SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259655
Component protein type	TagF
T6SS ID (Type)	T6SS01006 (Type i4b)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome I
Sequence	Protein sequence (330 a.a.); Nucleotide sequence (990 bp)
Reference	[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	AQ15_1824
Coordinate (Strand)	2077302..2078291 (-)
NCBI ID	AJX27287.1
GenBank	CP009538
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
TagF_N	PF09867.11	1.9e-29	10..139

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-329	MTQTVQAQIAYFGKIPSRGDFVKSAHNPQLLQTLDHWIARAMELLADDPRWKIVYESAKPMHFAFLGSKSRLAIAGHMIASHDASNRRFPFLAATALEVDRPLTFLARSPLAFARLWSRAAAQMQSLLGPQEPPGALQALGDTQVPIDTGGPGSAHDGTFNDFVEHQTLFGLEQMLLASGHPVKLRGAMLALGSLLRPVMQSGTSHIERGLTLPLPTDPFYRSLVAAFWLELVAPFVSRADFELAIFIGTIAERERLIIGFNGASAKTLHSVVDPQTYAEHNIDIDDPEWIDAHAQNDHGISKLVSYLDQPQLSLRVCIDTFREAFAGA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9942	-	-

Protein sequence: 330 a.a.

						>T6CP259655 CP009538:c2078291-2077302 [Burkholderia pseudomallei K96243] [TagF]
MTQTVQAQIAYFGKIPSRGDFVKSAHNPQLLQTLDHWIARAMELLADDPRWKIVYESAKPMHFAFLGSKSRLAIAGHMIA
SHDASNRRFPFLAATALEVDRPLTFLARSPLAFARLWSRAAAQMQSLLGPQEPPGALQALGDTQVPIDTGGPGSAHDGTF
NDFVEHQTLFGLEQMLLASGHPVKLRGAMLALGSLLRPVMQSGTSHIERGLTLPLPTDPFYRSLVAAFWLELVAPFVSRA
DFELAIFIGTIAERERLIIGFNGASAKTLHSVVDPQTYAEHNIDIDDPEWIDAHAQNDHGISKLVSYLDQPQLSLRVCID
TFREAFAGA*					

Nucleotide sequence: 990 bp

						>T6CP259655 CP009538:c2078291-2077302 [Burkholderia pseudomallei K96243] [TagF]
ATGACGCAAACGGTCCAGGCCCAGATCGCCTACTTCGGCAAGATCCCGTCGCGCGGCGACTTCGTGAAGAGCGCGCACAA
CCCGCAGTTGCTGCAAACGCTCGACCACTGGATCGCGCGCGCGATGGAGCTCCTCGCCGACGACCCGCGCTGGAAGATCG
TCTACGAAAGCGCGAAACCGATGCACTTCGCGTTTCTCGGCTCGAAGAGCCGGCTAGCGATCGCGGGCCACATGATCGCG
AGCCACGACGCGTCGAACCGGCGCTTCCCGTTCCTCGCCGCGACGGCGCTCGAAGTCGATCGCCCGCTCACGTTCCTCGC
GCGCAGCCCGCTTGCGTTCGCGCGGCTCTGGTCGCGCGCCGCCGCGCAGATGCAGTCGCTCCTCGGCCCGCAGGAGCCGC
CCGGCGCGCTGCAGGCGCTCGGCGACACGCAAGTGCCGATCGACACGGGCGGCCCGGGCAGCGCGCACGACGGCACGTTC
AACGATTTCGTCGAACACCAGACCCTGTTCGGCCTCGAGCAGATGCTGCTCGCGAGCGGCCATCCGGTGAAGCTGCGCGG
CGCGATGCTCGCGCTCGGCTCGCTGCTGCGCCCGGTGATGCAAAGCGGCACGTCGCACATCGAGCGCGGCCTCACGCTGC
CGCTGCCGACCGATCCGTTCTACCGGAGCCTCGTCGCCGCATTCTGGCTCGAACTCGTCGCGCCGTTCGTGTCGCGCGCC
GATTTCGAGCTCGCGATCTTCATCGGCACGATCGCCGAGCGCGAGCGGCTCATCATCGGCTTCAACGGCGCGTCCGCGAA
AACCCTGCACAGCGTGGTCGATCCGCAGACTTACGCCGAACACAACATCGACATCGACGATCCGGAATGGATCGACGCAC
ATGCGCAAAACGACCATGGGATCAGCAAGCTCGTCAGTTATCTCGATCAACCGCAACTGTCGCTTCGCGTGTGCATCGAC
ACGTTCCGCGAAGCATTCGCCGGAGCGTGA					