Detailed information of effector


Summary

Effector ID EFF01899 experimental
Effector type Tle
Cognate immunity protein -
Related T6SS (Type) T6SS01300 experimental (Type i4b)
Strain Burkholderia gladioli NGJ1
Replicon chromosome
Sequence Protein sequence (531 a.a.); Nucleotide sequence (1593 bp)
Description The ectopic expression of selected effector (9tle, 13tle, 23tge, and 12tpe) inhibited the growth of recombinant E. coli (BL21) cells while co-expression with their cognate immunity protein (9tli, 13tli, 23tgi or 12tpi) using different plasmids protected the cells from effector mediated killing.
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) ACI79_RS23485 (9tle)
Coordinates (Strand) 142254..143846 (+)
NCBI ID 836691007
GenBank LEKY01000068
Uniprot ID UPI00064A432E
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DUF2235 PF09994.11 2e-20 200..302
DUF2235 PF09994.11 1.6e-10 48..182
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-530 MSIRQAGAPATDDEIKRAAIDGSTKRILRQQPIDRADSIACRLYPALSFFFDGTNNNMERDRPLNKHSNVAKLFRAALIPNRFELKPTYLPGVGTSFKFIKVAGYTDHLKDDEGGVLGLGLGAGGDLRIRFALGEFSRLLEVEFGPGSWAHMREVTVAIFGFSRGATEARAFARRLIEQKCINRNGLFYWRAPNGVHIPLRINFMGIFDTVASVGGPALHLDWASELAIPPAVERCMHFVAAHEVRRAFPLDSVRIDAAYPSNCAEVVYPGVHSDVGGGYGPDEQGRDKDLSYVPLRHMLAEALRAGVPMMALDEMPDDSISDFQLDDDAPVVNLYNDYMAALPAAPGSDLESLIQPHRYLNFRWRSILARHQADERVLGHLYEKVGAAFCSTVPAGSDEDHPACQPNEWVYDVPKNPEEQAKQLLREQRRLERHIEFLRNPIEPRAGNRSYPPQPRELRPYEKMILAAWDEAGTIPLAVDQLLAEHIHESVAAFTSWPCALWDQRGIYCDRTRYMAQNDPITPDDAAVA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9770 - -


  Protein sequence: 531 a.a.    .

>EFF01899 LEKY01000068:142254-143846 Type VI Lipase Effector (Tle) [Burkholderia gladioli]
MSIRQAGAPATDDEIKRAAIDGSTKRILRQQPIDRADSIACRLYPALSFFFDGTNNNMERDRPLNKHSNVAKLFRAALIP
NRFELKPTYLPGVGTSFKFIKVAGYTDHLKDDEGGVLGLGLGAGGDLRIRFALGEFSRLLEVEFGPGSWAHMREVTVAIF
GFSRGATEARAFARRLIEQKCINRNGLFYWRAPNGVHIPLRINFMGIFDTVASVGGPALHLDWASELAIPPAVERCMHFV
AAHEVRRAFPLDSVRIDAAYPSNCAEVVYPGVHSDVGGGYGPDEQGRDKDLSYVPLRHMLAEALRAGVPMMALDEMPDDS
ISDFQLDDDAPVVNLYNDYMAALPAAPGSDLESLIQPHRYLNFRWRSILARHQADERVLGHLYEKVGAAFCSTVPAGSDE
DHPACQPNEWVYDVPKNPEEQAKQLLREQRRLERHIEFLRNPIEPRAGNRSYPPQPRELRPYEKMILAAWDEAGTIPLAV
DQLLAEHIHESVAAFTSWPCALWDQRGIYCDRTRYMAQNDPITPDDAAVA*

  Nucleotide sequence: 1593 bp    .

>EFF01899 LEKY01000068:142254-143846 Type VI Lipase Effector (Tle) [Burkholderia gladioli]
ATGTCTATTAGACAAGCTGGGGCGCCGGCCACCGACGACGAAATCAAACGGGCTGCAATTGATGGCTCCACAAAGCGCAT
CTTGCGTCAACAGCCTATTGATCGGGCAGATTCCATTGCTTGTCGACTTTACCCGGCCTTGTCTTTCTTTTTCGATGGTA
CGAATAACAATATGGAGCGTGATAGGCCGCTCAATAAACATTCAAATGTTGCAAAATTGTTTCGCGCGGCACTGATCCCG
AATCGATTTGAATTAAAGCCCACTTATCTTCCCGGCGTAGGAACATCATTCAAATTCATCAAAGTGGCTGGCTATACGGA
TCATTTGAAAGATGATGAGGGCGGGGTGCTTGGGCTTGGGCTTGGAGCCGGTGGAGATCTACGAATCAGGTTTGCCCTTG
GCGAGTTCTCGCGATTGCTGGAGGTCGAATTTGGGCCGGGATCATGGGCACATATGCGTGAAGTCACTGTGGCGATTTTC
GGGTTCTCGCGTGGTGCGACTGAGGCCCGGGCCTTTGCGCGGAGACTCATTGAGCAGAAATGTATCAATAGAAATGGTCT
GTTCTATTGGCGGGCGCCAAATGGTGTTCATATACCGCTGCGAATCAATTTCATGGGGATTTTTGACACGGTTGCTTCCG
TTGGAGGGCCAGCCTTACATTTGGATTGGGCATCAGAACTAGCAATTCCACCAGCGGTGGAGCGTTGCATGCACTTTGTT
GCGGCACATGAGGTACGACGAGCATTTCCACTCGATTCAGTGCGAATCGATGCGGCTTATCCAAGTAACTGCGCCGAGGT
AGTGTATCCGGGCGTACATTCTGACGTCGGCGGTGGCTACGGTCCCGACGAGCAAGGACGCGACAAGGATCTGTCTTATG
TCCCATTACGTCACATGCTTGCCGAGGCGCTGCGAGCCGGGGTGCCAATGATGGCTCTCGATGAGATGCCAGATGACAGT
ATTTCAGACTTCCAATTGGACGACGACGCGCCCGTCGTGAATCTCTACAACGACTACATGGCCGCGCTCCCCGCAGCACC
CGGCAGCGACCTCGAATCCTTGATCCAACCTCACCGTTATCTGAATTTCCGGTGGCGCAGCATCCTGGCTCGCCACCAGG
CGGACGAGCGTGTGCTGGGGCATCTATACGAAAAGGTCGGTGCCGCCTTTTGCTCCACCGTCCCGGCCGGATCCGACGAA
GACCACCCAGCCTGCCAGCCGAACGAATGGGTATATGACGTCCCGAAGAACCCGGAAGAACAGGCCAAGCAGCTTTTACG
CGAGCAGCGACGACTGGAGCGGCATATCGAATTCCTTCGCAATCCGATCGAGCCGCGAGCCGGCAATCGCTCATATCCTC
CGCAGCCGCGCGAGTTGCGCCCCTACGAAAAAATGATCCTGGCGGCCTGGGACGAGGCCGGCACGATACCCCTGGCGGTA
GATCAGCTATTGGCGGAACACATTCACGAATCGGTTGCCGCCTTCACGTCCTGGCCGTGTGCCCTTTGGGATCAACGTGG
GATTTACTGCGACAGGACACGCTACATGGCGCAAAACGATCCCATCACACCCGACGACGCGGCTGTCGCTTGA