Detailed information of effector
Summary
| Effector ID | EFF01897  |
| Effector type | Tge |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS01300 (Type i4b) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (393 a.a.); Nucleotide sequence (1179 bp) |
| Description | The ectopic expression of selected effector (9tle, 13tle, 23tge, and 12tpe) inhibited the growth of recombinant E. coli (BL21) cells while co-expression with their cognate immunity protein (9tli, 13tli, 23tgi or 12tpi) using different plasmids protected the cells from effector mediated killing. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | ACI79_RS29395 (23tge) |
| Coordinates (Strand) | 105319..106497 (+) |
| NCBI ID | 920807605 |
| GenBank | LEKY01000073 |
| Uniprot ID | UPI00069F8CD0 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-392 | VGLACRAWFLVTIGAKDGSTRQGWVCEQDHPLVRMCGPWDWPGFELIDNSSIMPLDMLKRYIHVTEQYLADESKTEFEASAATVNAGPLITNLEKAIDTDHDGKVTALELKHAQEMPWMAEALSHLVVRCESEWGGGLGKWEALSPLMKKLLWLWKTEIERIGKLQWWEQVASVEGFPKEPSPWHFHPIGIAGNFIKSGGGPLDKLIKKLGDVISHGEGGYDAYNTGTHGQSGPVVHAYTDPPAGTITRQKIKDILASGSKSPDATDRYFTSGKYQTTVYTLAEAVKKMGLTGEEIYDADMQERVFREFLIYHAGGGKLAAFIFKNVGSLTDAQYAASQEWASIAAPEGTKIKDGRISDGTLSYYEKAKQNHANPRSTAMVIAVLEEIDRSR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7754 | - | - |
Protein sequence: 393 a.a. .
>EFF01897 LEKY01000073:105319-106497 Type VI Glycoside hydrolase Effector (Tge) [Burkholderia gladioli]
VGLACRAWFLVTIGAKDGSTRQGWVCEQDHPLVRMCGPWDWPGFELIDNSSIMPLDMLKRYIHVTEQYLADESKTEFEAS
AATVNAGPLITNLEKAIDTDHDGKVTALELKHAQEMPWMAEALSHLVVRCESEWGGGLGKWEALSPLMKKLLWLWKTEIE
RIGKLQWWEQVASVEGFPKEPSPWHFHPIGIAGNFIKSGGGPLDKLIKKLGDVISHGEGGYDAYNTGTHGQSGPVVHAYT
DPPAGTITRQKIKDILASGSKSPDATDRYFTSGKYQTTVYTLAEAVKKMGLTGEEIYDADMQERVFREFLIYHAGGGKLA
AFIFKNVGSLTDAQYAASQEWASIAAPEGTKIKDGRISDGTLSYYEKAKQNHANPRSTAMVIAVLEEIDRSR*
VGLACRAWFLVTIGAKDGSTRQGWVCEQDHPLVRMCGPWDWPGFELIDNSSIMPLDMLKRYIHVTEQYLADESKTEFEAS
AATVNAGPLITNLEKAIDTDHDGKVTALELKHAQEMPWMAEALSHLVVRCESEWGGGLGKWEALSPLMKKLLWLWKTEIE
RIGKLQWWEQVASVEGFPKEPSPWHFHPIGIAGNFIKSGGGPLDKLIKKLGDVISHGEGGYDAYNTGTHGQSGPVVHAYT
DPPAGTITRQKIKDILASGSKSPDATDRYFTSGKYQTTVYTLAEAVKKMGLTGEEIYDADMQERVFREFLIYHAGGGKLA
AFIFKNVGSLTDAQYAASQEWASIAAPEGTKIKDGRISDGTLSYYEKAKQNHANPRSTAMVIAVLEEIDRSR*
Nucleotide sequence: 1179 bp .
>EFF01897 LEKY01000073:105319-106497 Type VI Glycoside hydrolase Effector (Tge) [Burkholderia gladioli]
GTGGGACTGGCCTGTCGCGCGTGGTTCCTCGTGACGATCGGCGCGAAGGACGGCAGCACACGGCAGGGATGGGTCTGTGA
GCAAGATCATCCGCTGGTGCGGATGTGCGGGCCGTGGGACTGGCCTGGCTTCGAATTGATCGACAACAGCAGCATCATGC
CGCTCGATATGCTGAAGCGTTACATCCATGTCACCGAGCAGTACCTGGCTGACGAAAGCAAGACCGAGTTCGAGGCGAGC
GCGGCGACGGTCAATGCCGGCCCGCTGATCACGAACCTCGAGAAGGCGATTGATACAGACCACGATGGGAAGGTCACCGC
GCTCGAGCTCAAGCATGCTCAGGAAATGCCGTGGATGGCTGAGGCGCTATCGCATCTGGTGGTACGGTGCGAAAGCGAAT
GGGGCGGCGGCCTGGGCAAATGGGAGGCGCTGTCGCCGCTGATGAAGAAGTTGCTGTGGCTGTGGAAGACGGAGATCGAG
CGGATTGGGAAGCTGCAGTGGTGGGAGCAGGTGGCCAGCGTGGAGGGGTTTCCGAAGGAACCGAGCCCCTGGCACTTTCA
TCCGATAGGGATTGCCGGCAATTTCATTAAATCAGGCGGCGGCCCACTGGATAAATTGATAAAGAAACTCGGAGACGTAA
TTTCGCATGGGGAAGGTGGGTATGACGCCTACAATACCGGTACTCACGGTCAAAGCGGCCCTGTTGTTCACGCTTACACC
GATCCTCCAGCGGGCACAATCACGCGGCAAAAAATTAAGGACATTCTCGCAAGCGGCTCCAAATCCCCGGATGCAACGGA
CCGGTATTTTACGTCTGGAAAATACCAGACAACTGTGTACACGCTAGCCGAAGCAGTCAAAAAGATGGGCTTGACAGGGG
AAGAAATTTACGATGCAGATATGCAAGAGCGGGTGTTTAGAGAGTTCTTAATCTATCATGCTGGGGGAGGGAAGCTTGCG
GCCTTTATATTCAAGAATGTAGGTTCGCTAACCGACGCGCAATATGCGGCATCTCAGGAATGGGCGTCCATAGCTGCACC
GGAAGGCACCAAAATCAAAGATGGCAGGATCTCGGATGGCACATTAAGTTACTATGAGAAAGCAAAGCAGAACCATGCGA
ACCCCAGGTCGACCGCAATGGTGATAGCAGTGCTTGAGGAAATCGACAGGAGCCGTTAG
GTGGGACTGGCCTGTCGCGCGTGGTTCCTCGTGACGATCGGCGCGAAGGACGGCAGCACACGGCAGGGATGGGTCTGTGA
GCAAGATCATCCGCTGGTGCGGATGTGCGGGCCGTGGGACTGGCCTGGCTTCGAATTGATCGACAACAGCAGCATCATGC
CGCTCGATATGCTGAAGCGTTACATCCATGTCACCGAGCAGTACCTGGCTGACGAAAGCAAGACCGAGTTCGAGGCGAGC
GCGGCGACGGTCAATGCCGGCCCGCTGATCACGAACCTCGAGAAGGCGATTGATACAGACCACGATGGGAAGGTCACCGC
GCTCGAGCTCAAGCATGCTCAGGAAATGCCGTGGATGGCTGAGGCGCTATCGCATCTGGTGGTACGGTGCGAAAGCGAAT
GGGGCGGCGGCCTGGGCAAATGGGAGGCGCTGTCGCCGCTGATGAAGAAGTTGCTGTGGCTGTGGAAGACGGAGATCGAG
CGGATTGGGAAGCTGCAGTGGTGGGAGCAGGTGGCCAGCGTGGAGGGGTTTCCGAAGGAACCGAGCCCCTGGCACTTTCA
TCCGATAGGGATTGCCGGCAATTTCATTAAATCAGGCGGCGGCCCACTGGATAAATTGATAAAGAAACTCGGAGACGTAA
TTTCGCATGGGGAAGGTGGGTATGACGCCTACAATACCGGTACTCACGGTCAAAGCGGCCCTGTTGTTCACGCTTACACC
GATCCTCCAGCGGGCACAATCACGCGGCAAAAAATTAAGGACATTCTCGCAAGCGGCTCCAAATCCCCGGATGCAACGGA
CCGGTATTTTACGTCTGGAAAATACCAGACAACTGTGTACACGCTAGCCGAAGCAGTCAAAAAGATGGGCTTGACAGGGG
AAGAAATTTACGATGCAGATATGCAAGAGCGGGTGTTTAGAGAGTTCTTAATCTATCATGCTGGGGGAGGGAAGCTTGCG
GCCTTTATATTCAAGAATGTAGGTTCGCTAACCGACGCGCAATATGCGGCATCTCAGGAATGGGCGTCCATAGCTGCACC
GGAAGGCACCAAAATCAAAGATGGCAGGATCTCGGATGGCACATTAAGTTACTATGAGAAAGCAAAGCAGAACCATGCGA
ACCCCAGGTCGACCGCAATGGTGATAGCAGTGCTTGAGGAAATCGACAGGAGCCGTTAG