Detailed information of effector
Summary
| Effector ID | EFF01896  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS01301 (Type i3) |
| Strain | Burkholderia gladioli NGJ1 |
| Replicon | chromosome |
| Sequence | Protein sequence (168 a.a.); Nucleotide sequence (504 bp) |
| Description | T6SS-2 strain was defective in secretion of Hcp-2 but proficient in secretion of Hcp-1. |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | AC179_RS29610 (Hcp-2) |
| Coordinates (Strand) | 57344..57847 (+) |
| NCBI ID | 738096046 |
| GenBank | LEKY01000074 |
| Uniprot ID | A0A095FFB5_BURGA |
| KEGG ID | bgo:BM43_7145 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 2.4e-27 | 12..140 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-167 | MAFDMHLKFGSGSVTIQGASNHSKHKNEVPIIAWSWGVSNTGNLHTGAGYASGGKANIQDITVTKYIDSCSNAILNACCTGARVDNAYLYVTNATGQQTDFVTIELSEGVLITSVSTGGTGGDERLTENITLHFGKFKYSFQPQDDEGKANGGTKDFTYDIQQVAKQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8585 | - | - |
Protein sequence: 168 a.a. .
>EFF01896 LEKY01000074:57344-57847 (Unknown) [Burkholderia gladioli]
MAFDMHLKFGSGSVTIQGASNHSKHKNEVPIIAWSWGVSNTGNLHTGAGYASGGKANIQDITVTKYIDSCSNAILNACCT
GARVDNAYLYVTNATGQQTDFVTIELSEGVLITSVSTGGTGGDERLTENITLHFGKFKYSFQPQDDEGKANGGTKDFTYD
IQQVAKQ*
MAFDMHLKFGSGSVTIQGASNHSKHKNEVPIIAWSWGVSNTGNLHTGAGYASGGKANIQDITVTKYIDSCSNAILNACCT
GARVDNAYLYVTNATGQQTDFVTIELSEGVLITSVSTGGTGGDERLTENITLHFGKFKYSFQPQDDEGKANGGTKDFTYD
IQQVAKQ*
Nucleotide sequence: 504 bp .
>EFF01896 LEKY01000074:57344-57847 (Unknown) [Burkholderia gladioli]
ATGGCATTCGACATGCACCTGAAATTCGGCTCCGGTTCGGTCACGATCCAGGGCGCGTCCAACCATTCGAAGCACAAGAA
CGAGGTGCCGATCATCGCGTGGTCGTGGGGCGTGAGCAACACCGGCAACCTGCACACCGGCGCCGGCTACGCCTCGGGCG
GCAAGGCCAACATCCAGGACATCACGGTCACCAAGTACATCGACAGCTGCTCGAACGCGATCCTGAACGCCTGCTGTACC
GGCGCGCGCGTCGACAACGCCTACCTGTACGTGACCAACGCCACCGGCCAGCAGACCGACTTCGTGACCATCGAGCTCAG
CGAAGGCGTGCTGATCACCTCGGTGTCGACCGGCGGCACCGGCGGCGACGAGCGCCTGACCGAGAACATCACGCTGCACT
TCGGCAAGTTCAAGTACTCGTTCCAGCCGCAGGACGACGAGGGCAAGGCCAACGGCGGCACCAAGGACTTCACCTACGAC
ATCCAGCAGGTCGCCAAGCAGTAA
ATGGCATTCGACATGCACCTGAAATTCGGCTCCGGTTCGGTCACGATCCAGGGCGCGTCCAACCATTCGAAGCACAAGAA
CGAGGTGCCGATCATCGCGTGGTCGTGGGGCGTGAGCAACACCGGCAACCTGCACACCGGCGCCGGCTACGCCTCGGGCG
GCAAGGCCAACATCCAGGACATCACGGTCACCAAGTACATCGACAGCTGCTCGAACGCGATCCTGAACGCCTGCTGTACC
GGCGCGCGCGTCGACAACGCCTACCTGTACGTGACCAACGCCACCGGCCAGCAGACCGACTTCGTGACCATCGAGCTCAG
CGAAGGCGTGCTGATCACCTCGGTGTCGACCGGCGGCACCGGCGGCGACGAGCGCCTGACCGAGAACATCACGCTGCACT
TCGGCAAGTTCAAGTACTCGTTCCAGCCGCAGGACGACGAGGGCAAGGCCAACGGCGGCACCAAGGACTTCACCTACGAC
ATCCAGCAGGTCGCCAAGCAGTAA