SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259651
Component protein type	TssJ
T6SS ID (Type)	T6SS01262 (Type i1)
Strain	Vibrio parahaemolyticus strain 12-297/B
Replicon	chromosome
Sequence	Protein sequence (167 a.a.); Nucleotide sequence (501 bp)
Reference	[1] Jana B, Fridman CM, Bosis E, et al. A modular effector with a DNase domain and a marker for T6SS substrates. Nat Commun. 2019 Aug 9;10(1):3595. doi: 10.1038/s41467-019-11546-6. PMID: 31399579

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	B5C30_RS15380
Coordinate (Strand)	37461..37961 (+)
NCBI ID	WP_011105829.1
RefSeq	NZ_MYFG01000475
Uniprot ID	Q87PT9_VIBPA, A0A227JG17_VIBPH
KEGG ID	vpa:VP1412
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.7e-31	34..150

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-166	MRSIAILLGCALLSGCAMWDQFKESTGITPETSSIELVIEASSVLNVREGGQSSPVILRVHELTSPVLFRSLDFFALFENDKASLGDEYIKRYEYQMQPGEKIHEFLELDPATRAVGFSVAFRDIDGSSWRKVEVIEEKSEYYIKLKLEGSELISDNTRGIEQVYF

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9975	pos: 15-16. LSG-CA.	MRSIAILLGCALLSG

Protein sequence: 167 a.a.

						>T6CP259651 NZ_MYFG01000475:37461-37961 [Vibrio parahaemolyticus] [TssJ]
MRSIAILLGCALLSGCAMWDQFKESTGITPETSSIELVIEASSVLNVREGGQSSPVILRVHELTSPVLFRSLDFFALFEN
DKASLGDEYIKRYEYQMQPGEKIHEFLELDPATRAVGFSVAFRDIDGSSWRKVEVIEEKSEYYIKLKLEGSELISDNTRG
IEQVYF*					

Nucleotide sequence: 501 bp

						>T6CP259651 NZ_MYFG01000475:37461-37961 [Vibrio parahaemolyticus] [TssJ]
ATGCGTAGTATTGCAATATTGCTCGGATGCGCCTTGCTATCCGGTTGTGCCATGTGGGATCAATTTAAAGAATCAACAGG
CATCACGCCTGAAACGTCCTCGATTGAGTTGGTGATTGAGGCGTCTTCGGTTCTAAATGTTCGTGAAGGCGGACAATCCT
CTCCGGTTATTTTGCGAGTTCACGAACTGACGTCACCAGTGTTGTTTCGTAGCTTAGACTTCTTCGCATTGTTTGAGAAC
GATAAAGCATCACTGGGTGATGAATACATCAAACGTTATGAATACCAAATGCAGCCTGGTGAAAAGATCCACGAATTTTT
GGAACTCGACCCAGCAACCCGTGCGGTTGGATTCTCAGTCGCGTTTCGCGATATTGACGGTTCTTCTTGGCGTAAAGTCG
AAGTGATTGAAGAGAAGAGCGAGTACTACATAAAACTCAAGTTAGAAGGCAGTGAGTTAATTTCTGACAACACTCGCGGC
ATTGAACAAGTTTACTTTTAA					