SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259634
Component protein type	TssM
T6SS ID (Type)	T6SS17510 (Type Unknown)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (1340 a.a.); Nucleotide sequence (4020 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866 [2] Kim N, Han G, Jung H, et al. T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1. Plants (Basel). 2021 Dec 23;11(1). pii: 34. doi: 10.3390/plants11010034. PMID: 35009038

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS21480
Coordinate (Strand)	880140..884159 (-)
NCBI ID	WP_012733548.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	2.2e-51	172..413

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-14	VDAILKSTAWLKAQ
Transmembrane helix	15-37	TGWAPAAGTLKLVLGVLAALLAL
Inside	38-409	AVLAKLAGKLWARLGPLLKAPAVPPLCGCEPAAETRAKGWIERASLALDYLRTGREWRYATPWLLVLGQAGAGKTSLLESVGPQHTQPLGERQQSLALEGASWHAIDQSLVLDPLGKWPEAAASAMLDSGTADGADARRWLKFLDQLDALRPERALDGIVLVVCATTFLQRDPARLMAAAQNARQQLRTIEDRLEFALPVYVVVTACDHVEGFGAFWRSQAPQRHTEMVGWSAPSQTFDTPPTQWGAQIFAAIGAQLRALQVETAAHCERIAADDADPMFLYPLRFAQLQGPFEQWLSVVFQVSAWETTFFLRGVYFTGLVAEPGEPLEARDGPRRDVAFVRDLMLEKVLAEKHLARPTRAGVWSRNLLIRR
Transmembrane helix	410-432	VQWACVIGFSLLLLAFAVRVWMI
Outside	433-1339	DAQIGRVIQALERMQQLQAPAPGAGCIAQAPVYQLIEQVAQIDADASSWLLPASLFDSRLSHQSARRVIDTALRKVILPGLACQLSQRAYALGNEANRGISAGLGYTQALDQLQGFVQQADQYERNAARFQRLLGKTPYARERVPLPGFFDLVEYAYRTPVPPYLRAHAGLLPVTLGDLTERDFSGEIATPPQLRQSVGNHIVALAAQAGSLLQAELQSGLPLLEQLQARQQPILAHVRQFTHWLNWIRTAWLGSSAAANPILTVQNQLLASLQPLVADFGYPSYIQAQVSAQFDAAHQYPLAMQTLNALTLPGYGALFVNVNGQITLNPAMQGELTGLDALGSLGYMSIDPLASFSCTGKLANWSQGLLKDAAQYPADYRKFLAQPMLKGGQPDALYRQLARYQLELAMNNQLAQAQAAAGYAASGTDTSTEAQQSADSGNFAAVAPLVLNVEQQFRALGMDGSATQLTQCAHQYANSQLGRISLLADQSQLYQPAYLPASPAPDAYFFDLGSTPVVTDMLARQVSRVQVLVGYAQPFLTYLGQAGPSASNASANTANAAYWNNTASELRRYTQGKDPNSQPAVLDNLFLKLLPALQNDNCGEQLAAYSSPALGNDLFSNYRGQLMQGVQMHCKGERYAQAQNAFQPVASRFTRDLAGRYPFGTLDADDASLDAVKGFFTDYEGRRAALQKQVAGINDPYWKSVRQFLAQLDQVDAFLQGNVVPGTAPGDPAADPLLSLNVNFRALKPGANGSNQIIEMNLVSGAKGASFPNGGSTLDWQFGQPLVLDLSWAGLSPWRPSLAVGAPDLQVDGNTASFAAAGNWALLRMMERHRPSADPATDPRDATRSLLQFDVPVLDSSRAGSPATDTAHVFLGIRLSNVNAKTPTPLKLPAVFPISVPPLTPRP

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8806	-	-

Protein sequence: 1340 a.a.

						>T6CP259634 NC_012721:c884159-880140 [Burkholderia glumae BGR1] [TssM]
VDAILKSTAWLKAQTGWAPAAGTLKLVLGVLAALLALAVLAKLAGKLWARLGPLLKAPAVPPLCGCEPAAETRAKGWIER
ASLALDYLRTGREWRYATPWLLVLGQAGAGKTSLLESVGPQHTQPLGERQQSLALEGASWHAIDQSLVLDPLGKWPEAAA
SAMLDSGTADGADARRWLKFLDQLDALRPERALDGIVLVVCATTFLQRDPARLMAAAQNARQQLRTIEDRLEFALPVYVV
VTACDHVEGFGAFWRSQAPQRHTEMVGWSAPSQTFDTPPTQWGAQIFAAIGAQLRALQVETAAHCERIAADDADPMFLYP
LRFAQLQGPFEQWLSVVFQVSAWETTFFLRGVYFTGLVAEPGEPLEARDGPRRDVAFVRDLMLEKVLAEKHLARPTRAGV
WSRNLLIRRVQWACVIGFSLLLLAFAVRVWMIDAQIGRVIQALERMQQLQAPAPGAGCIAQAPVYQLIEQVAQIDADASS
WLLPASLFDSRLSHQSARRVIDTALRKVILPGLACQLSQRAYALGNEANRGISAGLGYTQALDQLQGFVQQADQYERNAA
RFQRLLGKTPYARERVPLPGFFDLVEYAYRTPVPPYLRAHAGLLPVTLGDLTERDFSGEIATPPQLRQSVGNHIVALAAQ
AGSLLQAELQSGLPLLEQLQARQQPILAHVRQFTHWLNWIRTAWLGSSAAANPILTVQNQLLASLQPLVADFGYPSYIQA
QVSAQFDAAHQYPLAMQTLNALTLPGYGALFVNVNGQITLNPAMQGELTGLDALGSLGYMSIDPLASFSCTGKLANWSQG
LLKDAAQYPADYRKFLAQPMLKGGQPDALYRQLARYQLELAMNNQLAQAQAAAGYAASGTDTSTEAQQSADSGNFAAVAP
LVLNVEQQFRALGMDGSATQLTQCAHQYANSQLGRISLLADQSQLYQPAYLPASPAPDAYFFDLGSTPVVTDMLARQVSR
VQVLVGYAQPFLTYLGQAGPSASNASANTANAAYWNNTASELRRYTQGKDPNSQPAVLDNLFLKLLPALQNDNCGEQLAA
YSSPALGNDLFSNYRGQLMQGVQMHCKGERYAQAQNAFQPVASRFTRDLAGRYPFGTLDADDASLDAVKGFFTDYEGRRA
ALQKQVAGINDPYWKSVRQFLAQLDQVDAFLQGNVVPGTAPGDPAADPLLSLNVNFRALKPGANGSNQIIEMNLVSGAKG
ASFPNGGSTLDWQFGQPLVLDLSWAGLSPWRPSLAVGAPDLQVDGNTASFAAAGNWALLRMMERHRPSADPATDPRDATR
SLLQFDVPVLDSSRAGSPATDTAHVFLGIRLSNVNAKTPTPLKLPAVFPISVPPLTPRP*					

Nucleotide sequence: 4020 bp

						>T6CP259634 NC_012721:c884159-880140 [Burkholderia glumae BGR1] [TssM]
GTGGACGCAATACTGAAATCGACCGCCTGGCTGAAGGCGCAGACCGGCTGGGCACCCGCTGCCGGCACGCTGAAGCTGGT
GCTGGGCGTGCTGGCCGCCCTGCTCGCGCTGGCCGTGCTGGCGAAGCTGGCCGGCAAGCTGTGGGCCAGGCTCGGGCCGC
TCCTGAAGGCGCCGGCCGTGCCGCCGCTGTGCGGCTGCGAGCCCGCCGCCGAGACCCGCGCGAAGGGCTGGATCGAACGC
GCCTCGCTTGCCCTCGACTATTTGCGCACCGGGCGCGAGTGGCGCTACGCGACGCCCTGGCTGCTGGTGCTCGGCCAGGC
CGGGGCCGGCAAGACCAGCCTGCTCGAATCGGTCGGCCCGCAGCACACGCAGCCGCTCGGCGAGCGGCAGCAGAGCCTCG
CGCTGGAAGGCGCGAGCTGGCACGCGATCGACCAGAGCCTGGTGCTCGATCCGCTCGGCAAGTGGCCCGAGGCGGCCGCC
AGCGCGATGCTCGACAGCGGCACGGCGGACGGCGCCGATGCGCGCCGCTGGCTGAAGTTCCTCGACCAGCTCGACGCGCT
GCGCCCCGAGCGCGCGCTCGACGGCATCGTGCTGGTGGTGTGCGCCACCACCTTCCTGCAGCGCGACCCGGCCCGCCTGA
TGGCCGCCGCGCAGAACGCGCGCCAGCAGCTGCGCACCATCGAGGACCGGCTCGAATTCGCACTGCCCGTCTATGTCGTG
GTCACCGCCTGCGATCACGTCGAGGGCTTCGGCGCGTTCTGGCGCTCGCAGGCGCCGCAGCGGCACACCGAGATGGTGGG
CTGGTCGGCGCCGAGCCAGACCTTCGACACGCCGCCCACCCAGTGGGGCGCGCAGATCTTCGCGGCGATCGGCGCCCAGC
TGCGCGCGCTGCAGGTGGAGACCGCCGCGCACTGCGAGCGGATCGCCGCCGACGACGCCGACCCGATGTTCCTCTACCCG
CTGCGCTTCGCGCAGTTGCAGGGGCCGTTCGAGCAGTGGCTGTCGGTGGTGTTCCAGGTATCGGCCTGGGAGACCACCTT
CTTCCTGCGCGGCGTCTATTTCACCGGCCTCGTCGCCGAGCCCGGCGAGCCGCTCGAAGCGCGCGACGGCCCGCGCCGCG
ACGTGGCGTTCGTGCGCGACCTGATGCTCGAGAAGGTGCTCGCGGAGAAGCATCTGGCGCGCCCGACGCGCGCCGGCGTG
TGGTCGCGCAACCTGCTGATCCGCCGCGTGCAGTGGGCCTGCGTGATCGGCTTCTCGCTGCTGCTGCTGGCGTTCGCCGT
GCGGGTGTGGATGATCGACGCGCAGATCGGCCGGGTGATCCAGGCGCTCGAGCGCATGCAGCAGCTGCAGGCACCCGCGC
CCGGCGCCGGCTGCATCGCGCAGGCGCCCGTCTATCAGCTGATCGAGCAGGTCGCGCAGATCGACGCCGACGCCAGCAGC
TGGCTGCTGCCGGCGTCGCTGTTCGATTCGCGGCTGAGCCACCAGAGCGCGCGGCGCGTGATCGACACCGCGTTGCGCAA
GGTGATCCTGCCGGGGCTCGCGTGCCAGCTGTCGCAACGCGCCTACGCGCTCGGCAACGAGGCCAATCGCGGCATCTCCG
CCGGGCTCGGCTACACCCAGGCGCTCGACCAGCTCCAAGGCTTCGTGCAGCAGGCCGACCAGTACGAGCGCAACGCGGCG
CGCTTCCAGCGCCTGCTGGGCAAGACCCCCTACGCGCGCGAGCGCGTGCCGTTGCCCGGCTTCTTCGATCTGGTCGAATA
CGCCTACCGCACGCCGGTGCCGCCGTATCTGCGCGCGCATGCCGGCCTGCTGCCCGTCACCCTCGGCGATCTCACCGAGC
GCGATTTCAGCGGCGAGATCGCCACCCCGCCGCAGCTCAGGCAGAGCGTCGGCAATCACATCGTCGCGCTGGCCGCGCAG
GCGGGCAGCCTGCTGCAGGCCGAACTGCAATCGGGCCTGCCGCTGCTCGAACAGCTGCAGGCCAGGCAGCAGCCGATCCT
CGCGCACGTCAGGCAGTTCACGCACTGGCTGAACTGGATCCGCACCGCGTGGCTCGGCTCCAGCGCCGCCGCCAACCCGA
TCCTGACGGTGCAGAACCAGCTGCTCGCGAGCCTGCAGCCGCTGGTCGCCGACTTCGGCTATCCGTCCTACATCCAGGCC
CAGGTCAGCGCGCAATTCGACGCCGCGCATCAGTATCCGCTCGCGATGCAGACGCTGAACGCGCTGACGCTGCCCGGCTA
CGGCGCGCTGTTCGTCAACGTCAACGGCCAGATCACGCTGAACCCGGCGATGCAGGGCGAGCTGACCGGCCTCGACGCGC
TCGGCAGCCTCGGCTACATGTCGATCGATCCGCTCGCCAGCTTCAGCTGCACGGGCAAGCTCGCCAACTGGAGCCAGGGC
CTGCTCAAGGACGCCGCCCAGTACCCGGCCGACTACCGGAAGTTCCTGGCCCAGCCGATGCTCAAGGGCGGCCAGCCCGA
CGCGCTCTATCGCCAGCTCGCGCGCTACCAGCTCGAACTGGCGATGAACAACCAGCTCGCGCAGGCGCAGGCCGCGGCCG
GCTACGCCGCGAGCGGCACCGACACCAGCACCGAGGCGCAGCAGAGCGCCGACAGCGGCAATTTCGCGGCCGTCGCGCCG
CTGGTGCTGAACGTGGAGCAGCAGTTCCGCGCGCTGGGCATGGACGGCAGCGCCACGCAGCTCACGCAGTGCGCGCACCA
GTACGCCAACAGCCAGCTCGGCCGCATCTCGCTGCTGGCCGACCAGAGCCAGCTGTATCAGCCGGCCTACCTGCCCGCCT
CGCCGGCCCCGGACGCCTACTTCTTCGATCTCGGCAGCACGCCGGTGGTGACCGACATGCTGGCGCGCCAGGTCAGCCGC
GTGCAGGTGCTGGTCGGCTACGCCCAGCCGTTCCTGACCTATCTCGGGCAGGCCGGGCCGTCGGCCTCGAACGCGAGCGC
CAACACCGCCAACGCGGCCTACTGGAACAACACCGCCAGCGAACTCAGGCGCTACACGCAGGGCAAGGACCCGAACTCGC
AGCCGGCCGTGCTCGACAACCTGTTCCTGAAGCTGCTGCCGGCGCTGCAGAACGACAACTGCGGCGAGCAGCTGGCCGCG
TACTCGTCGCCCGCGCTCGGCAACGATCTGTTCTCGAACTACCGCGGCCAGCTCATGCAGGGCGTGCAGATGCACTGCAA
GGGCGAGCGTTACGCGCAGGCGCAGAACGCGTTCCAGCCGGTGGCGAGCCGCTTCACGCGCGACCTGGCGGGCCGCTATC
CGTTCGGCACGCTCGACGCCGACGACGCCAGCCTCGACGCCGTGAAGGGCTTCTTCACCGACTACGAGGGCCGGCGCGCC
GCACTGCAGAAGCAGGTGGCGGGCATCAACGATCCGTACTGGAAGAGCGTGCGGCAGTTCCTCGCGCAGCTCGATCAGGT
GGACGCGTTCCTGCAGGGCAACGTGGTGCCGGGCACGGCGCCGGGCGACCCCGCCGCCGATCCGCTGCTGAGCCTGAACG
TCAACTTCCGCGCCCTCAAGCCCGGCGCGAACGGCAGCAACCAGATCATCGAGATGAACCTGGTGTCGGGCGCCAAGGGC
GCCTCGTTCCCGAACGGCGGCAGCACCCTGGACTGGCAGTTCGGGCAGCCGCTGGTGCTCGACCTGTCGTGGGCCGGCCT
CTCGCCGTGGCGGCCGTCGCTGGCCGTCGGCGCGCCCGATCTGCAGGTGGACGGCAACACCGCGTCGTTCGCGGCCGCCG
GCAACTGGGCGCTGCTGCGTATGATGGAGCGCCATCGGCCCAGCGCGGATCCGGCCACCGATCCGCGCGACGCCACGCGC
TCGCTGCTGCAGTTCGACGTGCCGGTGCTCGATTCGAGCCGCGCCGGCAGCCCGGCCACCGATACCGCACACGTATTCCT
CGGTATCCGGCTGTCGAACGTCAACGCGAAAACCCCGACGCCGCTGAAATTGCCGGCGGTATTCCCGATCTCGGTTCCTC
CCTTGACGCCACGACCATGA					