SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01802
Effector type	Tse
Cognate immunity protein	-
Related T6SS (Type)	T6SS00061 (Type i4b)
Strain	Acinetobacter baylyi ADP1
Replicon	chromosome
Sequence	Protein sequence (387 a.a.); Nucleotide sequence (1160 bp)
Description	Tse
Reference	[1] Ringel PD, Hu D, Basler M. The Role of Type VI Secretion System Effectors in Target Cell Lysis and Subsequent Horizontal Gene Transfer. Cell Rep. 2017 Dec 26;21(13):3927-3940. doi: 10.1016/j.celrep.2017.12.020. PMID: 29281838

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	ACIAD_RS08265
Coordinates (Strand)	1799607..1800766 (-)
NCBI ID	1268821373
RefSeq	NC_005966
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
rve	PF00665.28	3.1e-26	213..311
rve_2	PF13333.8	1.2e-13	318..372
HTH_Tnp_1	PF01527.22	4.7e-10	3..77
HTH_21	PF13276.8	3.9e-07	139..189

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-386	MAKRFSPEFKQQAIDYALSNSHEPIATIAQKLGVGYSTLDKWIREANPVGSSKRQLSPEQQRILELEKEVKQLREANDNLKKSACVLSDRSCQEKYTVIQDLDVNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDLKAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRSKIARKYKHTTDSNHRLPTAPNLLDRQFTVNEPNKIWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVMVHSDQGSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAVLFDYIEIYYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9948	-	-

Protein sequence: 387 a.a. .

                                    >EFF01802 NC_005966:c1800766-1799607 [Acinetobacter]
MAKRFSPEFKQQAIDYALSNSHEPIATIAQKLGVGYSTLDKWIREANPVGSSKRQLSPEQQRILELEKEV
KQLREANDNLKKSACVLSDRSCQEKYTVIQDLDVNEVTVSSACKCLGVSTSGYYAWRKRQTNLAQKYNDL
KAVYWQHHARLGAPSLVHDMHDLGYSMSERTVGRMLKKLGLRSKIARKYKHTTDSNHRLPTAPNLLDRQF
TVNEPNKIWTTDITYIRTKQGWLYLCVMLDLFSRRIVGWQTSHRIDRQLVCDAFHYAMARQGYPMGVMVH
SDQGSQYCSRDFRALLLTNNCVQSMSRRGNCWDNAVTESFFHTLKGHMVHGSVFATRKEANAVLFDYIEI
YYNRIRRHSTNGWLSPEAFEQKYFKNLEGFVVHDTV*

                                

Nucleotide sequence: 1160 bp .

                                    >EFF01802 NC_005966:c1800766-1799607 [Acinetobacter]
ATGGCCAAACGTTTTAGTCCTGAATTTAAACAGCAAGCAATTGATTATGCACTTTCAAACTCACACGAGC
CTATAGCTACAATCGCCCAGAAATTAGGTGTGGGTTATTCAACTTTAGACAAATGGATTCGTGAAGCCAA
TCCAGTGGGTTCAAGCAAACGTCAACTTTCACCAGAACAACAGCGGATCTTGGAATTAGAGAAAGAAGTC
AAACAGCTCAGGGAAGCCAATGACATCTTAAAAAAAGCGCATGTGTACTTTCTGACAGATCATGCCAAGA
AAAGTACACGGTAATTCAAGATCTGGATGTGAATGAAGTCACTGTATCTTCTGCCTGTAAATGCCTGGGT
GTCAGCACTTCAGGCTATTATGCCTGGCGAAAACGCCAGACCAATCTAGCGCAGAAATATAATGATTTAA
AAGCCGTGTATTGGCAGCATCATGCACGATTGGGTGCACCTTCATTGGTACATGACATGCATGATTTAGG
TTACAGCATGAGCGAACGAACCGTTGGAAGAATGCTTAAAAAACTTGGTTTACGGAGCAAAATTGCACGT
AAATACAAGCATACGACTGATTCAAACCATCGTTTGCCTACAGCGCCTAACTTGTTGGATCGTCAATTTA
CGGTCAATGAACCCAATAAGATCTGGACAACGGATATTACCTATATCCGTACTAAGCAAGGTTGGCTGTA
TTTATGTGTGATGCTGGATCTATTCAGCCGTCGTATTGTCGGTTGGCAAACCAGCCATCGAATAGACCGT
CAATTGGTGTGTGATGCGTTTCATTATGCAATGGCTCGTCAGGGGTATCCAATGGGTGTCATGGTACATT
CGGACCAAGGCTCACAGTACTGTAGTCGTGATTTTAGGGCGCTATTATTGACGAATAACTGCGTTCAAAG
TATGTCACGCAGGGGAAACTGTTGGGATAATGCAGTGACCGAAAGCTTCTTTCATACATTGAAAGGTCAT
ATGGTCCATGGCAGTGTGTTTGCCACTCGAAAAGAAGCGAATGCTGTCTTGTTTGACTATATTGAGATTT
ATTACAATCGAATCAGACGACATTCTACAAACGGCTGGTTAAGCCCAGAAGCCTTTGAACAGAAATATTT
CAAGAATTTAGAGGGATTTGTTGTCCACGATACTGTCTAG

                                