SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01844
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS01103 (Type i5)
Strain	Agrobacterium tumefaciens strain 1D1609
Replicon	chromosome
Sequence	Protein sequence (755 a.a.); Nucleotide sequence (2265 bp)
Description	VgrG
Reference	[1] Santos MNM, Cho ST, Wu CF, et al. Redundancy and Specificity of Type VI Secretion vgrG Loci in Antibacterial Activity of Agrobacterium tumefaciens 1D1609 Strain. Front Microbiol. 2020 Jan 14;10:3004. doi: 10.3389/fmicb.2019.03004. eCollection 2019. PMID: 31993035

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	At1D1609_RS22460
Coordinates (Strand)	1475318..1477582 (+)
NCBI ID	1352522504
RefSeq	NZ_CP026925
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	8.4e-74	36..346
Phage_base_V	PF04717.14	2.9e-12	400..467

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-754	MNDQPSSSDFIQASRVLKVKSPLGENQLLPERLSVEEGVSQLFDIRLTVRAQKEAVKPEELIGRLVDISIEISQGEGDGGGVRRPFNGLVTELHEGPPITRGMRSYALTIRPQMWLLSRRSDCRIWMDKTAVEVVETLFSEHGIPAPDTSGIISPPPAQHYSVQWNETDLDYLTRRFEEDGLFYWFSHEDGSHKLHVADSASGWLGPSPAAQGEGTVRLAQGSSDRNHINDWARRFSYVSGQRAGADWNFETPGMVPGTMTPSLVQMPDATKRELYEYPARIKTVEEAERAQKLRTQSIEADHDRVFGSSTSRILEAGRRFTPYEVAHPEHAYEEHVIIRASHNIVDLSYETNGNEPEYRNHFEAIPARVPLTPHRSTKRPRIEGTQVAIVAGPEGEEIHPDQYGRIKLWFPWDRKAKKDGTDTCWVRVSQAWGGGTWGAQVIPRIGMEVMVAFVDGDPDKPLVIGVVNNPANSVPYDLPANKTRMVLRSNSHKGDGFNEITFEDEAGKENQFFHAQKDQTTRVLNDRTKRIDRHEVASVGGNRAVEVSGNQKHEIGGSVNTVVGGTGPMAMMAMAGVQALSGQTAGLLSQAAQIAGGGGPGVAAFATTLASSALGFLGAGGLSSREGVVSGPSPRADAGTALAGSGSGVGSDASGLFPLPGIMNTIVSTFKSDSVGVARAEQIGVSKVTNVGQTSLESVGKFKKIAVGEEFVIECGDSKFIMKANGEVIILGKTFNFVATDHFQLRGKPIDLN

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9941	-	-

Protein sequence: 755 a.a. .

                                    >EFF01844 NZ_CP026925:1475318-1477582  (Unknown) [Agrobacterium tumefaciens]
MNDQPSSSDFIQASRVLKVKSPLGENQLLPERLSVEEGVSQLFDIRLTVRAQKEAVKPEELIGRLVDISIEISQGEGDGG
GVRRPFNGLVTELHEGPPITRGMRSYALTIRPQMWLLSRRSDCRIWMDKTAVEVVETLFSEHGIPAPDTSGIISPPPAQH
YSVQWNETDLDYLTRRFEEDGLFYWFSHEDGSHKLHVADSASGWLGPSPAAQGEGTVRLAQGSSDRNHINDWARRFSYVS
GQRAGADWNFETPGMVPGTMTPSLVQMPDATKRELYEYPARIKTVEEAERAQKLRTQSIEADHDRVFGSSTSRILEAGRR
FTPYEVAHPEHAYEEHVIIRASHNIVDLSYETNGNEPEYRNHFEAIPARVPLTPHRSTKRPRIEGTQVAIVAGPEGEEIH
PDQYGRIKLWFPWDRKAKKDGTDTCWVRVSQAWGGGTWGAQVIPRIGMEVMVAFVDGDPDKPLVIGVVNNPANSVPYDLP
ANKTRMVLRSNSHKGDGFNEITFEDEAGKENQFFHAQKDQTTRVLNDRTKRIDRHEVASVGGNRAVEVSGNQKHEIGGSV
NTVVGGTGPMAMMAMAGVQALSGQTAGLLSQAAQIAGGGGPGVAAFATTLASSALGFLGAGGLSSREGVVSGPSPRADAG
TALAGSGSGVGSDASGLFPLPGIMNTIVSTFKSDSVGVARAEQIGVSKVTNVGQTSLESVGKFKKIAVGEEFVIECGDSK
FIMKANGEVIILGKTFNFVATDHFQLRGKPIDLN*

                                

Nucleotide sequence: 2265 bp .

                                    >EFF01844 NZ_CP026925:1475318-1477582  (Unknown) [Agrobacterium tumefaciens]
ATGAATGACCAGCCCTCATCCTCTGACTTCATTCAGGCGAGTCGTGTGCTCAAGGTCAAGTCGCCTTTGGGCGAGAATCA
GCTTCTGCCAGAGCGCCTGTCCGTCGAGGAGGGCGTATCGCAGCTCTTCGACATTCGCCTGACGGTGCGTGCCCAGAAGG
AAGCGGTCAAGCCCGAAGAGCTGATCGGACGGCTGGTCGATATCTCCATCGAGATCAGCCAGGGCGAGGGCGATGGCGGC
GGTGTTCGCCGCCCCTTTAACGGCCTGGTGACGGAGCTGCATGAAGGCCCGCCGATCACCCGCGGCATGCGCTCCTATGC
GCTGACCATCCGCCCGCAAATGTGGCTTCTGTCGCGCCGGTCGGATTGCCGTATCTGGATGGACAAGACGGCGGTGGAGG
TCGTCGAGACGCTGTTTTCCGAACACGGCATTCCCGCTCCCGATACATCGGGCATCATCTCGCCGCCGCCCGCACAGCAT
TACAGCGTGCAATGGAACGAGACGGATCTCGATTATCTCACCCGCCGTTTTGAAGAGGACGGCCTGTTCTACTGGTTCAG
CCATGAGGATGGTTCTCACAAGCTGCATGTGGCCGATAGCGCCAGCGGCTGGCTTGGGCCGTCGCCGGCAGCCCAGGGGG
AGGGGACGGTGCGTCTGGCGCAGGGGTCTTCGGATCGTAACCACATCAACGACTGGGCGCGGCGTTTTTCCTATGTTTCG
GGCCAGCGCGCTGGCGCGGACTGGAATTTCGAGACGCCGGGCATGGTGCCGGGCACGATGACACCGTCGCTGGTGCAGAT
GCCGGATGCGACGAAGCGCGAGCTTTACGAATATCCGGCCCGCATCAAGACGGTGGAAGAGGCCGAGCGCGCGCAGAAAC
TGCGCACCCAGTCCATCGAAGCCGATCACGACCGGGTTTTCGGTTCGTCGACCTCGCGCATTCTGGAGGCCGGCCGCCGT
TTCACGCCTTACGAGGTCGCCCATCCCGAACACGCCTATGAAGAACATGTGATCATCAGGGCAAGCCACAATATTGTCGA
TCTCTCTTATGAGACCAATGGCAACGAGCCGGAATATCGCAATCACTTCGAGGCGATCCCCGCCCGTGTGCCGCTGACGC
CGCACCGGTCAACAAAACGCCCGCGCATCGAAGGGACGCAGGTGGCGATCGTCGCCGGACCTGAAGGCGAGGAAATCCAT
CCTGACCAATATGGCCGCATCAAGCTGTGGTTCCCGTGGGACCGCAAGGCGAAGAAGGATGGCACGGATACGTGCTGGGT
TCGTGTGAGCCAGGCATGGGGCGGTGGCACATGGGGTGCGCAGGTCATTCCGCGCATCGGCATGGAGGTGATGGTCGCCT
TTGTCGATGGAGACCCGGATAAGCCGCTGGTCATCGGCGTGGTCAACAATCCGGCCAATTCCGTGCCTTACGACCTGCCG
GCCAACAAGACGCGCATGGTGCTGCGCTCCAACAGCCACAAGGGCGACGGTTTCAACGAAATCACCTTCGAGGATGAGGC
CGGCAAGGAGAACCAGTTTTTCCACGCCCAGAAGGACCAGACGACGCGGGTTCTGAATGACCGGACCAAGCGCATCGATC
GGCATGAGGTCGCCTCGGTCGGCGGCAACCGCGCGGTGGAAGTTTCCGGCAACCAGAAACACGAGATCGGCGGCTCGGTA
AACACCGTCGTCGGCGGAACGGGGCCGATGGCGATGATGGCCATGGCCGGCGTGCAGGCGCTCTCCGGGCAGACGGCGGG
GCTGCTTTCGCAGGCCGCCCAGATCGCCGGCGGCGGTGGCCCGGGTGTCGCGGCCTTCGCCACCACGCTCGCCTCATCCG
CACTCGGCTTCCTCGGCGCAGGCGGTCTTTCATCGCGTGAGGGGGTCGTTTCCGGCCCGAGCCCACGTGCGGATGCGGGC
ACGGCGCTCGCCGGCTCCGGCTCGGGCGTCGGTTCCGATGCTTCGGGATTGTTTCCGCTGCCGGGCATCATGAACACGAT
CGTCAGCACGTTCAAATCCGACAGCGTCGGGGTGGCGCGGGCAGAACAGATCGGGGTGAGCAAGGTTACGAATGTCGGCC
AGACGTCGCTGGAAAGCGTCGGAAAGTTCAAGAAGATTGCAGTCGGTGAAGAGTTCGTCATCGAATGCGGCGATTCCAAA
TTCATCATGAAGGCGAATGGCGAGGTCATCATTCTTGGCAAGACCTTCAACTTCGTGGCGACGGATCATTTTCAGCTTCG
CGGCAAGCCGATCGATCTGAACTGA

                                