SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01846
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS01103 (Type i5)
Strain	Agrobacterium tumefaciens strain 1D1609
Replicon	chromosome
Sequence	Protein sequence (756 a.a.); Nucleotide sequence (2268 bp)
Description	VgrG
Reference	[1] Santos MNM, Cho ST, Wu CF, et al. Redundancy and Specificity of Type VI Secretion vgrG Loci in Antibacterial Activity of Agrobacterium tumefaciens 1D1609 Strain. Front Microbiol. 2020 Jan 14;10:3004. doi: 10.3389/fmicb.2019.03004. eCollection 2019. PMID: 31993035

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	At1D1609_RS26290
Coordinates (Strand)	2295823..2298090 (+)
NCBI ID	1352522879
RefSeq	NZ_CP026925
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	3.7e-73	36..347
Phage_base_V	PF04717.14	2.9e-12	401..468

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-755	MNDQPSSSDFIQASRVLKVKSTLGEDQLLPERLTVEEGVSQLFDIRLTVRAKKEAVKPEELIGRLVDVSVEVQQGDGEEGSGVRRPFNGLVTELHEGPPITRGMRSYAMTIRPQMWLLSRRSDCRIWMDKTAVEVVETLFSEHGIPAPDTSGIISPPPAQHYSVQWNETDLDYLTRRFEEDGLFYWFSHEDGSHKLHVADSASGWLGPSPAAQGEGTVRLAQGSSDRNHINDWARRFSYVSGQRAGADWNFETPGMVPGTMTPSLVQMPDATKRELYEYPARIKTVEEAERAQKLRTQSIEADHDRVFGSSTSRILEAGRRFTPYEVAHPEHAYEEHVIIRASHNIVDLSYETNGNEPEYRNHFEAIPARVPLTPHRSTKRPRIEGTQVAIVAGPEGEEIHPDQYGRIKLWFPWDRKAKKDGTDTCWVRVSQAWGGGTWGAQVIPRIGMEVMVAFVDGDPDKPLVIGVVNNPANSVPYDLPANKTRMVLRSNSHKGDGFNEITFEDEAGKENQFFHAQKDQTTRVLNDRTKRIDRHEVASVGGNRAVEVSGNQKHEIGGSVNTVVGGTGPMAMMAMAGVQALSGQTAGLLSQAAQIAGGGGPGVAAFATTLASSALGFLGAGGLSSREGVVSGPSPRADAGTALAGSGSGVGSDASGLFPLPGIMNTIIGSFQSTSVGIAKTEQVGVSKVTNVGQTSMENVGKAKKLTVGEEYVIEVGKSSMTMKKDGTIIIKGVTFHFEAEGSFQQIGKVIDLN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9923	-	-

Protein sequence: 756 a.a. .

                                    >EFF01846 NZ_CP026925:2295823-2298090  (Unknown) [Agrobacterium tumefaciens]
MNDQPSSSDFIQASRVLKVKSTLGEDQLLPERLTVEEGVSQLFDIRLTVRAKKEAVKPEELIGRLVDVSVEVQQGDGEEG
SGVRRPFNGLVTELHEGPPITRGMRSYAMTIRPQMWLLSRRSDCRIWMDKTAVEVVETLFSEHGIPAPDTSGIISPPPAQ
HYSVQWNETDLDYLTRRFEEDGLFYWFSHEDGSHKLHVADSASGWLGPSPAAQGEGTVRLAQGSSDRNHINDWARRFSYV
SGQRAGADWNFETPGMVPGTMTPSLVQMPDATKRELYEYPARIKTVEEAERAQKLRTQSIEADHDRVFGSSTSRILEAGR
RFTPYEVAHPEHAYEEHVIIRASHNIVDLSYETNGNEPEYRNHFEAIPARVPLTPHRSTKRPRIEGTQVAIVAGPEGEEI
HPDQYGRIKLWFPWDRKAKKDGTDTCWVRVSQAWGGGTWGAQVIPRIGMEVMVAFVDGDPDKPLVIGVVNNPANSVPYDL
PANKTRMVLRSNSHKGDGFNEITFEDEAGKENQFFHAQKDQTTRVLNDRTKRIDRHEVASVGGNRAVEVSGNQKHEIGGS
VNTVVGGTGPMAMMAMAGVQALSGQTAGLLSQAAQIAGGGGPGVAAFATTLASSALGFLGAGGLSSREGVVSGPSPRADA
GTALAGSGSGVGSDASGLFPLPGIMNTIIGSFQSTSVGIAKTEQVGVSKVTNVGQTSMENVGKAKKLTVGEEYVIEVGKS
SMTMKKDGTIIIKGVTFHFEAEGSFQQIGKVIDLN*

                                

Nucleotide sequence: 2268 bp .

                                    >EFF01846 NZ_CP026925:2295823-2298090  (Unknown) [Agrobacterium tumefaciens]
ATGAATGACCAGCCCTCATCCTCTGACTTCATTCAGGCGAGTCGTGTGCTCAAGGTCAAGTCGACTTTGGGCGAGGATCA
GCTTCTTCCGGAGCGTCTGACGGTCGAGGAAGGTGTATCGCAACTCTTCGACATCCGCCTGACGGTGCGGGCCAAGAAAG
AAGCGGTCAAGCCCGAAGAACTGATCGGACGGCTGGTCGATGTTTCAGTCGAGGTGCAACAGGGCGATGGCGAAGAGGGC
AGCGGCGTGCGCCGCCCCTTCAACGGCCTGGTTACCGAGTTGCATGAGGGTCCGCCGATCACCCGCGGCATGCGCTCCTA
TGCCATGACCATCCGCCCGCAAATGTGGCTTCTGTCACGCCGGTCCGATTGCCGCATCTGGATGGACAAGACGGCGGTGG
AGGTCGTCGAGACGCTGTTTTCCGAACACGGTATTCCCGCACCCGATACATCGGGCATCATCTCGCCGCCGCCCGCACAG
CATTACAGCGTGCAATGGAACGAGACGGATCTCGATTATCTCACCCGCCGTTTTGAAGAGGACGGCCTGTTCTACTGGTT
CAGCCATGAGGATGGTTCCCACAAGCTGCATGTGGCCGATAGCGCCAGCGGCTGGCTTGGGCCGTCGCCGGCAGCCCAGG
GGGAGGGGACGGTGCGCCTGGCGCAGGGGTCTTCGGACCGTAACCACATCAACGACTGGGCGCGGCGTTTTTCCTATGTT
TCGGGCCAGCGCGCTGGCGCGGACTGGAATTTCGAGACGCCGGGCATGGTGCCGGGCACGATGACACCGTCGCTGGTGCA
GATGCCGGATGCGACGAAGCGCGAGCTTTACGAATATCCGGCCCGCATCAAGACGGTGGAAGAGGCCGAGCGCGCGCAGA
AACTGCGCACCCAGTCCATCGAAGCCGATCACGACCGGGTTTTCGGTTCGTCGACCTCGCGCATTCTGGAGGCCGGCCGC
CGTTTCACGCCTTACGAGGTCGCCCATCCCGAACACGCCTATGAAGAACATGTGATCATCAGGGCAAGCCACAATATTGT
CGATCTCTCTTATGAGACCAATGGCAACGAGCCGGAATATCGCAATCACTTCGAGGCGATCCCCGCCCGTGTGCCGCTGA
CGCCGCACCGGTCAACAAAACGCCCGCGCATCGAAGGGACGCAGGTGGCGATCGTCGCCGGACCTGAGGGCGAGGAAATC
CATCCTGACCAATATGGCCGCATCAAGCTGTGGTTCCCGTGGGACCGCAAGGCGAAGAAGGATGGCACGGATACGTGCTG
GGTTCGTGTCAGCCAGGCATGGGGCGGCGGCACTTGGGGTGCGCAGGTCATTCCGCGCATCGGCATGGAAGTCATGGTCG
CCTTTGTCGATGGCGACCCGGATAAGCCGCTGGTCATCGGCGTGGTCAACAATCCGGCCAATTCCGTGCCTTACGACCTG
CCCGCCAACAAGACACGTATGGTGCTGCGCTCCAACAGCCACAAGGGCGACGGTTTCAACGAAATCACCTTCGAGGATGA
GGCCGGCAAGGAGAACCAGTTCTTCCACGCCCAGAAGGACCAGACGACGCGGGTTCTGAATGACCGGACCAAGCGCATCG
ACCGGCATGAGGTCGCCTCGGTCGGCGGCAACCGCGCGGTGGAAGTTTCCGGCAACCAGAAACACGAGATCGGCGGCTCG
GTAAACACCGTCGTCGGCGGCACGGGACCGATGGCGATGATGGCCATGGCCGGCGTACAGGCGCTCTCCGGGCAGACGGC
GGGCCTGCTTTCGCAGGCCGCCCAGATCGCCGGCGGCGGTGGCCCGGGCGTCGCGGCCTTCGCCACCACGCTCGCCTCAT
CCGCACTCGGCTTCCTCGGAGCCGGCGGTCTTTCATCGCGTGAGGGGGTCGTTTCCGGCCCGAGCCCGCGCGCGGATGCG
GGTACGGCGCTCGCCGGCTCCGGCTCGGGCGTCGGCTCCGATGCTTCAGGTCTCTTTCCGCTACCGGGCATCATGAACAC
GATCATCGGTTCGTTTCAGTCCACATCTGTTGGCATCGCAAAAACCGAGCAGGTCGGGGTCAGCAAGGTCACAAATGTCG
GCCAGACGTCGATGGAAAATGTCGGCAAGGCGAAGAAACTCACTGTGGGAGAGGAATATGTCATCGAAGTCGGCAAGTCG
TCCATGACGATGAAAAAGGACGGCACGATCATCATTAAGGGCGTGACGTTTCATTTCGAGGCTGAAGGTTCGTTTCAGCA
GATCGGCAAAGTCATAGATCTCAACTGA

                                