Detailed information of effector


Summary

Effector ID EFF01853 experimental
Effector type Tde
Cognate immunity protein -
Related T6SS (Type) T6SS01240 experimental (Type i5)
Strain Agrobacterium fabrum strain 1D132
Replicon chromosome
Sequence Protein sequence (279 a.a.); Nucleotide sequence (837 bp)
Description Hcp and Tde1 were detected in the media fraction of each strain while Hcp was not detected in the media fractions of any of the ΔtssL mutants but could be detected in the media fractions of mutant strains complemented with their cognate tssL gene
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) At1D132_RS21410 (Tde1)
Coordinates (Strand) 1455387..1456223 (+)
NCBI ID 499280706
RefSeq NZ_CP033023
Uniprot ID A9CGH1_AGRFC
KEGG ID atu:Atu4350
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Ntox15 PF15604.8 2e-55 100..247
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-278 MSATTTVAGAGGAIAGGWLGRVIGGLAGSLAGPGGTAVGAWIGGQVGAMAGRAAASAIASYMEDANVDADAKTKESEQAKPCVDCGEIDCFSPPEGATPKQVEEFRRQLKEQQDEINRMEPDDLVRNIDKYRQQGRPTDDAANRRQSREDYRTDRTRELEEKYLSKGRNDYKEQAANDVAEEMKKLAATHTLDLVAGGDGSISGLGDKSINSSLGSQWKGRRSEQLRSHAKKAAEQKKKMNAKLEECKPEGGNDNSPDAETPDNGTGKGDGNPDVPVS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.4580 - -


  Protein sequence: 279 a.a.    .

>EFF01853 NZ_CP033023:1455387-1456223 Type VI DNase and RNase Effector (Tde) [Agrobacterium fabrum]
MSATTTVAGAGGAIAGGWLGRVIGGLAGSLAGPGGTAVGAWIGGQVGAMAGRAAASAIASYMEDANVDADAKTKESEQAK
PCVDCGEIDCFSPPEGATPKQVEEFRRQLKEQQDEINRMEPDDLVRNIDKYRQQGRPTDDAANRRQSREDYRTDRTRELE
EKYLSKGRNDYKEQAANDVAEEMKKLAATHTLDLVAGGDGSISGLGDKSINSSLGSQWKGRRSEQLRSHAKKAAEQKKKM
NAKLEECKPEGGNDNSPDAETPDNGTGKGDGNPDVPVS*

  Nucleotide sequence: 837 bp    .

>EFF01853 NZ_CP033023:1455387-1456223 Type VI DNase and RNase Effector (Tde) [Agrobacterium fabrum]
ATGAGTGCGACGACAACTGTCGCCGGAGCGGGCGGTGCTATCGCAGGAGGTTGGCTCGGTCGTGTTATTGGAGGCTTAGC
TGGCAGCCTCGCTGGGCCTGGAGGTACAGCGGTTGGAGCATGGATAGGTGGGCAAGTCGGCGCTATGGCGGGTAGAGCGG
CTGCCTCCGCAATCGCCTCTTATATGGAAGATGCGAATGTAGACGCTGACGCCAAGACCAAGGAGAGCGAACAGGCCAAA
CCTTGCGTTGATTGTGGGGAAATCGATTGCTTTAGTCCGCCTGAGGGAGCTACGCCTAAGCAAGTTGAAGAGTTTAGGCG
TCAGTTGAAGGAGCAACAAGACGAAATAAACCGCATGGAGCCTGATGATCTGGTCAGAAATATCGACAAATATAGACAGC
AGGGGAGGCCGACTGATGATGCAGCAAACCGTCGGCAGAGTAGAGAAGATTATCGCACCGACCGCACACGGGAACTGGAA
GAAAAGTATTTATCGAAAGGGCGCAATGACTATAAGGAACAGGCTGCGAATGATGTGGCAGAAGAGATGAAGAAGTTGGC
CGCAACCCATACCTTGGATTTGGTTGCGGGGGGCGACGGATCAATTTCTGGCTTGGGCGACAAGAGTATCAATAGTTCTT
TAGGGTCTCAATGGAAGGGAAGAAGATCGGAGCAATTGCGCTCTCATGCAAAAAAAGCGGCTGAGCAAAAGAAAAAAATG
AACGCTAAACTCGAAGAATGTAAACCCGAAGGCGGCAATGATAATTCTCCCGATGCTGAAACTCCCGACAATGGTACAGG
TAAGGGGGACGGCAATCCTGACGTCCCGGTGTCTTGA