SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01124
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00838 (Type i1)
Strain	Proteus mirabilis BB2000
Replicon	chromosome
Sequence	Protein sequence (724 a.a.); Nucleotide sequence (2172 bp)
Description	-
Reference	[1] Wenren LM, Sullivan NL, Cardarelli L, et al. Two independent pathways for self-recognition in Proteus mirabilis are linked by type VI-dependent export. mBio. 2013 Jul 23;4(4). pii: e00374-13. doi: 10.1128/mBio.00374-13. PMID: 23882014

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	BB2000_RS14600
Coordinates (Strand)	3276277..3278448 (+)
NCBI ID	529238653
RefSeq	NC_022000
Uniprot ID	UPI00017A2F77
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	7.2e-64	82..374
Phage_base_V	PF04717.14	1.8e-09	432..496

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-723	MSITNSKKYQRLGDKDGNSLSGDKTLTHGGGRTYAQDLALQEQHRQLIEKILSGDMQSGLVFTCQIGGLPEETFQVTQFDLQEGISQLFTLSIQAVSPLSEIDFNDVLGMASSLTVKRDGHILRTVQGILAAAEQGNTDGVKTWYQFVIRPEMWVMTLKQDSRIFQDTTVPHILETLLNESHIKYDKQLYHPEEHLQRAYTTQKRETMYDFWCRLAAEEGINFWFEEGPQLFYSDRHLGMRAGISLTYNPQSDTDITDSTATTWRYVEQLCSDIRVDKDYNQLRPSYPLSHQVTGEVHQQHEIFESYGRFEEDAQGQPFNQIRYEQSQNQRQVGSATTNCIELAPGRIFTLSNHPSARMNTTWQVISISHHGVQPLADNSGGEGTQLSNQLSFVPSTQEWRPPYRFKPTADGDELATVVGPPGEEIYTNEQGAVTVYFHWDRRGKPDHSASCWVRVAQGWNGDGFGFMAIPRVGQEVIISYLNNDIDKPIITGCTYNGRNRSPIDLPKHKTRTTFRTKTHKGDGFNELRFEDKNGDEEIYLHAQRDNNIHINHNKTQYVGHDDSHTVANNRKHEVCHDEFIHIVNEQTVQVDVNQFEQIGKDRITRIDNSWKEVIHASHFQEIGENKQCDIQNDYTLKAAGGIHTHTKIHTLQASETVIIKGKAGSIQLDAQGVTITGKILLKGDVSITGGAPEVVTQLEAAVNEGLPLAEDCRAKAMKKGTA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9802	-	-

Protein sequence: 724 a.a. .

                                    >EFF01124 NC_022000:3276277-3278448  (Unknown) [Proteus mirabilis BB2000]
MSITNSKKYQRLGDKDGNSLSGDKTLTHGGGRTYAQDLALQEQHRQLIEKILSGDMQSGLVFTCQIGGLPEETFQVTQFD
LQEGISQLFTLSIQAVSPLSEIDFNDVLGMASSLTVKRDGHILRTVQGILAAAEQGNTDGVKTWYQFVIRPEMWVMTLKQ
DSRIFQDTTVPHILETLLNESHIKYDKQLYHPEEHLQRAYTTQKRETMYDFWCRLAAEEGINFWFEEGPQLFYSDRHLGM
RAGISLTYNPQSDTDITDSTATTWRYVEQLCSDIRVDKDYNQLRPSYPLSHQVTGEVHQQHEIFESYGRFEEDAQGQPFN
QIRYEQSQNQRQVGSATTNCIELAPGRIFTLSNHPSARMNTTWQVISISHHGVQPLADNSGGEGTQLSNQLSFVPSTQEW
RPPYRFKPTADGDELATVVGPPGEEIYTNEQGAVTVYFHWDRRGKPDHSASCWVRVAQGWNGDGFGFMAIPRVGQEVIIS
YLNNDIDKPIITGCTYNGRNRSPIDLPKHKTRTTFRTKTHKGDGFNELRFEDKNGDEEIYLHAQRDNNIHINHNKTQYVG
HDDSHTVANNRKHEVCHDEFIHIVNEQTVQVDVNQFEQIGKDRITRIDNSWKEVIHASHFQEIGENKQCDIQNDYTLKAA
GGIHTHTKIHTLQASETVIIKGKAGSIQLDAQGVTITGKILLKGDVSITGGAPEVVTQLEAAVNEGLPLAEDCRAKAMKK
GTA*

                                

Nucleotide sequence: 2172 bp .

                                    >EFF01124 NC_022000:3276277-3278448  (Unknown) [Proteus mirabilis BB2000]
ATGTCTATAACAAATTCAAAAAAATATCAAAGGCTGGGTGATAAGGATGGCAACTCACTGAGTGGGGATAAGACATTAAC
CCATGGCGGTGGCAGAACATACGCGCAAGATCTCGCTTTACAAGAGCAACATAGACAACTCATAGAGAAGATCTTAAGTG
GCGACATGCAAAGTGGCTTAGTGTTTACTTGTCAGATTGGTGGATTACCCGAAGAGACTTTCCAAGTCACGCAATTCGAT
TTACAAGAAGGGATCTCACAACTTTTCACCCTCTCCATCCAAGCCGTCAGTCCACTCTCGGAAATTGATTTTAATGATGT
GTTAGGCATGGCTTCCAGTTTGACGGTAAAGCGAGATGGTCACATTCTGCGTACAGTACAAGGTATCTTAGCGGCGGCAG
AGCAAGGGAATACCGATGGGGTAAAAACCTGGTATCAATTTGTGATACGTCCTGAAATGTGGGTAATGACCCTCAAACAA
GATAGTCGTATTTTTCAAGATACCACCGTTCCTCATATTCTTGAGACATTACTCAATGAGTCTCACATTAAATATGATAA
ACAGCTTTATCACCCAGAAGAGCATTTGCAGCGCGCCTACACCACTCAAAAACGAGAAACAATGTATGACTTTTGGTGCC
GTTTAGCCGCAGAAGAAGGGATTAATTTTTGGTTTGAAGAGGGCCCACAGTTATTTTATAGCGACCGACATTTGGGCATG
AGAGCGGGGATATCCTTAACCTATAACCCACAATCGGATACAGATATCACCGATAGCACCGCCACAACGTGGCGCTATGT
TGAACAACTCTGTAGCGATATTCGCGTTGATAAAGATTACAATCAACTTAGACCTTCCTACCCCTTAAGCCATCAAGTCA
CAGGAGAGGTTCATCAACAACATGAAATATTTGAAAGTTATGGACGCTTTGAAGAAGATGCTCAGGGACAACCCTTTAAT
CAGATACGTTATGAACAATCACAAAATCAGCGTCAAGTAGGCTCTGCGACCACAAACTGCATTGAATTAGCGCCGGGGCG
TATTTTTACCTTGTCTAACCACCCCAGTGCACGCATGAACACCACATGGCAGGTGATTAGTATCTCACATCACGGTGTGC
AACCTCTTGCCGACAACAGTGGCGGTGAAGGAACGCAATTGAGTAATCAGCTTTCCTTTGTGCCGAGTACTCAAGAGTGG
CGCCCGCCTTACCGTTTTAAACCTACCGCCGATGGTGATGAATTAGCCACTGTCGTCGGCCCGCCGGGCGAAGAAATCTA
TACCAATGAGCAAGGTGCCGTTACGGTCTATTTTCATTGGGATAGGCGTGGTAAACCAGACCATAGCGCATCATGCTGGG
TAAGAGTCGCTCAAGGTTGGAATGGGGACGGGTTTGGCTTTATGGCTATTCCTCGTGTTGGGCAAGAAGTGATTATCTCT
TATTTAAACAATGATATAGATAAACCGATTATCACCGGATGTACCTATAATGGTCGCAATCGTTCCCCCATTGATTTACC
TAAACATAAAACCCGTACCACATTTCGCACCAAAACCCATAAAGGTGATGGCTTTAATGAATTGCGCTTTGAGGATAAAA
ATGGCGATGAGGAAATTTATCTTCATGCGCAACGCGATAATAATATTCATATTAATCATAATAAAACCCAATACGTTGGG
CATGATGATAGCCATACTGTCGCCAATAACCGTAAGCATGAAGTGTGTCATGACGAATTTATCCACATCGTCAATGAGCA
AACAGTACAGGTTGATGTCAATCAATTTGAACAAATAGGGAAAGACCGTATCACACGCATTGACAATAGTTGGAAAGAAG
TTATCCACGCTAGTCACTTTCAGGAAATCGGGGAAAACAAGCAGTGTGATATCCAAAATGATTATACCCTGAAAGCCGCA
GGGGGTATCCATACCCATACCAAAATCCACACCTTGCAAGCCAGTGAAACGGTCATTATCAAAGGCAAGGCTGGGTCGAT
TCAATTGGATGCACAAGGTGTGACTATTACAGGTAAAATTCTGCTCAAAGGCGATGTGTCAATAACGGGGGGCGCCCCAG
AAGTGGTTACTCAGTTAGAAGCGGCGGTTAATGAAGGGTTACCATTAGCTGAGGATTGCCGTGCGAAAGCGATGAAAAAA
GGAACGGCCTAA

                                