Detailed information of component protein


Summary

Component ID T6CP259622
Component protein type TssM
T6SS ID (Type) T6SS17509 experimental (Type i2)
Strain Burkholderia glumae BGR1
Replicon chromosome II
Sequence Protein sequence (1175 a.a.); Nucleotide sequence (3525 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) BGLU_RS23225
Coordinate (Strand) 1398818..1402342 (-)
NCBI ID WP_012733879.1
RefSeq NC_012721
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 3.9e-66 414..733
IcmF_C PF06744.14 9.5e-20 1002..1093
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MKVSKI
Transmembrane helix 7-26 SGYWVVVIAATIGAAVIAWF
Outside 27-35 KGSAFDPSP
Transmembrane helix 36-57 VHRIVAVVFIGVLALALRGQLA
Inside 58-360 GGWQAVARHSPGRLGTRYDRDPIVRVGVTQKEEGENLNAYRGANLCDALRDRHGWRWRYRERWVLVAGDLPLVKRLAPGLVEAGYLITGDAVLLYAQQTRDTLETDWLDQIRRLRRRRPVDAIVVVTCNRSSANAPFDTDALAQQLARHARALRWAAPAYLLNVTDFGSETSSPDEAIGFTWANARISADQIDASLQDLAYNLADAGVVRLAKDAADRYPAELSQHISTLHGPLSGLVLQTAQSRIWRQAIHGLLFAPLVEERERAPLLPTDANDDEPAGEPRHRTIWQTVAEHSRRIHGRRV
Transmembrane helix 361-383 GFSPSTTAAWITTGLVGCWIAGT
Outside 384-1174 MLSGFVNRATIRNVADTIARLSTVQDRTQALQALNGLDRQIDTLEVHQRDGAPWSTRFGLNRDGALLDALWPGYANAASRILVAPIRQKLEARLRKLASLSDAQIASGGNAQVQAVYDTLKAYLMLARPERAVAAFLTPQLVATAAPVRPANSPLSSGAWEDLRQHTIAFLANHLGRGRWQAMAPDLGLVASTRQTVIGVRGIQNSTDAVYQQILDDAKAKYPPVSLATLLGDTTSRGLFDTTAAVPGVFTRAAWDERISKAIDKASEQQNVAGDWVLSDVKATQSAPSRLKAQLRQRYFDDYGRAWALFLNSLRWQAAPTLSATADQLTLLGDPQRSPLVALMNAIVYQAGAGANAQSLADNLISKAQQLVGGAEKDPSKQTQPPLAPLAKAFGPILRLTGSDLVLGAAASGKAAAPLAATGDLSLARYLERVTAMRLKASQIVSGADPDAMARQAAQAVLQGRTSDIAESRDYASRLAASLGEQWSGFGELFRAPFDQAWQVVVQPAASSLNDMWRTAIVADWTRTFGGRYPFADSDNDASLPEMARFMRPDNGVIAQFVTTQLAGVVERQGDRWVAVQGADHGALTIDPGFLASLNQLTRVSTMLFPSGDAHLRYELRPEPTPGITDMKFVLSGRELRYFNQKQEWTPFEWPGQSLENLSHIEWQTEQGGLRTALDSQGRFGLIRLLERAKVSQQDSARYLLTWTPDTSLGLPLRVQLRSEVGSGPLEVLELRHYTLPARIFVTGATKAEPTPSAIGPPPLPPAAISAAKHAAVPLPDGASGPLPEVE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9777 - -


  Protein sequence: 1175 a.a.    

>T6CP259622 NC_012721:c1402342-1398818 [Burkholderia glumae BGR1] [TssM]
MKVSKISGYWVVVIAATIGAAVIAWFKGSAFDPSPVHRIVAVVFIGVLALALRGQLAGGWQAVARHSPGRLGTRYDRDPI
VRVGVTQKEEGENLNAYRGANLCDALRDRHGWRWRYRERWVLVAGDLPLVKRLAPGLVEAGYLITGDAVLLYAQQTRDTL
ETDWLDQIRRLRRRRPVDAIVVVTCNRSSANAPFDTDALAQQLARHARALRWAAPAYLLNVTDFGSETSSPDEAIGFTWA
NARISADQIDASLQDLAYNLADAGVVRLAKDAADRYPAELSQHISTLHGPLSGLVLQTAQSRIWRQAIHGLLFAPLVEER
ERAPLLPTDANDDEPAGEPRHRTIWQTVAEHSRRIHGRRVGFSPSTTAAWITTGLVGCWIAGTMLSGFVNRATIRNVADT
IARLSTVQDRTQALQALNGLDRQIDTLEVHQRDGAPWSTRFGLNRDGALLDALWPGYANAASRILVAPIRQKLEARLRKL
ASLSDAQIASGGNAQVQAVYDTLKAYLMLARPERAVAAFLTPQLVATAAPVRPANSPLSSGAWEDLRQHTIAFLANHLGR
GRWQAMAPDLGLVASTRQTVIGVRGIQNSTDAVYQQILDDAKAKYPPVSLATLLGDTTSRGLFDTTAAVPGVFTRAAWDE
RISKAIDKASEQQNVAGDWVLSDVKATQSAPSRLKAQLRQRYFDDYGRAWALFLNSLRWQAAPTLSATADQLTLLGDPQR
SPLVALMNAIVYQAGAGANAQSLADNLISKAQQLVGGAEKDPSKQTQPPLAPLAKAFGPILRLTGSDLVLGAAASGKAAA
PLAATGDLSLARYLERVTAMRLKASQIVSGADPDAMARQAAQAVLQGRTSDIAESRDYASRLAASLGEQWSGFGELFRAP
FDQAWQVVVQPAASSLNDMWRTAIVADWTRTFGGRYPFADSDNDASLPEMARFMRPDNGVIAQFVTTQLAGVVERQGDRW
VAVQGADHGALTIDPGFLASLNQLTRVSTMLFPSGDAHLRYELRPEPTPGITDMKFVLSGRELRYFNQKQEWTPFEWPGQ
SLENLSHIEWQTEQGGLRTALDSQGRFGLIRLLERAKVSQQDSARYLLTWTPDTSLGLPLRVQLRSEVGSGPLEVLELRH
YTLPARIFVTGATKAEPTPSAIGPPPLPPAAISAAKHAAVPLPDGASGPLPEVE*

  Nucleotide sequence: 3525 bp    

>T6CP259622 NC_012721:c1402342-1398818 [Burkholderia glumae BGR1] [TssM]
ATGAAGGTAAGCAAAATCTCCGGCTACTGGGTTGTCGTGATCGCTGCGACGATCGGCGCGGCGGTCATTGCCTGGTTCAA
AGGCTCGGCTTTTGATCCGTCTCCGGTTCATCGGATCGTCGCAGTAGTGTTCATCGGCGTGCTCGCGCTGGCGCTACGCG
GACAGTTGGCAGGTGGGTGGCAGGCCGTAGCCCGACACTCGCCCGGAAGATTGGGCACCCGGTACGACCGCGATCCGATC
GTCCGAGTTGGCGTCACGCAAAAGGAGGAAGGGGAAAACCTCAATGCGTACCGCGGCGCGAATCTCTGCGACGCGCTGCG
CGACCGCCACGGCTGGCGCTGGCGCTATCGCGAACGGTGGGTACTCGTCGCCGGTGACCTGCCGCTGGTGAAACGCCTGG
CGCCGGGACTCGTCGAGGCCGGGTATCTGATTACCGGCGACGCGGTCTTGCTGTACGCGCAGCAAACCCGCGACACACTC
GAAACGGACTGGCTCGATCAAATCCGCCGCCTGCGCCGCCGTCGTCCGGTCGACGCGATCGTGGTCGTGACGTGCAATCG
CAGTTCAGCCAATGCTCCGTTCGACACCGACGCGCTTGCCCAACAGCTCGCGCGCCATGCCCGCGCGCTGCGCTGGGCCG
CGCCCGCGTATCTGCTCAACGTCACCGATTTCGGCAGCGAAACGTCGAGTCCCGACGAGGCGATCGGGTTCACGTGGGCG
AACGCGCGCATCAGCGCGGATCAGATCGACGCATCGTTGCAAGACCTCGCTTACAACCTGGCCGATGCCGGCGTCGTGCG
GCTGGCGAAGGATGCCGCCGACCGCTATCCCGCCGAGCTCTCGCAGCATATTTCGACGCTGCACGGCCCACTGTCCGGGT
TGGTGCTGCAGACTGCCCAATCCCGCATCTGGCGGCAGGCGATTCACGGGCTGCTTTTCGCACCGCTCGTCGAGGAACGG
GAGCGGGCACCGTTGTTGCCCACCGACGCAAACGACGACGAGCCGGCGGGTGAACCTCGACATCGCACGATCTGGCAGAC
CGTCGCCGAACACAGCCGCAGAATCCACGGCCGCCGCGTCGGCTTCTCGCCGTCGACGACAGCGGCCTGGATCACGACCG
GCCTGGTCGGCTGCTGGATCGCCGGCACGATGCTGTCGGGATTCGTCAACCGTGCAACGATCCGGAACGTGGCCGATACC
ATTGCGCGGCTGTCGACCGTTCAGGATCGCACGCAGGCCTTGCAAGCGCTCAACGGCCTCGACCGGCAGATCGATACGCT
CGAAGTCCATCAGCGCGACGGCGCGCCGTGGAGCACGCGCTTCGGCCTGAACCGCGACGGCGCGCTGCTCGATGCGCTAT
GGCCGGGCTATGCCAATGCGGCGAGCCGCATCCTCGTGGCGCCGATCCGCCAGAAGCTCGAGGCGCGCCTGCGCAAGCTT
GCCTCCCTGTCGGATGCCCAGATCGCCAGCGGCGGCAATGCGCAGGTGCAGGCCGTCTACGACACGCTGAAGGCTTACCT
GATGCTCGCCAGGCCAGAACGGGCGGTCGCCGCATTCCTGACTCCGCAACTCGTCGCGACGGCCGCGCCCGTGCGCCCCG
CGAACTCGCCGCTGTCGTCGGGGGCGTGGGAAGACCTGCGCCAGCACACGATCGCGTTCCTGGCGAATCATCTGGGCCGC
GGAAGGTGGCAGGCGATGGCGCCGGATCTGGGGCTGGTGGCCTCGACGCGCCAGACGGTGATCGGCGTGCGCGGCATCCA
GAACTCGACCGATGCGGTCTATCAGCAGATCCTCGACGACGCGAAGGCCAAGTATCCGCCTGTCTCGCTCGCGACGCTGC
TGGGCGACACGACCAGCCGGGGCCTATTCGATACGACGGCGGCCGTGCCGGGTGTGTTCACACGCGCCGCCTGGGACGAG
CGGATTTCGAAAGCGATCGACAAAGCGAGCGAGCAGCAAAACGTGGCGGGCGACTGGGTGCTCTCCGACGTCAAGGCGAC
GCAGTCCGCACCGTCAAGGCTGAAGGCCCAGCTGCGCCAGCGCTACTTCGACGATTATGGACGGGCCTGGGCGCTGTTCC
TCAACAGCCTGCGCTGGCAAGCGGCGCCGACGCTGTCCGCCACGGCCGACCAGCTCACGCTGCTGGGCGACCCGCAACGC
TCGCCGCTCGTCGCGCTGATGAACGCGATCGTCTACCAGGCGGGTGCCGGCGCGAATGCGCAGTCGCTCGCCGACAATCT
GATCAGCAAGGCGCAGCAGCTCGTCGGCGGCGCCGAGAAGGATCCGTCGAAGCAGACCCAGCCGCCGCTCGCGCCGCTTG
CCAAGGCGTTCGGGCCGATCCTGCGCCTGACCGGCAGCGATCTGGTGCTAGGCGCAGCTGCGAGCGGCAAGGCCGCCGCA
CCGCTTGCCGCGACCGGCGACTTGAGCCTCGCACGATACCTGGAGCGCGTTACCGCCATGCGCCTGAAGGCCTCGCAGAT
CGTCTCCGGTGCCGATCCGGACGCGATGGCGCGTCAGGCGGCGCAGGCGGTCCTGCAAGGCAGGACGTCCGACATCGCCG
AGAGCCGCGACTACGCCAGCCGCCTCGCCGCGAGCCTGGGCGAGCAGTGGTCCGGGTTTGGCGAGCTGTTCCGCGCGCCG
TTCGACCAGGCCTGGCAAGTTGTCGTGCAGCCGGCGGCGTCGAGCCTGAACGACATGTGGCGCACCGCAATCGTGGCGGA
CTGGACCAGGACCTTCGGCGGCCGCTATCCGTTCGCGGACTCCGACAATGACGCGTCGCTGCCCGAAATGGCGCGCTTCA
TGCGGCCCGATAACGGCGTGATCGCGCAGTTCGTCACCACTCAGCTTGCCGGCGTGGTCGAGCGGCAGGGTGACCGATGG
GTGGCGGTGCAGGGGGCCGACCACGGTGCGCTGACGATCGATCCCGGCTTCCTCGCCAGCCTGAACCAGCTCACCCGTGT
CTCGACCATGCTGTTCCCGTCCGGGGACGCGCACCTGCGATACGAGTTGCGGCCGGAGCCGACGCCGGGCATCACGGACA
TGAAGTTCGTGCTGTCCGGGCGCGAGCTGCGCTACTTCAACCAGAAGCAGGAGTGGACGCCGTTCGAGTGGCCCGGGCAG
TCGCTGGAGAACCTGTCGCACATCGAATGGCAGACCGAGCAGGGCGGGCTGCGCACGGCGCTCGATTCGCAGGGCCGGTT
CGGCCTGATCCGGCTGCTGGAACGTGCGAAAGTCTCGCAGCAGGACAGCGCGCGCTATCTGCTGACCTGGACGCCGGACA
CGAGCCTCGGGCTGCCGCTGCGCGTGCAACTGCGCAGCGAGGTCGGCTCGGGGCCGCTGGAGGTGCTGGAACTGCGGCAC
TACACGCTGCCCGCCCGGATCTTCGTCACGGGCGCGACGAAGGCGGAGCCGACGCCGTCGGCCATCGGTCCGCCACCATT
GCCGCCGGCCGCGATCTCGGCGGCCAAACATGCCGCGGTGCCGCTGCCTGATGGCGCATCGGGTCCATTGCCGGAGGTCG
AATGA