Detailed information of effector
Summary
| Effector ID | EFF01850  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS01112 (Type i1) |
| Strain | Vibrio cholerae strain A1552 |
| Replicon | chromosome |
| Sequence | Protein sequence (173 a.a.); Nucleotide sequence (519 bp) |
| Description | hcp |
| Reference | [1] Ishikawa T, Sabharwal D, Bröms J, et al. Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 2012 Feb;80(2):575-84. doi: 10.1128/IAI.05510-11. Epub 2011 Nov 14. PMID: 22083711 [2] Ishikawa T, Rompikuntal PK, Lindmark B, et al. Quorum sensing regulation of the two hcp alleles in Vibrio cholerae O1 strains. PLoS One. 2009 Aug 24;4(8):e6734. doi: 10.1371/journal.pone.0006734. PMID: 19701456 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | VCA1552_RS05805 (hcp) |
| Coordinates (Strand) | 1238150..1238668 (+) |
| NCBI ID | 447065691 |
| RefSeq | NZ_CP024867 |
| Uniprot ID | H9L4Q3_VIBCH, A0A0X1J838_VIBCO, D7HBV7_VIBCL, A0A0K9V0F0_VIBCL, A0A0H3PYB9_VIBCL, C3LMB0_VIBCM, P72350_VIBCL |
| KEGG ID | vcq:EN18_03855, vcz:VAB027_2088, vcx:VAA049_1881, vch:VC1415, vcm:VCM66_1370, vcm:VCM66_A0016, vch:VCA0017, vcz:VAB027_1005, vcq:EN18_10295 |
| PDB ID | 5MXN, 5OJQ |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 5.3e-35 | 6..155 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-172 | MPTPCYISIEGQTQGLITAGACTADSIGDSFVEGHEDEMLVQQFDHVVTVPTDPQSGQPSGQRVHKPFKFTVALNKAVPLLYNALSSGEKLKTVELKWYRTSIEGKQENFFTTKLENASIVDIHCEMPHCQDPAKSDFTQNVTVSLSYRKITWDHVNAGTSGSDDWRKPIEA |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.7783 | - | - |
Protein sequence: 173 a.a. .
>EFF01850 NZ_CP024867:1238150-1238668 (Unknown) [Vibrio cholerae]
MPTPCYISIEGQTQGLITAGACTADSIGDSFVEGHEDEMLVQQFDHVVTVPTDPQSGQPSGQRVHKPFKFTVALNKAVPL
LYNALSSGEKLKTVELKWYRTSIEGKQENFFTTKLENASIVDIHCEMPHCQDPAKSDFTQNVTVSLSYRKITWDHVNAGT
SGSDDWRKPIEA*
MPTPCYISIEGQTQGLITAGACTADSIGDSFVEGHEDEMLVQQFDHVVTVPTDPQSGQPSGQRVHKPFKFTVALNKAVPL
LYNALSSGEKLKTVELKWYRTSIEGKQENFFTTKLENASIVDIHCEMPHCQDPAKSDFTQNVTVSLSYRKITWDHVNAGT
SGSDDWRKPIEA*
Nucleotide sequence: 519 bp .
>EFF01850 NZ_CP024867:1238150-1238668 (Unknown) [Vibrio cholerae]
ATGCCAACTCCATGTTATATCTCTATCGAAGGCCAAACTCAGGGTCTTATCACTGCAGGTGCATGTACTGCTGACTCTAT
CGGCGATTCATTCGTTGAAGGCCACGAAGATGAGATGCTGGTTCAGCAGTTTGACCACGTTGTGACTGTACCGACTGACC
CACAATCTGGTCAGCCTTCAGGCCAACGTGTGCACAAGCCATTCAAATTCACTGTGGCGCTGAACAAAGCGGTTCCTCTG
CTGTACAACGCGCTCTCTTCTGGTGAGAAGCTGAAAACCGTTGAGCTGAAGTGGTACCGTACCTCTATCGAAGGTAAACA
AGAGAACTTCTTCACCACTAAGCTGGAAAACGCGTCTATCGTCGACATCCACTGTGAAATGCCACACTGCCAAGACCCAG
CAAAATCTGACTTTACTCAGAATGTGACTGTGTCTCTGTCTTACCGCAAAATCACTTGGGACCACGTTAACGCGGGCACC
TCAGGCTCTGATGACTGGCGTAAGCCAATCGAAGCGTAA
ATGCCAACTCCATGTTATATCTCTATCGAAGGCCAAACTCAGGGTCTTATCACTGCAGGTGCATGTACTGCTGACTCTAT
CGGCGATTCATTCGTTGAAGGCCACGAAGATGAGATGCTGGTTCAGCAGTTTGACCACGTTGTGACTGTACCGACTGACC
CACAATCTGGTCAGCCTTCAGGCCAACGTGTGCACAAGCCATTCAAATTCACTGTGGCGCTGAACAAAGCGGTTCCTCTG
CTGTACAACGCGCTCTCTTCTGGTGAGAAGCTGAAAACCGTTGAGCTGAAGTGGTACCGTACCTCTATCGAAGGTAAACA
AGAGAACTTCTTCACCACTAAGCTGGAAAACGCGTCTATCGTCGACATCCACTGTGAAATGCCACACTGCCAAGACCCAG
CAAAATCTGACTTTACTCAGAATGTGACTGTGTCTCTGTCTTACCGCAAAATCACTTGGGACCACGTTAACGCGGGCACC
TCAGGCTCTGATGACTGGCGTAAGCCAATCGAAGCGTAA