Detailed information of component protein


Summary

Component ID T6CP259650
Component protein type TssA
T6SS ID (Type) T6SS01262 experimental (Type i1)
Strain Vibrio parahaemolyticus strain 12-297/B
Replicon chromosome
Sequence Protein sequence (432 a.a.); Nucleotide sequence (1296 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) B5C30_RS15365
Coordinate (Strand) 33530..34825 (-)
NCBI ID WP_025533653.1
RefSeq NZ_MYFG01000475
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2.3e-24 9..119
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-230 MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGLKGLVEGLNSVLDLCVVYWYSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIKAPSFGRIRRPLNQWIEEIKEEKTKQEKTSQTKPASNPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSR
Transmembrane helix 231-250 FIVYTMIGLLIIAFTSHFIY
Inside 251-431 KQHQYTILKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLTNDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKNSQRKEIERSLLLLNAYLYKINQLQAAEKDNK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9452 - -


  Protein sequence: 432 a.a.    

>T6CP259650 NZ_MYFG01000475:c34825-33530 [Vibrio parahaemolyticus] [TssA]
MFFSDFTRRPIDESNPSGTNPNGLDDFDEIKRQINGLNKVTGRVSWKTVQTLSKEILRTKGKDFRCSCYFTVAAINNDGL
KGLVEGLNSVLDLCVVYWYSAYPEYTKANARISAIEWMVEYTEKRMKKHRVLPEELPLIEAAHQLCLRIEEELRLHYGIK
APSFGRIRRPLNQWIEEIKEEKTKQEKTSQTKPASNPPSAGIRVEIAPPNRPVQKKVEQPKVEEEAKSSRFIVYTMIGLL
IIAFTSHFIYKQHQYTILKERIGSASLTELVTIVNSLSVENKNYQDDLRSVTVERLHTLMSGWTLDPVKVSQVNTIEQLT
NDLTKLYPDSSSAQQLRDNFLGQRSYFEDEFEAIYKRFAYARTVFANIAKQSADKDAKKAYEYSNSLFPLLGRIEYAEKN
SQRKEIERSLLLLNAYLYKINQLQAAEKDNK*

  Nucleotide sequence: 1296 bp    

>T6CP259650 NZ_MYFG01000475:c34825-33530 [Vibrio parahaemolyticus] [TssA]
ATGTTTTTCAGCGACTTCACAAGAAGACCGATCGATGAAAGTAATCCAAGCGGTACCAATCCAAATGGCTTAGACGACTT
TGATGAAATAAAACGTCAAATAAATGGCTTGAACAAAGTAACAGGCCGTGTGTCATGGAAGACAGTTCAGACACTATCAA
AAGAAATCCTTAGAACGAAAGGCAAAGACTTTCGCTGTAGCTGTTACTTTACTGTGGCAGCCATTAATAATGACGGACTA
AAAGGCCTAGTTGAGGGCTTAAATTCTGTGCTCGACTTGTGTGTTGTTTATTGGTACTCCGCCTATCCTGAGTACACAAA
AGCTAATGCCCGTATTAGTGCGATTGAATGGATGGTTGAGTACACTGAAAAACGAATGAAGAAACACCGAGTCCTGCCGG
AAGAACTCCCCCTAATTGAAGCGGCTCATCAGCTTTGTCTACGTATCGAAGAGGAACTTCGCCTTCACTATGGTATTAAG
GCACCTTCTTTTGGACGTATTCGTCGTCCCCTAAATCAGTGGATTGAAGAGATTAAAGAAGAAAAGACGAAACAAGAGAA
AACCTCACAAACAAAACCTGCGTCAAATCCTCCTTCAGCAGGCATCCGAGTCGAAATAGCACCGCCCAATAGACCTGTTC
AAAAGAAAGTAGAGCAGCCTAAAGTGGAAGAAGAGGCCAAATCAAGCCGATTCATTGTCTATACAATGATCGGATTGCTG
ATCATAGCTTTTACGTCTCACTTCATATACAAACAGCATCAATACACAATACTCAAGGAAAGAATTGGGTCAGCATCACT
TACTGAATTAGTGACGATTGTTAACTCGCTCAGTGTCGAAAACAAAAATTACCAAGATGACTTGCGCTCTGTCACGGTTG
AGCGTCTTCATACTTTGATGTCAGGTTGGACATTAGATCCAGTCAAAGTGAGCCAGGTCAATACCATTGAACAGTTAACG
AATGACCTGACCAAGCTCTATCCAGACTCATCCTCTGCACAACAACTTCGCGATAATTTTTTAGGCCAACGTTCTTATTT
TGAAGATGAATTTGAGGCTATATACAAACGTTTTGCATATGCTAGAACGGTCTTTGCCAACATTGCAAAACAAAGTGCAG
ACAAGGACGCTAAGAAAGCATATGAGTACAGCAATTCGCTATTTCCCCTTCTTGGGCGAATTGAATATGCAGAAAAGAAC
TCTCAGCGAAAGGAAATTGAACGATCACTTCTTTTGCTGAACGCTTACCTATATAAAATCAACCAGCTACAAGCTGCAGA
AAAAGACAATAAATAA