SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259638
Component protein type	TssG
T6SS ID (Type)	T6SS17510 (Type Unknown)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (380 a.a.); Nucleotide sequence (1140 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866 [2] Kim N, Han G, Jung H, et al. T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1. Plants (Basel). 2021 Dec 23;11(1). pii: 34. doi: 10.3390/plants11010034. PMID: 35009038

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS21525
Coordinate (Strand)	894260..895399 (-)
NCBI ID	WP_080569382.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	5.8e-51	39..353

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-379	IRGLQRVPVDAPHAVRYRQAPADRAAPAVSRRPVGRVPRQRVHARGGAAARAVRAAPGLRGAPRPAARHCRRGRRPLARRRGRARRAARFEITTANLCVASVSGPMPEPFTEWARELAALRATAMADFFDIFNQRANVLRYELKQRLTPGLDDTPPERTEAAGCLAALVGLGSPQLSSRVPLPQRAWLGIAGLLANRRRQGATLVEVLRVALGARVELTENVGAWHDLDERDRCALGRRNHRLGQTSVVGRRVWDQQARVRLEIGVLDYAALCRLLPPAPNPAPAAVPGLAAPAAPGYAMLSGLVKLLVDRLADVEIVLQVDDASIPDAGTPLPQPARGTDGSSMRLGQSAWLAGAPAHRHATRPVRFLITTRDSGSLA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6058	-	-

Protein sequence: 380 a.a.

						>T6CP259638 NC_012721:c895399-894260 [Burkholderia glumae BGR1] [TssG]
IRGLQRVPVDAPHAVRYRQAPADRAAPAVSRRPVGRVPRQRVHARGGAAARAVRAAPGLRGAPRPAARHCRRGRRPLARR
RGRARRAARFEITTANLCVASVSGPMPEPFTEWARELAALRATAMADFFDIFNQRANVLRYELKQRLTPGLDDTPPERTE
AAGCLAALVGLGSPQLSSRVPLPQRAWLGIAGLLANRRRQGATLVEVLRVALGARVELTENVGAWHDLDERDRCALGRRN
HRLGQTSVVGRRVWDQQARVRLEIGVLDYAALCRLLPPAPNPAPAAVPGLAAPAAPGYAMLSGLVKLLVDRLADVEIVLQ
VDDASIPDAGTPLPQPARGTDGSSMRLGQSAWLAGAPAHRHATRPVRFLITTRDSGSLA*					

Nucleotide sequence: 1140 bp

						>T6CP259638 NC_012721:c895399-894260 [Burkholderia glumae BGR1] [TssG]
ATACGAGGACTTCAACGTGTTCCAGTTGATGCGCCTCATGCTGTCCGATACCGACAAGCGCCTGCCGATCGGGCGGCGCC
TGCGGTTTCGCGCCGACCTGTCGGCCGCGTTCCCCGCCAGCGAGTTCACGCGCGTGGCGGCGCCGCCGCCCGTGCCGTCC
GTGCCGCCCCCGGCCTCCGGGGCGCACCTCGACCCGCCGCCCGGCACTGCCGCCGCGGGCGGCGCCCCCTTGCCCGCCGC
CGAGGCCGTGCCCGCCGCGCCGCGCGCTTCGAGATCACCACCGCGAACCTGTGCGTGGCCAGCGTGAGCGGGCCGATGCC
CGAGCCGTTCACCGAGTGGGCGCGCGAACTCGCGGCGCTGCGCGCGACCGCGATGGCCGACTTCTTCGACATCTTCAACC
AGCGCGCCAACGTGCTGCGCTACGAGCTGAAGCAGCGCCTCACGCCGGGCCTCGACGACACGCCGCCGGAGCGCACCGAG
GCGGCCGGCTGCCTGGCGGCGCTGGTCGGCCTGGGCAGCCCGCAGCTGAGCAGCCGGGTGCCGCTGCCGCAGCGCGCCTG
GCTCGGCATCGCGGGCCTGCTGGCGAACCGGCGCCGGCAGGGCGCGACGCTGGTGGAGGTGCTGCGCGTGGCGCTCGGCG
CGCGCGTCGAGCTGACCGAGAACGTCGGCGCGTGGCACGACCTCGACGAGCGCGACCGCTGCGCGCTCGGCCGCCGCAAT
CACCGGCTCGGCCAGACCAGCGTGGTGGGGCGCCGCGTGTGGGACCAGCAGGCGCGCGTGCGCCTCGAGATCGGCGTGCT
CGACTACGCGGCGCTGTGCCGCCTGCTGCCGCCCGCGCCGAACCCGGCGCCGGCCGCCGTGCCCGGCCTGGCCGCGCCGG
CCGCGCCCGGTTACGCGATGCTCAGCGGCCTCGTGAAGCTGCTGGTCGATCGCCTCGCCGACGTCGAGATCGTGCTGCAG
GTGGACGACGCGAGCATCCCCGACGCCGGCACGCCGCTGCCCCAGCCCGCGCGCGGCACCGACGGCAGCTCGATGCGGCT
CGGCCAGAGCGCCTGGCTCGCCGGCGCGCCCGCGCATCGCCACGCCACGCGGCCGGTGCGCTTTCTCATCACCACCCGCG
ATTCCGGGAGCCTCGCATGA					