Detailed information of effector


Summary

Effector ID EFF01852 experimental
Effector type Tae
Cognate immunity protein -
Related T6SS (Type) T6SS01240 experimental (Type i5)
Strain Agrobacterium fabrum strain 1D132
Replicon chromosome
Sequence Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Description Hcp and Tae were detected in the media fraction of each strain while Hcp was not detected in the media fractions of any of the ΔtssL mutants but could be detected in the media fractions of mutant strains complemented with their cognate tssL gene
Reference
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) At1D132_RS21395 (Tae4)
Coordinates (Strand) 1451466..1451966 (+)
NCBI ID 493359403
RefSeq NZ_CP033023
Uniprot ID Q7CUP8_AGRFC, A0A083ZID4_RHIRD
KEGG ID atu:Atu4347
PDB ID 6IJF
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Tae4 PF14113.8 7.8e-19 43..158
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-166 MRVNFDTLYSNYPSSDPSHPNYLSQRDLFTEIGWESFIGNPNYHNTCAIRVSIAFVKSGINIVPSSHRIQKGPYAGKGIEVNMRRLATLMKRTSYLGEPDPYTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPLQASRGS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9829 - -


  Protein sequence: 167 a.a.    .

>EFF01852 NZ_CP033023:1451466-1451966 Type VI Amidase Effector (Tae) [Agrobacterium fabrum]
MRVNFDTLYSNYPSSDPSHPNYLSQRDLFTEIGWESFIGNPNYHNTCAIRVSIAFVKSGINIVPSSHRIQKGPYAGKGIE
VNMRRLATLMKRTSYLGEPDPYTPATARNGIGARNGVVAFNNIPGYTGGGHIDLVRGGSEATQCASACYYNSETIWFWPL
QASRGS*

  Nucleotide sequence: 501 bp    .

>EFF01852 NZ_CP033023:1451466-1451966 Type VI Amidase Effector (Tae) [Agrobacterium fabrum]
ATGCGCGTTAACTTTGACACCCTCTATAGCAATTATCCGAGCAGCGATCCGTCGCATCCGAATTATCTCAGCCAGCGTGA
TCTGTTCACGGAAATCGGCTGGGAGAGCTTCATCGGCAATCCGAACTATCATAATACCTGCGCCATCCGCGTCAGCATCG
CTTTCGTGAAGTCGGGCATCAACATCGTGCCCTCTTCGCACCGCATCCAGAAGGGCCCCTATGCCGGAAAGGGCATCGAG
GTGAACATGCGCCGCCTTGCCACCCTGATGAAGCGGACGAGCTATCTCGGCGAGCCCGATCCTTACACGCCCGCCACCGC
CCGCAACGGCATCGGCGCGCGCAACGGTGTCGTGGCCTTCAACAATATTCCCGGCTACACCGGTGGCGGTCATATCGATC
TCGTGCGCGGCGGGTCGGAAGCCACCCAATGCGCGTCGGCCTGCTACTACAATTCGGAAACGATCTGGTTCTGGCCGCTG
CAGGCCAGCCGCGGGTCCTGA