SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259642
Component protein type	TssC
T6SS ID (Type)	T6SS17510 (Type Unknown)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (727 a.a.); Nucleotide sequence (2181 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866 [2] Kim N, Han G, Jung H, et al. T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1. Plants (Basel). 2021 Dec 23;11(1). pii: 34. doi: 10.3390/plants11010034. PMID: 35009038

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS21550
Coordinate (Strand)	898308..900488 (-)
NCBI ID	WP_012733561.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
VipB	PF05943.14	4.9e-128	279..604
VipB_2	PF18945.2	1e-44	614..724
T6SS_VipA	PF05591.14	3.3e-30	11..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-726	MSESVQKKLLRIRPPRVRITYDVETGGAIEKTELPFIVGILADLKGEREDAEHYPSLKERSMIEIDRDNFDDVMKKIQPRATFTQLKQDDAALKALLVQDGDNALIFPNMEAFEPLKVIKALPSLNDIYVNRGKIRDLQARAETSDPVSKVLDAMIQGAVLGQAAVAAQPAVPDQPEVPAVPAVPADGDKPAVEAVPGKPAVKGTPAVAAKDAVPALQDSAVETALAALADLRKKDVAVPDDLNVTKFAEQFKLFYVASNDDVAKITEARKRGAIAVIDALIAEIDKQLSDAVSSIMHCDSFKTMEATWRGLNYLVMNTETSTTLKLRVFDAQKEELRSDMVKAVEFDQSALFKMIYEAEYGTYGGKPYSLLVGDYAIAKTPQDIEFVTKMAEVAAAAHAPFLAQASTDLFSLANFGELDKPRDLKKIFEGEEYEAWREFRDLEDARYVTLALPRALLRLPYGMPTKRSTTPCDGINFDERIGASGKDDTLYGKDASEPLSYAKPAPDHLLWGNPAYLLAQRITNAFAHYSWTAAIRGEEGGGLVENLPLYTYTSDNGSTELLCPTEISITDRREKELNDLGFISLCHCKGTGKAAFFGGQTTNQPKKYFSDAANANARISALLPYMLAASRFAHYIKVIMRQKIGSFQTRGNVEDYLNSWISNYVLLDDNASQDAKAAYPLREAKVQVSEVPGEPGSYRAVVFLKPHFQLEELTTSIRLVANLPK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9981	-	-

Protein sequence: 727 a.a.

						>T6CP259642 NC_012721:c900488-898308 [Burkholderia glumae BGR1] [TssC]
MSESVQKKLLRIRPPRVRITYDVETGGAIEKTELPFIVGILADLKGEREDAEHYPSLKERSMIEIDRDNFDDVMKKIQPR
ATFTQLKQDDAALKALLVQDGDNALIFPNMEAFEPLKVIKALPSLNDIYVNRGKIRDLQARAETSDPVSKVLDAMIQGAV
LGQAAVAAQPAVPDQPEVPAVPAVPADGDKPAVEAVPGKPAVKGTPAVAAKDAVPALQDSAVETALAALADLRKKDVAVP
DDLNVTKFAEQFKLFYVASNDDVAKITEARKRGAIAVIDALIAEIDKQLSDAVSSIMHCDSFKTMEATWRGLNYLVMNTE
TSTTLKLRVFDAQKEELRSDMVKAVEFDQSALFKMIYEAEYGTYGGKPYSLLVGDYAIAKTPQDIEFVTKMAEVAAAAHA
PFLAQASTDLFSLANFGELDKPRDLKKIFEGEEYEAWREFRDLEDARYVTLALPRALLRLPYGMPTKRSTTPCDGINFDE
RIGASGKDDTLYGKDASEPLSYAKPAPDHLLWGNPAYLLAQRITNAFAHYSWTAAIRGEEGGGLVENLPLYTYTSDNGST
ELLCPTEISITDRREKELNDLGFISLCHCKGTGKAAFFGGQTTNQPKKYFSDAANANARISALLPYMLAASRFAHYIKVI
MRQKIGSFQTRGNVEDYLNSWISNYVLLDDNASQDAKAAYPLREAKVQVSEVPGEPGSYRAVVFLKPHFQLEELTTSIRL
VANLPK*					

Nucleotide sequence: 2181 bp

						>T6CP259642 NC_012721:c900488-898308 [Burkholderia glumae BGR1] [TssC]
ATGAGTGAAAGCGTGCAGAAGAAGCTCCTGCGTATTCGCCCTCCCCGGGTGAGGATCACGTACGACGTGGAGACGGGTGG
CGCCATCGAGAAGACGGAGCTGCCGTTCATCGTCGGCATCCTGGCCGACCTGAAGGGCGAGCGCGAAGATGCGGAACACT
ATCCGTCGCTGAAGGAGCGGTCGATGATCGAGATCGATCGCGACAACTTCGACGACGTGATGAAGAAGATCCAGCCGCGC
GCCACCTTCACGCAGCTGAAGCAGGACGACGCGGCGCTGAAGGCTCTGCTCGTGCAGGACGGCGACAACGCGCTGATCTT
CCCGAACATGGAAGCGTTCGAACCGCTGAAGGTGATCAAGGCGCTGCCGTCGCTGAACGACATCTACGTCAACCGCGGCA
AGATCCGCGACCTGCAGGCGCGCGCGGAAACGAGCGACCCCGTCAGCAAGGTGCTCGATGCGATGATCCAAGGCGCGGTG
CTCGGCCAGGCCGCCGTGGCGGCCCAGCCCGCGGTGCCCGATCAGCCCGAGGTGCCAGCCGTGCCGGCCGTGCCGGCCGA
CGGCGACAAGCCGGCCGTCGAGGCGGTGCCGGGCAAGCCCGCCGTCAAGGGCACGCCGGCGGTGGCGGCCAAGGATGCCG
TGCCGGCCCTGCAGGACTCGGCCGTCGAGACCGCGCTCGCGGCGCTCGCCGATCTGCGCAAGAAGGACGTCGCGGTGCCC
GACGATCTGAACGTCACGAAGTTCGCGGAGCAGTTCAAGCTCTTCTACGTCGCCAGCAACGACGACGTGGCGAAGATCAC
CGAGGCGCGCAAGCGCGGCGCGATTGCCGTGATCGACGCGCTGATCGCCGAGATCGACAAGCAGCTCAGCGACGCGGTGT
CGAGCATCATGCACTGCGACAGCTTCAAGACGATGGAGGCGACCTGGCGCGGCCTCAACTACCTGGTGATGAACACCGAG
ACCAGCACGACGCTGAAGCTGCGCGTGTTCGACGCCCAGAAGGAAGAGCTGCGCAGCGACATGGTGAAGGCGGTCGAGTT
CGACCAGAGCGCCCTCTTCAAGATGATCTACGAGGCCGAGTACGGCACCTACGGCGGCAAGCCGTACAGCCTGCTGGTGG
GCGACTACGCGATCGCCAAGACCCCGCAGGACATCGAGTTCGTCACCAAGATGGCCGAGGTGGCCGCCGCCGCCCACGCG
CCGTTCCTCGCGCAGGCATCGACCGATCTGTTCAGCCTCGCGAACTTCGGCGAGCTCGACAAGCCGCGCGATCTGAAGAA
GATCTTCGAGGGCGAGGAGTACGAGGCATGGCGCGAATTCCGCGACCTCGAGGACGCGCGCTACGTCACGCTCGCGCTGC
CGCGCGCGCTGCTGCGCCTGCCCTACGGCATGCCCACCAAGCGCAGCACCACGCCGTGCGACGGCATCAACTTCGACGAG
CGCATCGGCGCCTCGGGCAAGGACGACACGCTCTACGGCAAGGACGCCAGCGAGCCGCTCAGCTACGCGAAGCCGGCCCC
GGACCATCTGCTGTGGGGCAACCCGGCCTACCTGCTCGCGCAGCGCATCACCAACGCGTTCGCGCACTACAGCTGGACGG
CGGCGATCCGCGGCGAGGAAGGCGGCGGCCTGGTGGAGAACCTGCCGCTCTATACCTACACCTCCGACAACGGCAGCACC
GAGCTGCTCTGCCCGACCGAGATCTCGATCACCGATCGCCGCGAGAAGGAGCTCAACGACCTCGGCTTCATCTCGCTCTG
CCATTGCAAGGGCACCGGCAAGGCGGCGTTCTTCGGCGGCCAGACCACCAACCAGCCGAAGAAGTACTTCTCGGACGCGG
CCAACGCCAACGCCCGGATCTCCGCGCTGCTGCCGTACATGCTCGCCGCGTCGCGCTTCGCGCATTACATCAAGGTCATC
ATGCGTCAGAAGATCGGCTCGTTCCAGACCCGCGGCAACGTCGAGGACTACCTCAACTCGTGGATCTCGAACTACGTGCT
GCTCGACGACAACGCCTCGCAGGACGCCAAGGCCGCCTACCCGCTGCGTGAAGCGAAGGTGCAGGTGAGCGAGGTGCCGG
GCGAGCCGGGCTCGTACCGCGCCGTGGTGTTCCTCAAGCCGCACTTCCAGCTCGAGGAGCTGACGACTTCGATCCGGCTC
GTGGCCAACCTGCCGAAATAA					