Detailed information of component protein
Summary
| Component ID | T6CP259664 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS01006  (Type i4b) |
| Strain | Burkholderia pseudomallei K96243 |
| Replicon | chromosome I |
| Sequence | Protein sequence (205 a.a.); Nucleotide sequence (615 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | AQ15_1813 |
| Coordinate (Strand) | 2064432..2065046 (-) |
| NCBI ID | AJX28754.1 |
| GenBank | CP009538 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 3.6e-30 | 55..173 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MNPHVT |
| Transmembrane helix | 7-29 | RYALPLAACILLGGCAAAVPLLG |
| Outside | 30-204 | SAGSAALQMVGVGKPDVPDSQKPPRNLGLTLYAAQNLNAANDNRPLALVVRLYALKDPTSFQQAPFDSFTDPTKEKTALGADLLSVREITLIPGQHYNATEKVSREAQAFGVVALFRDPALQRWKLTFDPAKSEKSGIIIGLHNCAMTVTGGTVIPSQQGMPTQRLDLLSSVNCG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.8508 | pos: 20-21. LGG-CA. | MNPHVTRYALPLAACILLGG |
Protein sequence: 205 a.a.
>T6CP259664 CP009538:c2065046-2064432 [Burkholderia pseudomallei K96243] [TssJ]
MNPHVTRYALPLAACILLGGCAAAVPLLGSAGSAALQMVGVGKPDVPDSQKPPRNLGLTLYAAQNLNAANDNRPLALVVR
LYALKDPTSFQQAPFDSFTDPTKEKTALGADLLSVREITLIPGQHYNATEKVSREAQAFGVVALFRDPALQRWKLTFDPA
KSEKSGIIIGLHNCAMTVTGGTVIPSQQGMPTQRLDLLSSVNCG*
MNPHVTRYALPLAACILLGGCAAAVPLLGSAGSAALQMVGVGKPDVPDSQKPPRNLGLTLYAAQNLNAANDNRPLALVVR
LYALKDPTSFQQAPFDSFTDPTKEKTALGADLLSVREITLIPGQHYNATEKVSREAQAFGVVALFRDPALQRWKLTFDPA
KSEKSGIIIGLHNCAMTVTGGTVIPSQQGMPTQRLDLLSSVNCG*
Nucleotide sequence: 615 bp
>T6CP259664 CP009538:c2065046-2064432 [Burkholderia pseudomallei K96243] [TssJ]
ATGAATCCGCATGTGACTCGTTACGCGCTGCCGCTCGCCGCCTGCATTCTGCTCGGCGGCTGCGCGGCCGCCGTGCCGCT
CCTCGGTTCGGCGGGCAGCGCCGCGCTGCAGATGGTGGGCGTCGGCAAACCCGACGTGCCCGATTCGCAGAAGCCGCCGC
GCAATCTCGGCCTCACGCTCTATGCGGCACAGAACCTCAACGCCGCGAACGACAACCGTCCGCTCGCGCTCGTCGTGCGC
CTCTACGCGCTGAAAGATCCGACGTCGTTCCAGCAGGCGCCGTTCGACAGCTTCACCGATCCGACGAAAGAAAAGACCGC
GCTCGGCGCCGATCTGCTGAGCGTGCGCGAAATCACGCTGATTCCGGGGCAGCACTACAACGCGACCGAAAAGGTGTCGC
GCGAAGCGCAGGCATTCGGCGTCGTCGCGCTTTTTCGCGATCCGGCGCTACAACGCTGGAAACTAACTTTCGATCCGGCG
AAATCCGAGAAATCCGGCATAATCATTGGCCTGCACAACTGCGCGATGACCGTGACGGGCGGCACCGTCATCCCTTCGCA
GCAAGGCATGCCGACGCAGCGGCTCGATTTGCTGTCGTCGGTGAACTGCGGGTGA
ATGAATCCGCATGTGACTCGTTACGCGCTGCCGCTCGCCGCCTGCATTCTGCTCGGCGGCTGCGCGGCCGCCGTGCCGCT
CCTCGGTTCGGCGGGCAGCGCCGCGCTGCAGATGGTGGGCGTCGGCAAACCCGACGTGCCCGATTCGCAGAAGCCGCCGC
GCAATCTCGGCCTCACGCTCTATGCGGCACAGAACCTCAACGCCGCGAACGACAACCGTCCGCTCGCGCTCGTCGTGCGC
CTCTACGCGCTGAAAGATCCGACGTCGTTCCAGCAGGCGCCGTTCGACAGCTTCACCGATCCGACGAAAGAAAAGACCGC
GCTCGGCGCCGATCTGCTGAGCGTGCGCGAAATCACGCTGATTCCGGGGCAGCACTACAACGCGACCGAAAAGGTGTCGC
GCGAAGCGCAGGCATTCGGCGTCGTCGCGCTTTTTCGCGATCCGGCGCTACAACGCTGGAAACTAACTTTCGATCCGGCG
AAATCCGAGAAATCCGGCATAATCATTGGCCTGCACAACTGCGCGATGACCGTGACGGGCGGCACCGTCATCCCTTCGCA
GCAAGGCATGCCGACGCAGCGGCTCGATTTGCTGTCGTCGGTGAACTGCGGGTGA