SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259633
Component protein type	TssA
T6SS ID (Type)	T6SS17510 (Type Unknown)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (382 a.a.); Nucleotide sequence (1146 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866 [2] Kim N, Han G, Jung H, et al. T6SS Accessory Proteins, Including DUF2169 Domain-Containing Protein and Pentapeptide Repeats Protein, Contribute to Bacterial Virulence in T6SS Group_5 of Burkholderia glumae BGR1. Plants (Basel). 2021 Dec 23;11(1). pii: 34. doi: 10.3390/plants11010034. PMID: 35009038

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS21475
Coordinate (Strand)	878998..880143 (-)
NCBI ID	WP_012733547.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	3.4e-28	25..147

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-381	MNPSLLADARQHFEQTLRISFDALLAPVEMYPPCGRPARECRSYSQINQERRHDDPSLPLGAWQRDLKRTDWLKVSHLVADMLLHDSKDMQLLAWLYEAQIKQYGFAGIAPTLVLLREMVSRYWNEIHPFGSEGDFEHRANLIRSISDKHLAALRLTPLLRLEGGKTYCWADWERAHRHERMRAGGHAELPAEGATLEELQGALNATASADFLTLRARLCDALQAIAEMSAALDPRFGNEAPSMGKTIELLETIRSLVDGELHTRGVRTEPEAPLQADATADDAAPAATEPHDTPGAAAAAPGGGPIASRADAYARLEEIAEYLMRQEPHSPVPYLVRRATLWGRMNTAELYQELFLRLGGQLNIFEMVGIENPGSPERQA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9805	-	-

Protein sequence: 382 a.a.

						>T6CP259633 NC_012721:c880143-878998 [Burkholderia glumae BGR1] [TssA]
MNPSLLADARQHFEQTLRISFDALLAPVEMYPPCGRPARECRSYSQINQERRHDDPSLPLGAWQRDLKRTDWLKVSHLVA
DMLLHDSKDMQLLAWLYEAQIKQYGFAGIAPTLVLLREMVSRYWNEIHPFGSEGDFEHRANLIRSISDKHLAALRLTPLL
RLEGGKTYCWADWERAHRHERMRAGGHAELPAEGATLEELQGALNATASADFLTLRARLCDALQAIAEMSAALDPRFGNE
APSMGKTIELLETIRSLVDGELHTRGVRTEPEAPLQADATADDAAPAATEPHDTPGAAAAAPGGGPIASRADAYARLEEI
AEYLMRQEPHSPVPYLVRRATLWGRMNTAELYQELFLRLGGQLNIFEMVGIENPGSPERQA*					

Nucleotide sequence: 1146 bp

						>T6CP259633 NC_012721:c880143-878998 [Burkholderia glumae BGR1] [TssA]
ATGAATCCATCGCTTCTTGCCGACGCCCGGCAGCATTTCGAACAGACCCTGCGGATTTCCTTCGACGCGCTGCTCGCGCC
GGTCGAGATGTACCCGCCGTGCGGACGGCCGGCGCGCGAGTGCCGCAGCTACAGCCAGATCAACCAGGAGCGGCGCCATG
ACGACCCGAGCCTGCCGCTCGGGGCCTGGCAGCGCGACCTGAAGCGCACCGACTGGCTCAAGGTCAGCCATCTGGTCGCG
GACATGCTGCTCCACGACAGCAAGGACATGCAGCTGCTGGCCTGGCTCTACGAGGCGCAGATCAAGCAATACGGCTTCGC
CGGCATCGCGCCGACGCTGGTGCTGCTGCGCGAGATGGTCTCGCGCTACTGGAACGAGATCCACCCGTTCGGGAGCGAGG
GCGATTTCGAGCATCGCGCCAACCTGATCCGCTCGATCTCCGACAAGCATCTCGCGGCGCTGCGGCTCACGCCGCTGCTG
CGCCTGGAGGGCGGCAAGACCTATTGCTGGGCCGACTGGGAGCGCGCGCATCGCCATGAGCGCATGCGCGCCGGCGGCCA
TGCCGAGCTGCCCGCCGAGGGCGCCACGCTGGAGGAGCTGCAGGGGGCGCTGAACGCCACCGCGAGCGCCGATTTCCTGA
CGCTGCGCGCGCGGCTCTGCGACGCGCTGCAGGCGATCGCCGAGATGAGCGCGGCGCTCGATCCGCGCTTCGGCAACGAG
GCGCCGAGCATGGGCAAGACCATCGAGCTGCTGGAGACGATCCGCTCGCTGGTCGACGGCGAACTGCACACGCGCGGCGT
GCGCACCGAGCCTGAAGCGCCGCTGCAGGCCGATGCCACGGCCGATGACGCCGCGCCCGCTGCAACCGAGCCGCACGACA
CGCCGGGCGCGGCCGCCGCCGCGCCTGGGGGCGGCCCGATCGCGAGCCGCGCCGACGCCTACGCGCGCCTCGAGGAAATC
GCCGAGTACCTGATGCGCCAGGAGCCGCACAGCCCGGTGCCGTACCTGGTGCGGCGCGCCACGCTGTGGGGCCGCATGAA
CACGGCCGAGCTGTATCAGGAGCTGTTCCTGAGGCTCGGCGGGCAGCTGAACATCTTCGAGATGGTGGGGATCGAGAATC
CCGGCAGCCCCGAACGGCAGGCATAA					