SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF00015
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00003 (Type i3)
Strain	Pseudomonas aeruginosa PAO1
Replicon	chromosome
Sequence	Protein sequence (727 a.a.); Nucleotide sequence (2181 bp)
Description	VgrG
Reference	[1] Hood RD, Singh P, Hsu F, et al. A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria. Cell Host Microbe. 2010 Jan 21;7(1):25-37. doi: 10.1016/j.chom.2009.12.007. PMID: 20114026 [2] Hachani A, Lossi NS, Hamilton A, et al. Type VI secretion system in Pseudomonas aeruginosa: secretion and multimerization of VgrG proteins. J Biol Chem. 2011 Apr 8;286(14):12317-27. doi: 10.1074/jbc.M110.193045. Epub 2011 Feb 16. PMID: 21325275

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	PA2685
Coordinates (Strand)	3035752..3037932 (-)
NCBI ID	15597881
RefSeq	NC_002516
Uniprot ID	VGR1C_PSEAE
KEGG ID	pae:PA2685
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	1e-102	66..366
Phage_base_V	PF04717.14	1.7e-14	423..490
Gp5_C	PF06715.14	6.1e-05	566..589

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-726	VAIGQPFATALGCTIIWRAAGALFGPPAAGDYQGRFPMLFSQHTRLVHVDSPLGPEVLQLQRLEGREELGRLFSHELELVSSNPALPLDALLGKPMSLALELPGGSRRYFHGIVARCSQGAGAGQFASYQVTLRPWLWLLTRTSDCRIFQNQKVPDIIKQVFRDLGFSDFEDALSRSYREWEYCVQYRETSFDFVSRLMEQEGIYYWFRHEKKRHILVLSDAYGAHHSPAGYTSVPYYPPSLGHRERDHFFDWHMAREVQPGSLSLNDYDFQRPGTRLEVRSNVGRAHAAADYPLYDYPGEYVQSQDGEHYARTRIEAIQTQYERVRLRGCARGIGAGHLFHLSNYPRLDQNREYLVVGAEYRVVQELYETGNGGGGAQFESELDCIDAGQAFRPLPSTPVPVVRGPQTAVVVGPKGEEIWTDQYGRVKVHFHWDRHDQSNENSSCWMRVSQAWAGKNWGSIQIPRIGQEVIVSFLEGDPDRPIITGRVYNAEQTVPYELPANATQSGTKSRSSKGGTPANFNEIRMEDKKGAEQLFIHAERNQDIEVENDESHWVGHDRTKTIDHDETVHVKHDRTETVDNNETITVHANRSKTVDRNETVRIGMNKTETILMASLQNVGMGRMENVGLGYSLNVGMMMNTVVGLNQSTQVMKKKTLSVGDSYEVSVGGSDDGSKITLDGQSITLGSQRIELTADREILLRCGQSTIRLTPGEIEILSPNVDINC

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.5103	-	-

Protein sequence: 727 a.a. .

                                    >EFF00015 NC_002516:c3037932-3035752  (Unknown) [Pseudomonas aeruginosa PAO1]
VAIGQPFATALGCTIIWRAAGALFGPPAAGDYQGRFPMLFSQHTRLVHVDSPLGPEVLQLQRLEGREELGRLFSHELELV
SSNPALPLDALLGKPMSLALELPGGSRRYFHGIVARCSQGAGAGQFASYQVTLRPWLWLLTRTSDCRIFQNQKVPDIIKQ
VFRDLGFSDFEDALSRSYREWEYCVQYRETSFDFVSRLMEQEGIYYWFRHEKKRHILVLSDAYGAHHSPAGYTSVPYYPP
SLGHRERDHFFDWHMAREVQPGSLSLNDYDFQRPGTRLEVRSNVGRAHAAADYPLYDYPGEYVQSQDGEHYARTRIEAIQ
TQYERVRLRGCARGIGAGHLFHLSNYPRLDQNREYLVVGAEYRVVQELYETGNGGGGAQFESELDCIDAGQAFRPLPSTP
VPVVRGPQTAVVVGPKGEEIWTDQYGRVKVHFHWDRHDQSNENSSCWMRVSQAWAGKNWGSIQIPRIGQEVIVSFLEGDP
DRPIITGRVYNAEQTVPYELPANATQSGTKSRSSKGGTPANFNEIRMEDKKGAEQLFIHAERNQDIEVENDESHWVGHDR
TKTIDHDETVHVKHDRTETVDNNETITVHANRSKTVDRNETVRIGMNKTETILMASLQNVGMGRMENVGLGYSLNVGMMM
NTVVGLNQSTQVMKKKTLSVGDSYEVSVGGSDDGSKITLDGQSITLGSQRIELTADREILLRCGQSTIRLTPGEIEILSP
NVDINC*

                                

Nucleotide sequence: 2181 bp .

                                    >EFF00015 NC_002516:c3037932-3035752  (Unknown) [Pseudomonas aeruginosa PAO1]
GTGGCTATTGGCCAGCCTTTCGCGACGGCACTGGGTTGTACGATCATCTGGCGCGCTGCTGGCGCGTTGTTCGGGCCGCC
CGCCGCAGGTGACTATCAAGGAAGATTCCCCATGCTGTTCTCCCAACACACCCGCCTGGTACACGTGGACTCCCCGCTGG
GACCCGAGGTCCTGCAACTGCAACGGCTGGAGGGTCGCGAAGAGCTGGGCCGGCTGTTCAGCCACGAGCTCGAACTGGTC
TCGAGCAATCCCGCCTTGCCTCTGGATGCCCTGTTGGGCAAGCCGATGAGCCTCGCGCTGGAACTCCCCGGCGGCAGCCG
GCGGTATTTCCACGGCATCGTCGCGCGCTGTAGCCAGGGCGCCGGCGCCGGCCAGTTCGCCAGCTACCAGGTCACCCTGC
GGCCCTGGCTGTGGCTGCTGACGCGCACCTCCGACTGCCGCATCTTCCAGAACCAGAAGGTCCCGGACATCATCAAGCAG
GTGTTCCGCGACCTCGGCTTCTCCGACTTCGAGGACGCCCTGAGCCGCTCCTACCGCGAGTGGGAATACTGTGTGCAATA
CCGCGAGACCAGCTTCGATTTCGTCAGCCGGCTGATGGAGCAGGAAGGCATCTACTACTGGTTCCGCCACGAAAAGAAGC
GCCACATCCTGGTGCTCTCCGATGCCTACGGCGCGCACCACAGCCCGGCCGGCTACACCAGCGTGCCCTATTACCCGCCG
AGCCTCGGCCATCGCGAGCGCGACCACTTCTTCGACTGGCACATGGCGCGCGAGGTCCAGCCCGGCTCGCTGAGCCTCAA
CGACTACGACTTCCAGCGTCCCGGCACCCGCCTGGAAGTGCGCTCCAATGTCGGGCGCGCCCATGCCGCGGCCGACTACC
CGCTCTACGACTACCCTGGCGAGTATGTGCAGAGTCAGGACGGCGAACACTATGCACGGACCCGCATCGAGGCGATCCAG
ACCCAGTACGAGCGAGTTCGCCTGCGGGGATGCGCCAGGGGTATCGGGGCGGGCCATCTGTTCCACCTGAGCAACTACCC
GCGCCTGGACCAGAACCGCGAATACCTGGTGGTTGGCGCCGAGTACCGGGTAGTCCAGGAACTCTACGAGACCGGTAATG
GCGGGGGCGGCGCGCAGTTCGAGAGCGAGCTGGACTGTATCGACGCCGGCCAGGCTTTCCGCCCCTTGCCGTCGACCCCG
GTGCCGGTGGTGCGCGGCCCGCAGACCGCGGTGGTGGTCGGGCCCAAGGGCGAGGAGATCTGGACCGACCAGTATGGCCG
GGTCAAGGTGCATTTCCACTGGGACCGCCACGACCAGTCCAACGAGAACAGCTCCTGCTGGATGCGCGTCTCGCAGGCCT
GGGCCGGGAAGAACTGGGGCTCGATCCAGATCCCGCGGATCGGCCAGGAAGTGATCGTCAGCTTCCTCGAAGGCGACCCG
GACCGGCCGATCATCACCGGGCGGGTCTACAACGCCGAGCAGACGGTGCCCTACGAGCTGCCGGCGAACGCTACCCAGAG
CGGCACCAAGAGCCGTTCGAGCAAGGGCGGCACGCCGGCCAACTTCAACGAGATCCGCATGGAGGACAAGAAGGGCGCCG
AGCAGTTGTTCATCCATGCCGAGAGGAACCAGGACATCGAGGTCGAGAACGACGAGAGCCACTGGGTCGGCCACGACCGG
ACCAAGACCATCGACCACGACGAGACGGTGCATGTGAAGCACGATCGCACCGAGACCGTGGACAACAACGAGACCATCAC
CGTGCATGCCAACCGCAGCAAGACGGTCGACCGCAACGAGACGGTCAGGATCGGCATGAACAAGACGGAAACCATCCTCA
TGGCCTCGTTGCAGAACGTCGGCATGGGGCGCATGGAAAACGTCGGGCTCGGCTACAGCCTGAACGTCGGCATGATGATG
AACACCGTGGTCGGGCTGAACCAGAGCACCCAGGTGATGAAGAAGAAGACCTTGTCGGTGGGCGATAGCTACGAAGTGAG
CGTGGGCGGCAGCGACGATGGCTCTAAGATCACCCTGGACGGTCAGAGCATCACCCTCGGCAGCCAGCGCATCGAGCTGA
CCGCCGACCGCGAGATCCTCTTGCGCTGCGGCCAGAGCACGATCCGCCTGACGCCGGGGGAGATCGAGATCCTCTCGCCC
AATGTCGATATCAACTGTTGA

                                