Detailed information of component protein
Summary
| Component ID | T6CP259628 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS17509  (Type i2) |
| Strain | Burkholderia glumae BGR1 |
| Replicon | chromosome II |
| Sequence | Protein sequence (244 a.a.); Nucleotide sequence (732 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BGLU_RS23275 |
| Coordinate (Strand) | 1416231..1416962 (+) |
| NCBI ID | WP_012733888.1 |
| RefSeq | NC_012721 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 1.7e-43 | 28..224 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-202 | MNTLTPDHNDVTPMDDLPGSSRVSGAGMRDLLRDTGLLVTTLAPGGRVQDAAGLRERCRQLITNFSDALARRGYPDDVRQDALIAQCGLLDETALRYLPAESRAAWQLKPLQVEQFNLHDAGERVIERLEARMREASPNLDLLECYSAILGMGFVGRYAREGEAKRTALVTSLNTQLETLRPSSARPFMTDRAGRRLSDWFY |
| Transmembrane helix | 203-225 | WLSPWAIAGLACVVALIVWMVWA |
| Outside | 226-243 | AALDVQIAHLLPVKGGQP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.8527 | - | - |
Protein sequence: 244 a.a.
>T6CP259628 NC_012721:1416231-1416962 [Burkholderia glumae BGR1] [TssL]
MNTLTPDHNDVTPMDDLPGSSRVSGAGMRDLLRDTGLLVTTLAPGGRVQDAAGLRERCRQLITNFSDALARRGYPDDVRQ
DALIAQCGLLDETALRYLPAESRAAWQLKPLQVEQFNLHDAGERVIERLEARMREASPNLDLLECYSAILGMGFVGRYAR
EGEAKRTALVTSLNTQLETLRPSSARPFMTDRAGRRLSDWFYWLSPWAIAGLACVVALIVWMVWAAALDVQIAHLLPVKG
GQP*
MNTLTPDHNDVTPMDDLPGSSRVSGAGMRDLLRDTGLLVTTLAPGGRVQDAAGLRERCRQLITNFSDALARRGYPDDVRQ
DALIAQCGLLDETALRYLPAESRAAWQLKPLQVEQFNLHDAGERVIERLEARMREASPNLDLLECYSAILGMGFVGRYAR
EGEAKRTALVTSLNTQLETLRPSSARPFMTDRAGRRLSDWFYWLSPWAIAGLACVVALIVWMVWAAALDVQIAHLLPVKG
GQP*
Nucleotide sequence: 732 bp
>T6CP259628 NC_012721:1416231-1416962 [Burkholderia glumae BGR1] [TssL]
ATGAACACCCTGACGCCAGATCATAACGACGTCACCCCGATGGACGATCTGCCTGGCTCCAGCCGTGTGTCTGGCGCAGG
TATGCGTGACTTGCTGCGCGATACGGGCCTTCTCGTGACGACACTTGCGCCTGGAGGCCGGGTTCAGGACGCTGCCGGGC
TCCGGGAACGTTGCCGGCAGCTCATCACGAATTTTTCGGACGCGTTGGCGCGACGCGGCTATCCGGACGACGTCCGACAG
GATGCACTTATCGCACAGTGTGGCCTGCTAGACGAGACCGCACTGCGCTATTTGCCGGCCGAAAGCCGCGCGGCCTGGCA
ATTGAAGCCTTTACAGGTCGAACAGTTCAACCTGCATGATGCAGGCGAACGCGTAATCGAACGTCTGGAAGCGCGTATGC
GCGAAGCCTCACCCAACCTCGACCTGCTCGAGTGTTACTCGGCGATCCTCGGCATGGGATTCGTGGGACGCTATGCCCGC
GAAGGCGAAGCGAAGCGCACGGCGCTGGTCACATCACTGAACACCCAGTTGGAGACGCTTCGGCCTTCGTCGGCGCGGCC
CTTCATGACCGACCGCGCGGGAAGACGGCTCTCCGACTGGTTCTACTGGCTTTCGCCGTGGGCAATAGCAGGTCTGGCCT
GCGTCGTCGCGCTAATCGTCTGGATGGTCTGGGCAGCGGCGCTCGATGTGCAGATCGCGCATCTGCTGCCGGTAAAGGGT
GGCCAGCCGTGA
ATGAACACCCTGACGCCAGATCATAACGACGTCACCCCGATGGACGATCTGCCTGGCTCCAGCCGTGTGTCTGGCGCAGG
TATGCGTGACTTGCTGCGCGATACGGGCCTTCTCGTGACGACACTTGCGCCTGGAGGCCGGGTTCAGGACGCTGCCGGGC
TCCGGGAACGTTGCCGGCAGCTCATCACGAATTTTTCGGACGCGTTGGCGCGACGCGGCTATCCGGACGACGTCCGACAG
GATGCACTTATCGCACAGTGTGGCCTGCTAGACGAGACCGCACTGCGCTATTTGCCGGCCGAAAGCCGCGCGGCCTGGCA
ATTGAAGCCTTTACAGGTCGAACAGTTCAACCTGCATGATGCAGGCGAACGCGTAATCGAACGTCTGGAAGCGCGTATGC
GCGAAGCCTCACCCAACCTCGACCTGCTCGAGTGTTACTCGGCGATCCTCGGCATGGGATTCGTGGGACGCTATGCCCGC
GAAGGCGAAGCGAAGCGCACGGCGCTGGTCACATCACTGAACACCCAGTTGGAGACGCTTCGGCCTTCGTCGGCGCGGCC
CTTCATGACCGACCGCGCGGGAAGACGGCTCTCCGACTGGTTCTACTGGCTTTCGCCGTGGGCAATAGCAGGTCTGGCCT
GCGTCGTCGCGCTAATCGTCTGGATGGTCTGGGCAGCGGCGCTCGATGTGCAGATCGCGCATCTGCTGCCGGTAAAGGGT
GGCCAGCCGTGA