SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01860
Effector type	Tae
Cognate immunity protein	-
Related T6SS (Type)	T6SS01243 (Type i5)
Strain	Agrobacterium fabrum strain 12D13
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Description	Hcp and Tae were detected in the media fraction of each strain while Hcp was not detected in the media fractions of any of the ΔtssL mutants but could be detected in the media fractions of mutant strains complemented with their cognate tssL gene
Reference	[1] Wu CF, Santos MNM, Cho ST, et al. Plant-Pathogenic Agrobacterium tumefaciens Strains Have Diverse Type VI Effector-Immunity Pairs and Vary in In-Planta Competitiveness. Mol Plant Microbe Interact. 2019 Aug;32(8):961-971. doi: 10.1094/MPMI-01-19-0021-R. Epub 2019 Jun 27. PMID: 30830835 [2] Wu CF, Santos MNM, Cho ST, et al. Plant-Pathogenic Agrobacterium tumefaciens Strains Have Diverse Type VI Effector-Immunity Pairs and Vary in In-Planta Competitiveness. Mol Plant Microbe Interact. 2019 Aug;32(8):961-971. doi: 10.1094/MPMI-01-19-0021-R. Epub 2019 Jun 27. PMID: 30830835

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ATU_RS20360 (Tae4)
Coordinates (Strand)	1480980..1481480 (+)
NCBI ID	493359403
RefSeq	NZ_CP033035
Uniprot ID	Q7CUP8_AGRFC, A0A083ZID4_RHIRD
KEGG ID	atu:Atu4347
PDB ID	6IJF

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
NnrS	PF05940.14	5.4e-45	33..166

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-37	SIIMSGSETVLPQGRSRPKGGIPRGLARTGPVIFSYG
Transmembrane helix	38-60	FRPFFLGGALWAIVAMVLWIAAL
Outside	61-74	SGFIDIGGDYGAPN
Transmembrane helix	75-97	WHAHEMLFGFASAVLAGFLLTAV
Inside	98-103	PNWTGR
Transmembrane helix	104-126	LPVSGKPLVWLFALWCAGRLFLL
Outside	127-129	VPD
Transmembrane helix	130-152	QVGVVIAAAVDGLFLPALLAICA
Inside	153-167	REVIAGRKWKDLKVL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9934	-	-

Protein sequence: 167 a.a. .

                                    >EFF01860 NZ_CP033035:1480980-1481480 Type VI Amidase Effector (Tae) [Agrobacterium fabrum]
SIIMSGSETVLPQGRSRPKGGIPRGLARTGPVIFSYGFRPFFLGGALWAIVAMVLWIAALSGFIDIGGDYGAPNWHAHEM
LFGFASAVLAGFLLTAVPNWTGRLPVSGKPLVWLFALWCAGRLFLLVPDQVGVVIAAAVDGLFLPALLAICAREVIAGRK
WKDLKVL

                                

Nucleotide sequence: 501 bp .

                                    >EFF01860 NZ_CP033035:1480980-1481480 Type VI Amidase Effector (Tae) [Agrobacterium fabrum]
AGCATTATAATGAGTGGCAGCGAAACCGTTCTCCCCCAAGGGCGCAGCCGGCCAAAGGGCGGCATTCCAAGAGGGCTGGC
GCGCACCGGCCCGGTTATTTTTTCTTACGGCTTCCGGCCGTTTTTCCTCGGCGGCGCCCTCTGGGCCATCGTGGCGATGG
TGTTGTGGATCGCAGCCCTTTCCGGCTTCATCGATATTGGCGGAGACTATGGCGCGCCGAACTGGCATGCCCACGAGATG
CTGTTCGGTTTCGCTTCGGCGGTGCTGGCCGGTTTTCTTTTGACGGCCGTGCCGAACTGGACGGGGCGGCTGCCGGTGTC
GGGCAAACCGCTGGTCTGGCTGTTTGCGCTCTGGTGCGCCGGCCGGCTTTTCCTGCTTGTGCCGGATCAGGTGGGCGTGG
TCATCGCCGCAGCCGTCGACGGGCTGTTCCTGCCGGCGCTGCTGGCGATCTGCGCGCGCGAGGTTATTGCCGGGCGCAAA
TGGAAGGATCTGAAGGTGCTG

                                