SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01812
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS01032 (Type i4b)
Strain	Acinetobacter baumannii AB307-0294
Replicon	chromosome
Sequence	Protein sequence (303 a.a.); Nucleotide sequence (909 bp)
Description	-
Reference	[1] Fitzsimons TC, Lewis JM, Wright A, et al. Identification of Novel Acinetobacter baumannii Type VI Secretion System Antibacterial Effector and Immunity Pairs. Infect Immun. 2018 Jul 23;86(8). pii: e00297-18. doi: 10.1128/IAI.00297-18. Print 2018 Aug. PMID: 29735524

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ABBFA_02229 (Hcp)
Coordinates (Strand)	2404379..2405287 (-)
NCBI ID	1281363083
RefSeq	NZ_CP001172
Uniprot ID	A0A0D5YK54_ACIBA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Sel1	PF08238.14	2.6e-11	78..112
Sel1	PF08238.14	1.7e-10	43..77
Sel1	PF08238.14	9.6e-10	150..184
Sel1	PF08238.14	1.2e-09	115..149
Sel1	PF08238.14	3.7e-09	259..293
Sel1	PF08238.14	1.9e-08	224..255
Sel1	PF08238.14	8.3e-08	186..221

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-302	MFFILRKKLQKIGIILVAINLLACGHQPDFKDITHLAESGDAGAQAKLGELYVEGQVVPQDYKKAFEWYSKAANQGNAEAQNNLGAMYALGQGVEQNYKKAFEWYSKAAEQGEVKAQNNLGAYYANGDGGVKNYQKAFEWFSKAAAQDNAEAKYYLGILYEEGYGVTQDYKKAFEWYSKAAAQNYADAQNNLAALYAQGKGVELNNKKAFELYSKAAEQGNEKAQNNLGAIYALGIGVNQDYKKAFEWYSKAAQQENDEAQFTVGMMYYKGEGVQQNNELAEKWLRKAAENGNKDALSLFEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9978	pos: 23-24. LLA-CG.	MFFILRKKLQKIGIILVAINLLA

Protein sequence: 303 a.a. .

                                    >EFF01812 NZ_CP001172:c2405287-2404379  (Unknown) [Acinetobacter baumannii AB307-0294]
MFFILRKKLQKIGIILVAINLLACGHQPDFKDITHLAESGDAGAQAKLGELYVEGQVVPQDYKKAFEWYSKAANQGNAEA
QNNLGAMYALGQGVEQNYKKAFEWYSKAAEQGEVKAQNNLGAYYANGDGGVKNYQKAFEWFSKAAAQDNAEAKYYLGILY
EEGYGVTQDYKKAFEWYSKAAAQNYADAQNNLAALYAQGKGVELNNKKAFELYSKAAEQGNEKAQNNLGAIYALGIGVNQ
DYKKAFEWYSKAAQQENDEAQFTVGMMYYKGEGVQQNNELAEKWLRKAAENGNKDALSLFEE*

                                

Nucleotide sequence: 909 bp .

                                    >EFF01812 NZ_CP001172:c2405287-2404379  (Unknown) [Acinetobacter baumannii AB307-0294]
ATGTTTTTTATTTTAAGAAAAAAACTTCAAAAAATCGGCATTATTTTAGTAGCAATCAACTTATTAGCTTGTGGCCATCA
ACCAGATTTTAAAGATATTACGCATTTAGCAGAAAGTGGTGATGCAGGAGCTCAAGCAAAACTTGGCGAATTATATGTAG
AGGGACAGGTTGTTCCTCAAGATTATAAAAAGGCCTTTGAATGGTATAGCAAAGCTGCTAATCAAGGAAATGCAGAGGCA
CAGAATAATTTGGGAGCAATGTATGCTCTTGGACAAGGGGTAGAGCAAAATTATAAAAAAGCATTCGAATGGTATAGTAA
AGCTGCTGAACAAGGAGAAGTGAAAGCACAAAATAATTTAGGTGCATATTATGCAAATGGTGATGGTGGAGTTAAAAACT
ATCAGAAAGCCTTTGAGTGGTTTAGCAAAGCTGCTGCACAAGATAATGCCGAAGCTAAATACTATTTGGGCATACTTTAT
GAAGAAGGTTATGGGGTAACCCAAGATTATAAAAAGGCCTTTGAATGGTATAGCAAAGCTGCTGCACAAAATTATGCCGA
TGCGCAAAATAATCTTGCAGCACTTTATGCACAAGGTAAGGGCGTAGAGCTAAATAATAAAAAAGCCTTTGAGTTATATA
GCAAAGCGGCTGAGCAAGGAAATGAAAAAGCACAAAATAACTTGGGGGCGATATATGCTCTTGGGATAGGTGTAAATCAA
GATTATAAGAAAGCATTCGAATGGTATAGCAAAGCTGCTCAACAGGAAAATGACGAAGCACAATTTACTGTAGGGATGAT
GTACTATAAAGGTGAAGGCGTACAACAAAATAATGAATTAGCCGAAAAGTGGCTAAGAAAAGCAGCAGAAAATGGCAATA
AAGATGCTTTATCGCTATTTGAAGAATAG

                                