Detailed information of component protein
Summary
| Component ID | T6CP259618 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS17509  (Type i2) |
| Strain | Burkholderia glumae BGR1 |
| Replicon | chromosome II |
| Sequence | Protein sequence (186 a.a.); Nucleotide sequence (558 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | BGLU_RS23205 |
| Coordinate (Strand) | 1393584..1394141 (-) |
| NCBI ID | WP_012733875.1 |
| RefSeq | NC_012721 |
| Uniprot ID | - |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.1e-30 | 51..167 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-185 | MPDRGVPMPIRVTLSILASTLLLSACGAWQAVSDASSSTYHAVFFKQTKVLNVDLSAREALNPDDAGRPTSVAVRIYQLKDRKIFDQASYEDLLRNDRTVLSQDLLTSMASVINPGASASFTQRMQADTKYVAIVAFYRNPGNGDGWKYVIEKKKLDADKPLKLELVDQLLIDARGTQSPASYIP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9885 | pos: 25-26. LSA-CG. | MPDRGVPMPIRVTLSILASTLLLSA |
Protein sequence: 186 a.a.
>T6CP259618 NC_012721:c1394141-1393584 [Burkholderia glumae BGR1] [TssJ]
MPDRGVPMPIRVTLSILASTLLLSACGAWQAVSDASSSTYHAVFFKQTKVLNVDLSAREALNPDDAGRPTSVAVRIYQLK
DRKIFDQASYEDLLRNDRTVLSQDLLTSMASVINPGASASFTQRMQADTKYVAIVAFYRNPGNGDGWKYVIEKKKLDADK
PLKLELVDQLLIDARGTQSPASYIP*
MPDRGVPMPIRVTLSILASTLLLSACGAWQAVSDASSSTYHAVFFKQTKVLNVDLSAREALNPDDAGRPTSVAVRIYQLK
DRKIFDQASYEDLLRNDRTVLSQDLLTSMASVINPGASASFTQRMQADTKYVAIVAFYRNPGNGDGWKYVIEKKKLDADK
PLKLELVDQLLIDARGTQSPASYIP*
Nucleotide sequence: 558 bp
>T6CP259618 NC_012721:c1394141-1393584 [Burkholderia glumae BGR1] [TssJ]
ATGCCGGATCGAGGAGTTCCGATGCCTATTCGTGTCACTCTTTCAATTCTTGCGTCCACCTTGCTGCTGTCGGCCTGCGG
CGCATGGCAGGCTGTCTCGGACGCCTCGTCGAGCACGTATCACGCCGTGTTCTTCAAGCAGACGAAGGTGCTCAACGTCG
ATCTTTCCGCCCGCGAAGCCTTGAATCCGGACGACGCCGGCCGCCCGACGTCGGTTGCCGTGCGCATATACCAGCTCAAG
GACCGGAAGATATTCGACCAGGCATCTTACGAGGACCTACTGAGGAACGACCGGACCGTGCTTTCTCAGGATCTGCTAAC
GAGCATGGCATCCGTGATCAACCCGGGCGCGTCCGCAAGCTTTACACAGCGGATGCAGGCCGATACGAAGTACGTGGCCA
TCGTTGCGTTCTATCGAAATCCGGGCAACGGCGACGGCTGGAAATACGTGATCGAGAAAAAGAAGCTCGACGCTGACAAG
CCGCTTAAACTGGAACTGGTTGACCAGTTGCTCATTGACGCTCGCGGAACCCAAAGCCCGGCGTCATACATCCCGTGA
ATGCCGGATCGAGGAGTTCCGATGCCTATTCGTGTCACTCTTTCAATTCTTGCGTCCACCTTGCTGCTGTCGGCCTGCGG
CGCATGGCAGGCTGTCTCGGACGCCTCGTCGAGCACGTATCACGCCGTGTTCTTCAAGCAGACGAAGGTGCTCAACGTCG
ATCTTTCCGCCCGCGAAGCCTTGAATCCGGACGACGCCGGCCGCCCGACGTCGGTTGCCGTGCGCATATACCAGCTCAAG
GACCGGAAGATATTCGACCAGGCATCTTACGAGGACCTACTGAGGAACGACCGGACCGTGCTTTCTCAGGATCTGCTAAC
GAGCATGGCATCCGTGATCAACCCGGGCGCGTCCGCAAGCTTTACACAGCGGATGCAGGCCGATACGAAGTACGTGGCCA
TCGTTGCGTTCTATCGAAATCCGGGCAACGGCGACGGCTGGAAATACGTGATCGAGAAAAAGAAGCTCGACGCTGACAAG
CCGCTTAAACTGGAACTGGTTGACCAGTTGCTCATTGACGCTCGCGGAACCCAAAGCCCGGCGTCATACATCCCGTGA