SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259639
Component protein type	TssF
T6SS ID (Type)	T6SS17510 (Type Unknown)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (617 a.a.); Nucleotide sequence (1851 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866 [2] Kim N, Han G, Jung H, et al. T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1. Plants (Basel). 2021 Dec 23;11(1). pii: 34. doi: 10.3390/plants11010034. PMID: 35009038

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS21535
Coordinate (Strand)	895419..897269 (-)
NCBI ID	WP_012733558.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.9e-168	7..612

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-616	MQAATVTLSDCFREELASLRTESVQFSEKHRELARTLGLNAREASDPQVELLLQSFAFLAARLRHQVELDKARLPNTLLSFLYPHLEAPIPSMLIARIDVKPDGTDYAKEQLLQRGRTVNALTANQLGQNVECRFQTCYDTPLLPLRIDAVVLESASEYAFAGAGSSSRSVLRVRLSAAGVGTLQSKGRGPRRLRFYIDDAQPDAVALYEMIALHLESIHVLPAAPLTPGAQPLRQLPAEALRWLGMEPDEAVLSANPHTHPGYRLLQEYFAFPEKFGFFEIGRLDELDFSGVSEYFDLLLMLDMPFDPKHHFSAHSLVLNCVPLVNLFTQRIEPIALDHSEYEYRVTGDFTNHRYCEIHSIRELESISSEGKPRPIAPYFAMDNFASLEGQDYFYLCRRLPATAANVAGSEMFVSFLDQQFNLGQLTDEVVGGTALCTNRRLPERLMSGSALHLEGGGPVNRIVAMTKPTQHHTPPQIGSRPWSLVSQLALNHLSLDGGALALAALKDILRLHVGPHRESGWKQIDAITELRSRSIMRHVGRDGWRGFVRGSELTVVMEPIDIGAGSVVRFCSVLREFLRGYASVNHLVEVALETRNLKGSPKQWQASAGTAIAL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9949	-	-

Protein sequence: 617 a.a.

						>T6CP259639 NC_012721:c897269-895419 [Burkholderia glumae BGR1] [TssF]
MQAATVTLSDCFREELASLRTESVQFSEKHRELARTLGLNAREASDPQVELLLQSFAFLAARLRHQVELDKARLPNTLLS
FLYPHLEAPIPSMLIARIDVKPDGTDYAKEQLLQRGRTVNALTANQLGQNVECRFQTCYDTPLLPLRIDAVVLESASEYA
FAGAGSSSRSVLRVRLSAAGVGTLQSKGRGPRRLRFYIDDAQPDAVALYEMIALHLESIHVLPAAPLTPGAQPLRQLPAE
ALRWLGMEPDEAVLSANPHTHPGYRLLQEYFAFPEKFGFFEIGRLDELDFSGVSEYFDLLLMLDMPFDPKHHFSAHSLVL
NCVPLVNLFTQRIEPIALDHSEYEYRVTGDFTNHRYCEIHSIRELESISSEGKPRPIAPYFAMDNFASLEGQDYFYLCRR
LPATAANVAGSEMFVSFLDQQFNLGQLTDEVVGGTALCTNRRLPERLMSGSALHLEGGGPVNRIVAMTKPTQHHTPPQIG
SRPWSLVSQLALNHLSLDGGALALAALKDILRLHVGPHRESGWKQIDAITELRSRSIMRHVGRDGWRGFVRGSELTVVME
PIDIGAGSVVRFCSVLREFLRGYASVNHLVEVALETRNLKGSPKQWQASAGTAIAL*					

Nucleotide sequence: 1851 bp

						>T6CP259639 NC_012721:c897269-895419 [Burkholderia glumae BGR1] [TssF]
ATGCAAGCCGCCACCGTCACGCTCAGCGACTGCTTCCGCGAGGAACTCGCCTCGCTGCGCACCGAGTCGGTCCAGTTCAG
CGAGAAGCATCGCGAGCTGGCCCGCACGCTCGGCCTCAACGCGCGCGAGGCGAGCGACCCGCAGGTCGAGCTGCTGCTGC
AGTCGTTCGCCTTTCTCGCGGCACGCCTGCGGCACCAGGTGGAACTGGACAAGGCGCGCCTGCCCAATACCCTGCTGTCG
TTCCTCTATCCGCACCTGGAGGCGCCGATCCCGTCGATGCTGATCGCGCGCATCGACGTGAAGCCCGACGGCACCGACTA
TGCCAAGGAACAACTGCTGCAGCGCGGCCGCACCGTGAATGCGCTGACCGCCAACCAGCTCGGCCAGAACGTCGAGTGCC
GGTTCCAGACCTGCTACGACACCCCGCTGCTGCCGCTGCGCATCGACGCCGTGGTGCTCGAATCGGCGAGCGAATACGCG
TTCGCGGGCGCCGGCTCGTCGTCGCGCTCGGTGCTGCGGGTCCGTCTCTCGGCGGCCGGCGTCGGCACCCTGCAGTCGAA
GGGGCGCGGGCCGCGCCGGCTGCGCTTCTACATCGACGACGCGCAGCCCGACGCGGTGGCGCTCTACGAGATGATCGCGC
TGCACCTGGAGAGCATCCACGTGCTGCCGGCCGCGCCGCTCACGCCCGGCGCGCAGCCCCTGCGGCAGCTGCCGGCCGAG
GCGCTGCGCTGGCTCGGCATGGAACCCGACGAGGCGGTGCTCAGCGCCAACCCGCACACGCACCCCGGCTATCGCCTGCT
GCAGGAGTATTTCGCGTTTCCGGAGAAGTTCGGCTTCTTCGAGATCGGCCGGCTCGACGAGCTCGACTTCAGCGGCGTGA
GCGAGTATTTCGACCTGCTGCTGATGCTCGACATGCCGTTCGACCCCAAGCATCATTTCTCGGCCCACTCGCTGGTGCTG
AACTGCGTGCCGCTCGTCAACCTCTTCACGCAGCGCATCGAGCCGATCGCGCTCGATCACAGCGAGTACGAATACCGCGT
GACGGGCGACTTCACGAATCACCGCTACTGCGAGATCCATTCGATCCGCGAGCTCGAATCGATCTCGAGCGAGGGCAAGC
CGCGTCCGATCGCGCCTTACTTCGCGATGGACAACTTCGCCAGCCTGGAAGGCCAGGACTATTTCTATCTGTGCCGGCGC
CTGCCGGCCACCGCCGCCAACGTCGCGGGCAGCGAGATGTTCGTCTCGTTCCTCGACCAGCAGTTCAACCTCGGCCAGCT
CACCGACGAGGTGGTGGGCGGCACCGCGCTCTGCACCAACCGGCGGCTGCCGGAGCGCCTGATGTCGGGCAGCGCGCTGC
ACCTGGAAGGCGGCGGCCCGGTGAACCGGATCGTCGCGATGACCAAGCCGACCCAGCACCACACGCCGCCGCAGATCGGC
AGCCGCCCGTGGTCGCTGGTCTCGCAGCTCGCGCTCAACCACCTCTCGCTCGACGGCGGCGCGCTCGCGCTCGCCGCGCT
GAAGGACATCCTGCGGCTGCACGTCGGCCCGCATCGCGAGAGCGGCTGGAAGCAGATCGACGCGATCACCGAGCTGCGCA
GCCGCAGCATCATGCGCCACGTCGGGCGCGACGGCTGGCGCGGCTTCGTGCGCGGCTCGGAGCTGACGGTGGTGATGGAG
CCGATCGACATCGGCGCGGGCAGCGTGGTGCGGTTCTGCTCGGTGCTGCGCGAATTCCTGCGCGGCTACGCGAGCGTCAA
TCATCTGGTCGAAGTCGCGCTCGAAACGCGTAACCTGAAGGGGAGCCCCAAGCAATGGCAAGCATCGGCCGGCACCGCTA
TCGCACTGTAA					