SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259612
Component protein type	TssA
T6SS ID (Type)	T6SS17508 (Type i3)
Strain	Burkholderia glumae BGR1
Replicon	chromosome II
Sequence	Protein sequence (355 a.a.); Nucleotide sequence (1065 bp)
Reference	[1] Kim N, Kim JJ, Kim I, et al. Type VI secretion systems of plant-pathogenic Burkholderia glumae BGR1 play a functionally distinct role in interspecies interactions and virulence. Mol Plant Pathol. 2020 Aug;21(8):1055-1069. doi: 10.1111/mpp.12966. Epub 2020 Jul 9. PMID: 32643866

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BGLU_RS18710
Coordinate (Strand)	178418..179482 (-)
NCBI ID	WP_012733040.1
RefSeq	NC_012721
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.5e-32	16..139

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-354	MTLETPSPATRYTDLLEPVSSDAPCGADLEYDPAFVMLQAAVAPRVEAQYGDFVELPPPANWAEIERECRALLLRTKDIRVAVILLRCRVRLEGATGLSDALAFLQSLFERYGEALHPLPVADGERDPVAYVNAIAALADHDGVLSDVRDIALPRAAGLKLHLRDVDKALATPRPKDALAPESVVRALRELWNRRDAVLVSLADAQQRLDTLTAWCAEAFGADAPDLGALARLLQPFAPVRLNGEGSAAPDMPAAPQAAGNDARADAPPSANAAATAQPGANDGGTVGALPDDVDRWAALATLRDTRLWFERKEPSSPVIVLLRQCERMVGKRFSELVHLIPADLLAKWDAIDD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9821	-	-

Protein sequence: 355 a.a.

						>T6CP259612 NC_012721:c179482-178418 [Burkholderia glumae BGR1] [TssA]
MTLETPSPATRYTDLLEPVSSDAPCGADLEYDPAFVMLQAAVAPRVEAQYGDFVELPPPANWAEIERECRALLLRTKDIR
VAVILLRCRVRLEGATGLSDALAFLQSLFERYGEALHPLPVADGERDPVAYVNAIAALADHDGVLSDVRDIALPRAAGLK
LHLRDVDKALATPRPKDALAPESVVRALRELWNRRDAVLVSLADAQQRLDTLTAWCAEAFGADAPDLGALARLLQPFAPV
RLNGEGSAAPDMPAAPQAAGNDARADAPPSANAAATAQPGANDGGTVGALPDDVDRWAALATLRDTRLWFERKEPSSPVI
VLLRQCERMVGKRFSELVHLIPADLLAKWDAIDD*					

Nucleotide sequence: 1065 bp

						>T6CP259612 NC_012721:c179482-178418 [Burkholderia glumae BGR1] [TssA]
ATGACACTCGAGACGCCCTCTCCCGCCACCCGTTACACCGATCTGCTCGAACCGGTTTCCTCCGACGCACCGTGCGGCGC
CGACCTCGAATACGACCCGGCGTTCGTGATGCTGCAAGCGGCCGTGGCGCCACGCGTCGAGGCTCAGTATGGCGACTTCG
TCGAACTGCCGCCGCCTGCCAACTGGGCCGAGATCGAACGCGAATGCCGCGCCCTGCTGCTGCGCACCAAAGACATCCGG
GTCGCGGTGATCCTGCTGCGCTGCCGCGTCCGGCTGGAAGGCGCGACCGGACTGAGCGATGCGCTGGCGTTTCTCCAGAG
CCTGTTCGAGCGCTACGGCGAGGCTCTGCACCCGCTGCCGGTGGCAGACGGCGAGCGCGACCCAGTAGCCTATGTCAACG
CGATCGCCGCGCTGGCCGACCACGACGGCGTGCTCAGCGACGTGCGCGACATCGCGCTGCCGAGAGCCGCGGGTCTCAAG
CTGCATCTGCGCGACGTCGACAAGGCGCTGGCCACGCCTCGCCCCAAGGACGCGCTGGCGCCCGAATCGGTCGTGCGGGC
GCTGCGCGAGCTGTGGAACCGGCGCGACGCGGTGCTGGTTTCCCTGGCCGACGCGCAGCAGCGGCTCGACACGCTCACGG
CCTGGTGCGCCGAGGCATTCGGCGCCGACGCGCCTGACCTTGGCGCGCTGGCGCGCCTCCTTCAACCGTTCGCGCCGGTG
CGGCTGAACGGCGAAGGCTCTGCGGCGCCCGACATGCCGGCGGCCCCGCAGGCCGCCGGCAACGACGCGCGGGCCGATGC
GCCGCCTAGCGCAAACGCGGCGGCGACGGCCCAGCCGGGCGCGAACGATGGGGGCACCGTGGGTGCGCTGCCGGACGACG
TCGATCGCTGGGCCGCACTCGCGACGCTGCGCGACACGCGCCTATGGTTCGAGCGGAAGGAGCCGAGCAGCCCCGTGATC
GTGCTGTTGAGACAGTGCGAGCGGATGGTCGGCAAGCGCTTCTCCGAACTGGTCCATCTGATCCCGGCCGACTTGCTGGC
CAAGTGGGATGCGATCGACGACTGA					