Detailed information of component protein


Summary

Component ID T6CP259643
Component protein type TssA
T6SS ID (Type) T6SS01262 experimental (Type i1)
Strain Vibrio parahaemolyticus strain 12-297/B
Replicon chromosome
Sequence Protein sequence (525 a.a.); Nucleotide sequence (1575 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) B5C30_RS15325
Coordinate (Strand) 22753..24327 (+)
NCBI ID WP_085577067.1
RefSeq NZ_MYFG01000475
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasJ PF16989.7 9.1e-73 247..497
ImpA_N PF06812.14 1.3e-26 10..120
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-524 MSVIEIERLLAPISDVNPVGEDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEEFGLQGFVQGLKLLSEALNRFGVELYPSRKRGRDGAVEWLNHQFKLVSSRFAESAQSWDLVSGCISTIEEVQRQYDDIYQDSEADFFEIRSQLNALSQQATVDEQSDGAMATIEQTAPLTAAQPVAVETPAQSEVQTVVEKAVPSAPKPAPVKKTVTKEVDVDTDFSSPTASKRTLKKVAEVMIHSNPSEPLAYRIYRHLTWDDIDGLPDHQSNETPLSLAVSSDQQAEYRDKASQESDIDTVKRLERTLTDAPFWLTGHYFVYSMLNNLGFNDAALAVKQEVKRFVDSLEGIELLTFKNSIPFADEATLSWLSTQAATSSSAQSVVQTIVITDEDSLPMEDITLANLGEYAAELAHKLELDFSGRGQFMLHLQLIAAYQSVGLYPLCLPYLEKAWEVQQAFNLASWEPHLSSQLEDLIRKTLHQLFRNKDLLPEQYEKWKAIYD


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9961 - -


  Protein sequence: 525 a.a.    

>T6CP259643 NZ_MYFG01000475:22753-24327 [Vibrio parahaemolyticus] [TssA]
MSVIEIERLLAPISDVNPVGEDARYEFCYEMMESEVKKFGSLFGETVDWSVVKTHATEVLEHHSKDLKAICYLVRALTEE
FGLQGFVQGLKLLSEALNRFGVELYPSRKRGRDGAVEWLNHQFKLVSSRFAESAQSWDLVSGCISTIEEVQRQYDDIYQD
SEADFFEIRSQLNALSQQATVDEQSDGAMATIEQTAPLTAAQPVAVETPAQSEVQTVVEKAVPSAPKPAPVKKTVTKEVD
VDTDFSSPTASKRTLKKVAEVMIHSNPSEPLAYRIYRHLTWDDIDGLPDHQSNETPLSLAVSSDQQAEYRDKASQESDID
TVKRLERTLTDAPFWLTGHYFVYSMLNNLGFNDAALAVKQEVKRFVDSLEGIELLTFKNSIPFADEATLSWLSTQAATSS
SAQSVVQTIVITDEDSLPMEDITLANLGEYAAELAHKLELDFSGRGQFMLHLQLIAAYQSVGLYPLCLPYLEKAWEVQQA
FNLASWEPHLSSQLEDLIRKTLHQLFRNKDLLPEQYEKWKAIYD*

  Nucleotide sequence: 1575 bp    

>T6CP259643 NZ_MYFG01000475:22753-24327 [Vibrio parahaemolyticus] [TssA]
ATGTCTGTGATCGAAATTGAACGTTTACTTGCGCCTATCTCTGACGTAAATCCTGTCGGTGAAGATGCTCGTTACGAGTT
TTGTTATGAAATGATGGAATCCGAAGTGAAGAAGTTCGGATCCTTGTTTGGCGAAACGGTAGATTGGAGTGTCGTAAAAA
CGCACGCCACTGAAGTTCTTGAACACCACAGCAAAGATTTAAAAGCAATTTGTTACCTTGTTCGCGCGCTTACAGAAGAG
TTTGGTCTGCAAGGTTTCGTGCAAGGTCTAAAGCTGCTTAGTGAAGCGTTAAATCGTTTTGGCGTTGAGCTGTACCCGTC
GCGAAAACGGGGTAGAGATGGTGCGGTAGAGTGGTTGAACCATCAGTTTAAATTGGTGAGCAGCAGGTTTGCAGAAAGCG
CTCAGTCATGGGATTTGGTTTCAGGTTGTATTTCGACTATCGAAGAAGTCCAACGTCAGTACGACGATATTTACCAAGAC
TCCGAAGCGGACTTCTTTGAAATTCGTAGTCAACTCAATGCGCTATCTCAACAAGCGACTGTCGATGAACAAAGCGATGG
TGCAATGGCTACCATCGAACAAACAGCTCCGCTAACGGCTGCACAGCCTGTAGCAGTAGAAACTCCGGCACAAAGTGAAG
TTCAAACCGTTGTAGAAAAAGCGGTTCCGTCGGCTCCTAAACCTGCACCAGTTAAGAAAACAGTAACAAAAGAAGTCGAT
GTCGATACGGATTTTTCATCGCCGACCGCTTCTAAACGTACACTTAAGAAAGTTGCGGAAGTTATGATTCATTCGAATCC
AAGTGAGCCGCTAGCTTACCGAATTTATCGTCACCTAACTTGGGATGATATTGATGGTCTTCCTGATCATCAAAGCAATG
AAACGCCATTGAGTTTGGCTGTGTCGTCTGATCAGCAGGCTGAGTACCGCGATAAAGCCTCTCAAGAAAGCGACATTGAT
ACAGTTAAGCGTTTGGAGCGCACGTTAACCGATGCTCCGTTTTGGCTGACGGGTCACTACTTTGTTTATTCGATGTTAAA
CAACCTAGGCTTTAATGACGCAGCGCTCGCTGTGAAGCAAGAAGTGAAGCGTTTTGTTGATTCATTAGAGGGCATTGAAC
TCCTCACATTTAAGAATTCTATACCATTTGCAGATGAAGCAACGTTGAGCTGGTTATCGACTCAAGCTGCAACTTCTTCA
AGTGCTCAATCTGTCGTTCAAACGATAGTCATCACAGATGAAGACTCACTGCCAATGGAAGATATCACCCTAGCCAACTT
AGGGGAGTATGCCGCAGAACTCGCACACAAACTCGAACTGGATTTCTCGGGACGTGGGCAGTTTATGCTGCATTTACAAT
TGATAGCAGCTTACCAGTCTGTTGGATTATACCCGTTGTGTTTGCCTTACCTTGAAAAAGCTTGGGAAGTGCAACAAGCG
TTTAATCTCGCGAGCTGGGAACCCCACTTATCGTCACAGTTAGAAGACCTGATTCGAAAAACACTGCATCAGTTATTCAG
AAACAAGGATCTTTTGCCTGAACAGTACGAAAAGTGGAAAGCAATTTATGACTAA