Detailed information of component protein
Summary
| Component ID | T6CP259653 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS01262  (Type i1) |
| Strain | Vibrio parahaemolyticus strain 12-297/B |
| Replicon | chromosome |
| Sequence | Protein sequence (264 a.a.); Nucleotide sequence (792 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | B5C30_RS15390 |
| Coordinate (Strand) | 39295..40086 (+) |
| NCBI ID | WP_005495177.1 |
| RefSeq | NZ_MYFG01000475 |
| Uniprot ID | A7N4Q7_VIBCB, A0A0A3EKC1_9VIBR |
| KEGG ID | vca:M892_22465, vha:VIBHAR_05851 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 3.8e-51 | 47..246 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-230 | MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLNIENMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAREIRKGERKLNEMELDRASILVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILERLKADPRKYRHVIEFLYLLLQLGFQGKYGREERGNEKLAEIGNTIYRLVRDERLAEQEKVSLVNLKAKYLKKPLKRVISPK |
| Transmembrane helix | 231-253 | LILGVSAITFAIMYAATYIVIDL |
| Outside | 254-263 | KFQQLLEVYR |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9949 | - | - |
Protein sequence: 264 a.a.
>T6CP259653 NZ_MYFG01000475:39295-40086 [Vibrio parahaemolyticus] [TssL]
MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLNIENMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERKLNEMELDRASILVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILERLKADPRKYRH
VIEFLYLLLQLGFQGKYGREERGNEKLAEIGNTIYRLVRDERLAEQEKVSLVNLKAKYLKKPLKRVISPKLILGVSAITF
AIMYAATYIVIDLKFQQLLEVYR*
MSYAETDVTVVLFQPEPGKPMEVMPAPDLSRNIAVQDLNIENMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERKLNEMELDRASILVIRYCLCAAIDESVCRQEWGANSHWSQNSLLSEFHNETSGGDKFFVILERLKADPRKYRH
VIEFLYLLLQLGFQGKYGREERGNEKLAEIGNTIYRLVRDERLAEQEKVSLVNLKAKYLKKPLKRVISPKLILGVSAITF
AIMYAATYIVIDLKFQQLLEVYR*
Nucleotide sequence: 792 bp
>T6CP259653 NZ_MYFG01000475:39295-40086 [Vibrio parahaemolyticus] [TssL]
ATGAGTTACGCAGAAACCGACGTCACTGTAGTTTTATTCCAACCAGAGCCTGGCAAACCGATGGAGGTGATGCCAGCGCC
TGACTTGTCTCGTAATATCGCGGTACAAGACCTAAACATCGAAAACATGGGCATCAACCCTTTGGTCGACCAGTTTTCAT
GGTTGATCGCCAGCTTGTCTTGTATGTCTTCGATTCCTTGGCTTGATGACCCAATGCCATTTCGTGAACAAGTGGCTCGC
GAGATAAGAAAAGGCGAACGTAAGCTCAACGAAATGGAATTGGATCGCGCATCTATTTTGGTCATCCGATATTGTTTGTG
TGCTGCGATTGATGAATCGGTTTGCCGACAAGAGTGGGGCGCTAACAGCCATTGGAGCCAGAACAGTTTGTTGTCCGAGT
TCCACAATGAAACATCTGGCGGCGATAAGTTCTTCGTTATTCTCGAGCGTTTAAAAGCAGACCCACGCAAATACCGTCAC
GTCATCGAGTTTCTCTATTTGCTGCTTCAACTTGGCTTTCAGGGAAAGTACGGCCGTGAAGAGCGCGGTAACGAAAAGCT
CGCAGAAATTGGTAATACCATTTATCGCTTAGTACGTGATGAACGCTTAGCAGAGCAAGAGAAAGTCTCTCTCGTTAACC
TTAAGGCCAAATACCTTAAGAAGCCGTTAAAAAGGGTGATTTCACCCAAACTGATTTTAGGCGTCAGTGCGATTACCTTC
GCAATCATGTATGCCGCGACTTACATCGTCATAGATTTAAAGTTTCAGCAATTACTTGAAGTGTATCGATAA
ATGAGTTACGCAGAAACCGACGTCACTGTAGTTTTATTCCAACCAGAGCCTGGCAAACCGATGGAGGTGATGCCAGCGCC
TGACTTGTCTCGTAATATCGCGGTACAAGACCTAAACATCGAAAACATGGGCATCAACCCTTTGGTCGACCAGTTTTCAT
GGTTGATCGCCAGCTTGTCTTGTATGTCTTCGATTCCTTGGCTTGATGACCCAATGCCATTTCGTGAACAAGTGGCTCGC
GAGATAAGAAAAGGCGAACGTAAGCTCAACGAAATGGAATTGGATCGCGCATCTATTTTGGTCATCCGATATTGTTTGTG
TGCTGCGATTGATGAATCGGTTTGCCGACAAGAGTGGGGCGCTAACAGCCATTGGAGCCAGAACAGTTTGTTGTCCGAGT
TCCACAATGAAACATCTGGCGGCGATAAGTTCTTCGTTATTCTCGAGCGTTTAAAAGCAGACCCACGCAAATACCGTCAC
GTCATCGAGTTTCTCTATTTGCTGCTTCAACTTGGCTTTCAGGGAAAGTACGGCCGTGAAGAGCGCGGTAACGAAAAGCT
CGCAGAAATTGGTAATACCATTTATCGCTTAGTACGTGATGAACGCTTAGCAGAGCAAGAGAAAGTCTCTCTCGTTAACC
TTAAGGCCAAATACCTTAAGAAGCCGTTAAAAAGGGTGATTTCACCCAAACTGATTTTAGGCGTCAGTGCGATTACCTTC
GCAATCATGTATGCCGCGACTTACATCGTCATAGATTTAAAGTTTCAGCAATTACTTGAAGTGTATCGATAA