Detailed information of effector
Summary
| Effector ID | EFF01857  |
| Effector type | Tae |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS01242 (Type i5) |
| Strain | Agrobacterium tumefaciens strain 1D1108 |
| Replicon | chromosome |
| Sequence | Protein sequence (167 a.a.); Nucleotide sequence (501 bp) |
| Description | Hcp and Tae were detected in the media fraction of each strain while Hcp was not detected in the media fractions of any of the ΔtssL mutants but could be detected in the media fractions of mutant strains complemented with their cognate tssL gene |
| Reference |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | At1D1108_RS23245 (Tae4) |
| Coordinates (Strand) | 2064623..2065123 (+) |
| NCBI ID | 503526812 |
| RefSeq | NZ_CP032922 |
| Uniprot ID | F0LDA2_AGRFH, A0A1S7T3J8_AGRFH, A0A1S7NCB8_AGRFH |
| KEGG ID | agr:AGROH133_09370 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| Tae4 | PF14113.8 | 1.1e-18 | 43..158 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-166 | MRVNFDTLYKNYPSSDPSHPNYVSQRDLFTEIGWDDFIGNPNYHNTCAIRVSIAFVKSGINIVPASHRIQKGPYAGKGIEVNMRRLANLVKRTSYLGEPEPFTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPLQSSRGS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9791 | - | - |
Protein sequence: 167 a.a. .
>EFF01857 NZ_CP032922:2064623-2065123 Type VI Amidase Effector (Tae) [Agrobacterium tumefaciens]
MRVNFDTLYKNYPSSDPSHPNYVSQRDLFTEIGWDDFIGNPNYHNTCAIRVSIAFVKSGINIVPASHRIQKGPYAGKGIE
VNMRRLANLVKRTSYLGEPEPFTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPL
QSSRGS*
MRVNFDTLYKNYPSSDPSHPNYVSQRDLFTEIGWDDFIGNPNYHNTCAIRVSIAFVKSGINIVPASHRIQKGPYAGKGIE
VNMRRLANLVKRTSYLGEPEPFTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPL
QSSRGS*
Nucleotide sequence: 501 bp .
>EFF01857 NZ_CP032922:2064623-2065123 Type VI Amidase Effector (Tae) [Agrobacterium tumefaciens]
ATGCGCGTTAACTTCGACACTCTCTATAAGAATTATCCGAGCAGCGACCCGTCGCACCCGAACTATGTCAGCCAGCGTGA
TCTTTTCACGGAAATCGGCTGGGATGATTTTATCGGTAACCCGAACTATCACAATACCTGTGCCATCCGCGTTAGCATCG
CTTTCGTGAAATCGGGGATCAACATCGTGCCAGCCTCGCATCGCATCCAGAAAGGTCCCTATGCGGGCAAGGGCATTGAG
GTGAACATGCGCCGCCTCGCAAATCTGGTGAAACGGACGAGCTATCTAGGCGAGCCCGAACCGTTTACCCCCGCGACAGC
GCGTAATGGCATCGGTGCCCGTAACGGTGTTGTGGCGTTCAACAATATCCCTGGTTACACCGGAGGCGGCCATATCGATC
TTGTGCGGGGTGGCTCAGAGGCCACCCAATGCGCGTCGGCCTGCTACTACAATTCGGAAACGATCTGGTTCTGGCCACTT
CAGTCCAGCCGCGGGTCCTGA
ATGCGCGTTAACTTCGACACTCTCTATAAGAATTATCCGAGCAGCGACCCGTCGCACCCGAACTATGTCAGCCAGCGTGA
TCTTTTCACGGAAATCGGCTGGGATGATTTTATCGGTAACCCGAACTATCACAATACCTGTGCCATCCGCGTTAGCATCG
CTTTCGTGAAATCGGGGATCAACATCGTGCCAGCCTCGCATCGCATCCAGAAAGGTCCCTATGCGGGCAAGGGCATTGAG
GTGAACATGCGCCGCCTCGCAAATCTGGTGAAACGGACGAGCTATCTAGGCGAGCCCGAACCGTTTACCCCCGCGACAGC
GCGTAATGGCATCGGTGCCCGTAACGGTGTTGTGGCGTTCAACAATATCCCTGGTTACACCGGAGGCGGCCATATCGATC
TTGTGCGGGGTGGCTCAGAGGCCACCCAATGCGCGTCGGCCTGCTACTACAATTCGGAAACGATCTGGTTCTGGCCACTT
CAGTCCAGCCGCGGGTCCTGA