTTGCAGGCGCTC
GCGAAGCAGTCGGGCGGCGGCGACAAAGAGGGCGAATAA
ATGGCGAAAAAAGAAAGTATCCAGAAGCGCCTGCAAAAGGTACGGCCGCCCCGTGTGCAGCTGACCTACGAAGTCGAGCG
CGGCGACGCGATCGAGGTGAAGGAACTGCCGTTCGTGGTCGGCGTGGTCGGCGACCTGGCCGGTCAGTCCGAGGTCGAGA
TGCCGAAGCTGCGCGACCGCAAGTTCGTCAACATCGATCGCGACAACTTCGACGACGTGATGAAGGCGATCGAGCCTCGC
GCGGCCTACCAGGTCGAGAACAAACTCAGCGAGGAAGGCGGCAAGTTCGCCGTCGACCTCAAGTTCCGTTCGCTCGACGA
TTTCGATCCGGACCAGGTGGTCAACCAGATCGAACCGCTGCGCCGCCTGATCGAGGCGCGCTCGAAGCTGGCCGACCTGC
GCAACAAGCTGGCCGGCAACGACAAGCTCGAGGACCTGCTGAGCGAAGTGCTCAGCAACACGCAGCAGTTGCAGGCGCTC
GCGAAGCAGTCGGGCGGCGGCGACAAAGAGGGCGAATAA