Detailed information of effector
Summary
| Effector ID | EFF00429  |
| Effector type | - |
| Cognate immunity protein | - |
| Related T6SS (Type) | T6SS17509 (Type i2) |
| Strain | Burkholderia glumae BGR1 |
| Replicon | chromosome |
| Sequence | Protein sequence (162 a.a.); Nucleotide sequence (486 bp) |
| Description | In the presence of E. coli as prey, the relative gene expression level of only tssD1 was increased whereas the relative gene expression levels of the tssD2, tssD4, and tssD5 genes showed little difference regardless of the presence of prey |
| Reference | - |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | BGLU_RS23285 |
| Coordinates (Strand) | 1418865..1419350 (+) |
| NCBI ID | WP_012733890.1 |
| RefSeq | NC_012721 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS_HCP | PF05638.14 | 3.5e-38 | 6..146 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-161 | MAIPAYMWLKDDGGADIKGSVTVQGREGSVELVAFNHGVHIPTDGNTGKLTGTRVHKPITLTKETDASTPYLYKAVTSGQTLKSVEIKWYKIDDAGKEKEYFNTKLDNVKIVAVNPVMYDIKNPDYEKHNHLENVELRYETITWSYKDGNIIHKDTWNERS |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9832 | - | - |
Protein sequence: 162 a.a. .
>EFF00429 NC_012721:1418865-1419350 (Unknown) [Burkholderia glumae BGR1]
MAIPAYMWLKDDGGADIKGSVTVQGREGSVELVAFNHGVHIPTDGNTGKLTGTRVHKPITLTKETDASTPYLYKAVTSGQ
TLKSVEIKWYKIDDAGKEKEYFNTKLDNVKIVAVNPVMYDIKNPDYEKHNHLENVELRYETITWSYKDGNIIHKDTWNER
S*
MAIPAYMWLKDDGGADIKGSVTVQGREGSVELVAFNHGVHIPTDGNTGKLTGTRVHKPITLTKETDASTPYLYKAVTSGQ
TLKSVEIKWYKIDDAGKEKEYFNTKLDNVKIVAVNPVMYDIKNPDYEKHNHLENVELRYETITWSYKDGNIIHKDTWNER
S*
Nucleotide sequence: 486 bp .
>EFF00429 NC_012721:1418865-1419350 (Unknown) [Burkholderia glumae BGR1]
ATGGCAATTCCGGCCTATATGTGGCTCAAGGACGATGGCGGTGCCGACATCAAGGGTTCGGTGACGGTTCAGGGCCGAGA
AGGCAGCGTCGAGCTCGTCGCGTTCAATCACGGCGTGCACATCCCGACCGACGGCAACACGGGCAAGCTCACCGGTACGC
GTGTACACAAGCCGATTACGCTCACGAAGGAAACGGACGCGTCGACGCCATACCTGTACAAGGCCGTGACGAGCGGCCAG
ACGTTGAAGTCGGTCGAAATCAAGTGGTACAAGATCGACGACGCGGGCAAGGAGAAGGAGTACTTCAACACGAAGCTCGA
CAACGTCAAGATCGTGGCCGTCAACCCGGTGATGTACGACATCAAGAACCCGGACTACGAGAAACACAACCACCTCGAAA
ACGTCGAACTGCGCTACGAGACGATCACCTGGTCGTACAAGGACGGCAACATCATCCACAAGGACACCTGGAACGAGCGT
TCCTGA
ATGGCAATTCCGGCCTATATGTGGCTCAAGGACGATGGCGGTGCCGACATCAAGGGTTCGGTGACGGTTCAGGGCCGAGA
AGGCAGCGTCGAGCTCGTCGCGTTCAATCACGGCGTGCACATCCCGACCGACGGCAACACGGGCAAGCTCACCGGTACGC
GTGTACACAAGCCGATTACGCTCACGAAGGAAACGGACGCGTCGACGCCATACCTGTACAAGGCCGTGACGAGCGGCCAG
ACGTTGAAGTCGGTCGAAATCAAGTGGTACAAGATCGACGACGCGGGCAAGGAGAAGGAGTACTTCAACACGAAGCTCGA
CAACGTCAAGATCGTGGCCGTCAACCCGGTGATGTACGACATCAAGAACCCGGACTACGAGAAACACAACCACCTCGAAA
ACGTCGAACTGCGCTACGAGACGATCACCTGGTCGTACAAGGACGGCAACATCATCCACAAGGACACCTGGAACGAGCGT
TCCTGA