Detailed information of component protein
Summary
Component ID | T6CP259609 |
Component protein type | TssM |
T6SS ID (Type) | T6SS17508 (Type i3) |
Strain | Burkholderia glumae BGR1 |
Replicon | chromosome II |
Sequence | Protein sequence (1366 a.a.); Nucleotide sequence (4098 bp) |
Reference |
External database links
Locus tag (Gene) | BGLU_RS18680 |
Coordinate (Strand) | 163266..167363 (+) |
NCBI ID | WP_012733033.1 |
RefSeq | NC_012721 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 8.3e-80 | 274..551 |
IcmF-related | PF06761.14 | 1.2e-76 | 618..918 |
IcmF_C | PF06744.14 | 5.9e-27 | 1198..1301 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Inside | 1-15 | MSHLNRFFKFLISRQ |
Transmembrane helix | 16-38 | TLVFVAIVAVAAAIWFIGPLIAF |
Outside | 39-47 | GDYRPLATV |
Transmembrane helix | 48-70 | LARALSIVTLLAMLLFCLLGWSL |
Inside | 71-71 | G |
Transmembrane helix | 72-94 | VIGAALLCLAIWYLGPLFMFGSV |
Outside | 95-103 | RPLASTGVR |
Transmembrane helix | 104-123 | VLLIAVLVACYAAYGLYRLW |
Inside | 124-545 | KAMRSNDALLQRILNPFSTQASDDAPAREQIRAVNSVVTKAIDKVKQLRGGAFGLRRLLESQRYLYALPWYLVMGSPGAGKTTMILNSGLEFPDADQMVAASLGHHAETVNCDWWFGNDAILIDTAGRYLMHDMPARADEPDHAAGAVGSANAAEWRGFLALLRKRRPRAPVNGVLLVVSVEELLTCSPSELKLLAAAMRARLGELKHDLRIRYPAYLVVTKLDLLPGFLEYFQSLTAESRAQILGFTLPYHRDAPEERRDALRLACIEELRELEQGFEDGLNARLQEEYELDRRRRLYALPAEFRSLCAILVEFVASVFVDSKYDDTELNGVLRGVYFSSAAQTHDVVPADRDTLLQRLRRGLAARFGADAAAVSAPAPDGAAPVEYRGYFLRNLFQRVIVAEGHLVRPNLRWEIRFRALR |
Transmembrane helix | 546-565 | VVGHLLSIAVAAWLVYGMAG |
Outside | 566-1365 | SFQRNRDYLAAIDHQTNALAARVQAQRRTPKPGDTGALLSASLDLPRFGELDLDRPGIAWRYGLYAAPGVVDASSRTYAALLDQMLLPPVVARIATVLDGEVRAQNADEVYKTLPVYLMLYDPSHYDAKEVKAWVMRDWEQAGSVAMLGDRDVVAGHLDALFRDGVPVTPATPMDGALIKRARELLGRNSASGRLYERAMKAMEPDAPENITLARAGGPQALSVFAMAGDPALAQGVPGLYTYEGYHEVFTRRLPEFLEQAHERDAWLMGRGDRSSTWGRAGRVLRAGERALADEIRRQYLIDYGNYWQRFLDGVRLASGGAAPALDLQMLRTLSAPDSPLVRLARTAARETSLATPDTQDGPSLAGSAAAIASRRSRAASAVLAIGGATSTINRLARRRMEHDLVEQRFAALRAVVTGEADTDGGKLVRAWSASASLSSLPSLPSPGGGGPMQLDGIVSLIGERYTRLVLAGDVLATDSMPPADDLGATMRLEAEKLPAPFRAVLAGLAERSTQEVNRKMGALLGSRLEANVGDPCRSLIEGRYSFADSAQEVSIADFNRVFAAKGLLDKFFRETLADHVDTGSHPWRYRPAAPGMPPMRGPSLEPFERAAAIRRAFFLGPGASRLVWKLGIRVVSLDPDITELRLDIDGQSLRYAHGPVTTFPVTWPGPRGGSMAGITAEPQVSAQSSTLGAEGPWALFRLFDRGRLVPTSDTSRYTEDFDFDGRHAVLELNTGGRAGGEPIGLLKGFRCPGFGGVTGGMTAAATSAPRSAAAAPAGEADDAGEGTRGSEPPTEAAFD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9897 | - | - |
Protein sequence: 1366 a.a.
>T6CP259609 NC_012721:163266-167363 [Burkholderia glumae BGR1] [TssM]
MSHLNRFFKFLISRQTLVFVAIVAVAAAIWFIGPLIAFGDYRPLATVLARALSIVTLLAMLLFCLLGWSLGVIGAALLCL
AIWYLGPLFMFGSVRPLASTGVRVLLIAVLVACYAAYGLYRLWKAMRSNDALLQRILNPFSTQASDDAPAREQIRAVNSV
VTKAIDKVKQLRGGAFGLRRLLESQRYLYALPWYLVMGSPGAGKTTMILNSGLEFPDADQMVAASLGHHAETVNCDWWFG
NDAILIDTAGRYLMHDMPARADEPDHAAGAVGSANAAEWRGFLALLRKRRPRAPVNGVLLVVSVEELLTCSPSELKLLAA
AMRARLGELKHDLRIRYPAYLVVTKLDLLPGFLEYFQSLTAESRAQILGFTLPYHRDAPEERRDALRLACIEELRELEQG
FEDGLNARLQEEYELDRRRRLYALPAEFRSLCAILVEFVASVFVDSKYDDTELNGVLRGVYFSSAAQTHDVVPADRDTLL
QRLRRGLAARFGADAAAVSAPAPDGAAPVEYRGYFLRNLFQRVIVAEGHLVRPNLRWEIRFRALRVVGHLLSIAVAAWLV
YGMAGSFQRNRDYLAAIDHQTNALAARVQAQRRTPKPGDTGALLSASLDLPRFGELDLDRPGIAWRYGLYAAPGVVDASS
RTYAALLDQMLLPPVVARIATVLDGEVRAQNADEVYKTLPVYLMLYDPSHYDAKEVKAWVMRDWEQAGSVAMLGDRDVVA
GHLDALFRDGVPVTPATPMDGALIKRARELLGRNSASGRLYERAMKAMEPDAPENITLARAGGPQALSVFAMAGDPALAQ
GVPGLYTYEGYHEVFTRRLPEFLEQAHERDAWLMGRGDRSSTWGRAGRVLRAGERALADEIRRQYLIDYGNYWQRFLDGV
RLASGGAAPALDLQMLRTLSAPDSPLVRLARTAARETSLATPDTQDGPSLAGSAAAIASRRSRAASAVLAIGGATSTINR
LARRRMEHDLVEQRFAALRAVVTGEADTDGGKLVRAWSASASLSSLPSLPSPGGGGPMQLDGIVSLIGERYTRLVLAGDV
LATDSMPPADDLGATMRLEAEKLPAPFRAVLAGLAERSTQEVNRKMGALLGSRLEANVGDPCRSLIEGRYSFADSAQEVS
IADFNRVFAAKGLLDKFFRETLADHVDTGSHPWRYRPAAPGMPPMRGPSLEPFERAAAIRRAFFLGPGASRLVWKLGIRV
VSLDPDITELRLDIDGQSLRYAHGPVTTFPVTWPGPRGGSMAGITAEPQVSAQSSTLGAEGPWALFRLFDRGRLVPTSDT
SRYTEDFDFDGRHAVLELNTGGRAGGEPIGLLKGFRCPGFGGVTGGMTAAATSAPRSAAAAPAGEADDAGEGTRGSEPPT
EAAFD*
MSHLNRFFKFLISRQTLVFVAIVAVAAAIWFIGPLIAFGDYRPLATVLARALSIVTLLAMLLFCLLGWSLGVIGAALLCL
AIWYLGPLFMFGSVRPLASTGVRVLLIAVLVACYAAYGLYRLWKAMRSNDALLQRILNPFSTQASDDAPAREQIRAVNSV
VTKAIDKVKQLRGGAFGLRRLLESQRYLYALPWYLVMGSPGAGKTTMILNSGLEFPDADQMVAASLGHHAETVNCDWWFG
NDAILIDTAGRYLMHDMPARADEPDHAAGAVGSANAAEWRGFLALLRKRRPRAPVNGVLLVVSVEELLTCSPSELKLLAA
AMRARLGELKHDLRIRYPAYLVVTKLDLLPGFLEYFQSLTAESRAQILGFTLPYHRDAPEERRDALRLACIEELRELEQG
FEDGLNARLQEEYELDRRRRLYALPAEFRSLCAILVEFVASVFVDSKYDDTELNGVLRGVYFSSAAQTHDVVPADRDTLL
QRLRRGLAARFGADAAAVSAPAPDGAAPVEYRGYFLRNLFQRVIVAEGHLVRPNLRWEIRFRALRVVGHLLSIAVAAWLV
YGMAGSFQRNRDYLAAIDHQTNALAARVQAQRRTPKPGDTGALLSASLDLPRFGELDLDRPGIAWRYGLYAAPGVVDASS
RTYAALLDQMLLPPVVARIATVLDGEVRAQNADEVYKTLPVYLMLYDPSHYDAKEVKAWVMRDWEQAGSVAMLGDRDVVA
GHLDALFRDGVPVTPATPMDGALIKRARELLGRNSASGRLYERAMKAMEPDAPENITLARAGGPQALSVFAMAGDPALAQ
GVPGLYTYEGYHEVFTRRLPEFLEQAHERDAWLMGRGDRSSTWGRAGRVLRAGERALADEIRRQYLIDYGNYWQRFLDGV
RLASGGAAPALDLQMLRTLSAPDSPLVRLARTAARETSLATPDTQDGPSLAGSAAAIASRRSRAASAVLAIGGATSTINR
LARRRMEHDLVEQRFAALRAVVTGEADTDGGKLVRAWSASASLSSLPSLPSPGGGGPMQLDGIVSLIGERYTRLVLAGDV
LATDSMPPADDLGATMRLEAEKLPAPFRAVLAGLAERSTQEVNRKMGALLGSRLEANVGDPCRSLIEGRYSFADSAQEVS
IADFNRVFAAKGLLDKFFRETLADHVDTGSHPWRYRPAAPGMPPMRGPSLEPFERAAAIRRAFFLGPGASRLVWKLGIRV
VSLDPDITELRLDIDGQSLRYAHGPVTTFPVTWPGPRGGSMAGITAEPQVSAQSSTLGAEGPWALFRLFDRGRLVPTSDT
SRYTEDFDFDGRHAVLELNTGGRAGGEPIGLLKGFRCPGFGGVTGGMTAAATSAPRSAAAAPAGEADDAGEGTRGSEPPT
EAAFD*
Nucleotide sequence: 4098 bp
>T6CP259609 NC_012721:163266-167363 [Burkholderia glumae BGR1] [TssM]
ATGTCCCATCTGAACCGCTTCTTCAAATTCCTCATCTCGCGTCAAACGCTCGTGTTCGTGGCGATCGTGGCGGTCGCCGC
GGCCATCTGGTTCATCGGCCCGCTTATCGCGTTCGGCGACTATCGTCCTCTCGCGACGGTGCTGGCGCGCGCGCTGTCGA
TCGTCACGCTGCTAGCGATGTTGCTGTTCTGTTTGCTCGGTTGGTCGCTCGGCGTGATCGGGGCCGCGCTGCTTTGCCTC
GCGATCTGGTATCTCGGGCCGCTGTTCATGTTCGGCTCCGTGCGGCCGCTGGCGTCGACGGGCGTGCGCGTGCTGCTGAT
CGCGGTGCTGGTGGCCTGTTACGCGGCCTACGGCCTGTACCGGCTCTGGAAGGCAATGCGCAGCAACGACGCGCTGCTGC
AGCGCATCCTGAACCCGTTCTCCACCCAGGCTAGTGACGACGCGCCGGCGCGCGAGCAAATCCGCGCTGTCAACTCGGTG
GTGACGAAGGCCATCGACAAGGTCAAGCAACTGCGCGGTGGGGCGTTCGGCCTGCGCCGGCTGCTCGAAAGCCAGCGCTA
TCTGTACGCGTTGCCATGGTATCTGGTGATGGGTTCGCCGGGCGCGGGCAAGACCACGATGATCCTGAATTCCGGCCTCG
AGTTTCCCGATGCCGATCAGATGGTGGCGGCCTCGCTCGGCCACCACGCCGAAACGGTGAATTGCGATTGGTGGTTTGGC
AACGACGCGATCCTGATCGACACGGCGGGCCGTTACCTGATGCATGACATGCCGGCACGAGCCGATGAGCCCGACCACGC
CGCCGGCGCGGTGGGATCGGCGAATGCCGCCGAATGGCGCGGCTTCCTCGCTCTGCTGCGCAAGCGCCGGCCCCGCGCGC
CGGTCAATGGCGTGCTGCTGGTGGTCTCGGTCGAGGAACTGCTGACGTGCTCGCCGTCCGAGCTCAAGCTGCTCGCCGCG
GCGATGCGCGCGCGGCTCGGCGAGCTGAAGCACGATCTGCGGATTCGCTACCCTGCCTATCTGGTGGTCACGAAGCTCGA
CCTGCTGCCGGGCTTTCTCGAGTATTTCCAGTCGTTGACGGCGGAGAGTCGCGCGCAGATTCTCGGGTTTACGCTGCCCT
ATCATCGCGACGCGCCCGAGGAGCGGCGCGACGCGTTGCGTCTGGCCTGTATCGAGGAGCTGCGTGAACTGGAACAAGGT
TTCGAAGACGGCCTCAACGCGCGGCTGCAGGAGGAATACGAGCTCGATCGGCGTCGAAGGCTTTATGCGTTGCCGGCGGA
ATTTCGCAGCCTATGCGCGATACTGGTCGAATTCGTCGCGTCCGTGTTCGTCGATTCGAAATACGACGATACCGAGCTGA
ACGGCGTGCTGCGCGGCGTATATTTCAGTAGTGCGGCGCAGACTCACGACGTGGTGCCTGCCGATCGCGACACGCTGCTG
CAACGTCTGCGGCGAGGGCTGGCGGCCCGCTTCGGCGCGGATGCCGCAGCGGTCTCCGCACCGGCGCCGGACGGCGCCGC
GCCCGTCGAGTATCGCGGCTATTTCTTGCGCAACCTGTTCCAACGCGTGATCGTTGCCGAAGGGCATCTGGTGCGCCCGA
ATCTGCGCTGGGAAATCCGCTTTCGCGCGTTGCGGGTGGTCGGCCATCTGCTGTCAATCGCGGTAGCGGCTTGGCTCGTG
TATGGGATGGCCGGCAGTTTCCAGCGCAATCGCGACTATCTCGCTGCGATCGACCACCAGACGAATGCGCTCGCCGCGCG
CGTGCAGGCACAGCGCCGGACGCCGAAGCCGGGTGACACGGGCGCACTGCTGTCGGCGTCGCTCGATTTGCCTCGGTTCG
GTGAGCTCGACCTTGATCGCCCCGGCATCGCGTGGCGCTACGGGCTGTACGCGGCCCCCGGCGTGGTCGATGCTTCGAGC
CGAACCTATGCGGCGCTGCTCGACCAGATGCTGCTGCCGCCCGTCGTTGCCCGTATTGCGACGGTGCTCGACGGCGAGGT
GCGCGCGCAGAACGCCGACGAGGTCTACAAAACCCTGCCGGTCTATCTCATGCTCTACGATCCTTCGCACTACGATGCGA
AGGAGGTCAAGGCCTGGGTGATGCGAGACTGGGAGCAGGCGGGCAGCGTCGCGATGCTGGGCGATCGCGATGTGGTGGCG
GGCCATCTCGACGCGCTGTTTCGCGACGGTGTGCCCGTGACGCCCGCCACGCCGATGGACGGCGCGCTGATCAAGCGTGC
GCGCGAGCTGCTAGGCCGCAATTCGGCCTCGGGGCGGCTCTACGAGCGCGCAATGAAGGCGATGGAGCCCGATGCACCCG
AGAACATCACGCTGGCCCGCGCGGGCGGCCCGCAGGCGCTGTCGGTGTTCGCGATGGCCGGCGACCCGGCCCTCGCGCAG
GGAGTGCCGGGGCTCTATACCTACGAGGGCTATCACGAGGTTTTTACGCGGCGCCTGCCCGAATTTCTCGAGCAGGCGCA
TGAACGGGACGCATGGCTCATGGGGCGGGGCGATCGATCGTCGACATGGGGGCGTGCGGGCCGTGTACTGCGTGCCGGCG
AGCGGGCGCTCGCCGACGAGATCCGCCGTCAGTACCTGATCGATTACGGGAATTACTGGCAGCGCTTTCTCGACGGCGTG
CGGCTCGCTTCCGGCGGCGCTGCACCGGCGCTCGACCTGCAGATGCTGCGGACGCTTTCCGCGCCCGATTCGCCGCTCGT
CAGACTGGCACGCACGGCCGCTCGCGAGACCTCGCTCGCGACGCCCGATACGCAGGACGGCCCATCGCTGGCCGGCAGCG
CCGCGGCCATCGCGTCGCGGCGCTCGCGCGCCGCCTCGGCCGTGCTCGCGATCGGCGGCGCCACCTCGACGATCAATCGG
CTGGCGCGACGCAGGATGGAGCACGATCTGGTCGAGCAGCGCTTCGCCGCCTTGCGTGCCGTCGTCACGGGCGAGGCCGA
CACCGATGGCGGCAAGTTGGTGCGGGCTTGGTCGGCATCGGCCTCGTTGTCGTCGCTGCCGTCGCTACCTTCGCCGGGAG
GCGGCGGGCCGATGCAGCTCGACGGCATCGTCAGCTTGATCGGCGAGCGCTATACACGCCTCGTGCTGGCCGGTGACGTG
CTCGCCACTGATTCCATGCCACCCGCCGACGATCTGGGTGCCACGATGCGGCTCGAGGCCGAAAAGTTGCCGGCACCGTT
TCGCGCTGTACTGGCTGGTTTGGCCGAGCGCTCCACCCAGGAGGTCAATCGCAAGATGGGCGCGCTGCTCGGTTCGCGGC
TGGAGGCCAACGTGGGCGATCCGTGTCGCAGCCTGATCGAAGGCCGGTATTCGTTCGCGGACAGCGCGCAGGAGGTCAGC
ATCGCCGATTTCAACCGCGTGTTCGCCGCGAAGGGCCTGCTCGACAAGTTCTTTCGCGAGACCCTTGCCGACCACGTCGA
TACGGGTAGCCACCCATGGCGCTATCGGCCGGCCGCTCCCGGGATGCCACCGATGCGCGGGCCGAGCCTCGAGCCGTTCG
AGCGCGCCGCCGCGATCCGCAGGGCGTTCTTCCTCGGTCCGGGCGCGAGCCGGCTGGTCTGGAAACTTGGCATCCGGGTG
GTGTCGCTCGATCCCGATATTACTGAACTGCGTCTCGATATCGACGGGCAGAGCCTGCGTTACGCGCATGGCCCGGTCAC
CACCTTCCCGGTCACCTGGCCGGGACCGCGCGGTGGCTCGATGGCTGGCATCACGGCAGAGCCGCAGGTTAGCGCGCAGA
GTTCGACGCTTGGCGCGGAGGGGCCGTGGGCGCTCTTCCGCCTGTTCGATCGCGGCCGTCTGGTGCCGACATCGGACACG
TCGCGCTACACCGAGGATTTCGATTTCGACGGCCGGCACGCGGTGCTCGAACTGAACACTGGCGGCCGGGCCGGAGGGGA
GCCGATTGGATTGTTGAAGGGGTTTCGCTGCCCGGGCTTCGGTGGCGTGACCGGCGGGATGACAGCGGCCGCCACCTCGG
CTCCGCGGTCCGCTGCGGCGGCACCGGCGGGCGAAGCGGATGACGCTGGAGAGGGCACACGCGGCTCGGAGCCGCCCACC
GAGGCGGCGTTCGATTAA
ATGTCCCATCTGAACCGCTTCTTCAAATTCCTCATCTCGCGTCAAACGCTCGTGTTCGTGGCGATCGTGGCGGTCGCCGC
GGCCATCTGGTTCATCGGCCCGCTTATCGCGTTCGGCGACTATCGTCCTCTCGCGACGGTGCTGGCGCGCGCGCTGTCGA
TCGTCACGCTGCTAGCGATGTTGCTGTTCTGTTTGCTCGGTTGGTCGCTCGGCGTGATCGGGGCCGCGCTGCTTTGCCTC
GCGATCTGGTATCTCGGGCCGCTGTTCATGTTCGGCTCCGTGCGGCCGCTGGCGTCGACGGGCGTGCGCGTGCTGCTGAT
CGCGGTGCTGGTGGCCTGTTACGCGGCCTACGGCCTGTACCGGCTCTGGAAGGCAATGCGCAGCAACGACGCGCTGCTGC
AGCGCATCCTGAACCCGTTCTCCACCCAGGCTAGTGACGACGCGCCGGCGCGCGAGCAAATCCGCGCTGTCAACTCGGTG
GTGACGAAGGCCATCGACAAGGTCAAGCAACTGCGCGGTGGGGCGTTCGGCCTGCGCCGGCTGCTCGAAAGCCAGCGCTA
TCTGTACGCGTTGCCATGGTATCTGGTGATGGGTTCGCCGGGCGCGGGCAAGACCACGATGATCCTGAATTCCGGCCTCG
AGTTTCCCGATGCCGATCAGATGGTGGCGGCCTCGCTCGGCCACCACGCCGAAACGGTGAATTGCGATTGGTGGTTTGGC
AACGACGCGATCCTGATCGACACGGCGGGCCGTTACCTGATGCATGACATGCCGGCACGAGCCGATGAGCCCGACCACGC
CGCCGGCGCGGTGGGATCGGCGAATGCCGCCGAATGGCGCGGCTTCCTCGCTCTGCTGCGCAAGCGCCGGCCCCGCGCGC
CGGTCAATGGCGTGCTGCTGGTGGTCTCGGTCGAGGAACTGCTGACGTGCTCGCCGTCCGAGCTCAAGCTGCTCGCCGCG
GCGATGCGCGCGCGGCTCGGCGAGCTGAAGCACGATCTGCGGATTCGCTACCCTGCCTATCTGGTGGTCACGAAGCTCGA
CCTGCTGCCGGGCTTTCTCGAGTATTTCCAGTCGTTGACGGCGGAGAGTCGCGCGCAGATTCTCGGGTTTACGCTGCCCT
ATCATCGCGACGCGCCCGAGGAGCGGCGCGACGCGTTGCGTCTGGCCTGTATCGAGGAGCTGCGTGAACTGGAACAAGGT
TTCGAAGACGGCCTCAACGCGCGGCTGCAGGAGGAATACGAGCTCGATCGGCGTCGAAGGCTTTATGCGTTGCCGGCGGA
ATTTCGCAGCCTATGCGCGATACTGGTCGAATTCGTCGCGTCCGTGTTCGTCGATTCGAAATACGACGATACCGAGCTGA
ACGGCGTGCTGCGCGGCGTATATTTCAGTAGTGCGGCGCAGACTCACGACGTGGTGCCTGCCGATCGCGACACGCTGCTG
CAACGTCTGCGGCGAGGGCTGGCGGCCCGCTTCGGCGCGGATGCCGCAGCGGTCTCCGCACCGGCGCCGGACGGCGCCGC
GCCCGTCGAGTATCGCGGCTATTTCTTGCGCAACCTGTTCCAACGCGTGATCGTTGCCGAAGGGCATCTGGTGCGCCCGA
ATCTGCGCTGGGAAATCCGCTTTCGCGCGTTGCGGGTGGTCGGCCATCTGCTGTCAATCGCGGTAGCGGCTTGGCTCGTG
TATGGGATGGCCGGCAGTTTCCAGCGCAATCGCGACTATCTCGCTGCGATCGACCACCAGACGAATGCGCTCGCCGCGCG
CGTGCAGGCACAGCGCCGGACGCCGAAGCCGGGTGACACGGGCGCACTGCTGTCGGCGTCGCTCGATTTGCCTCGGTTCG
GTGAGCTCGACCTTGATCGCCCCGGCATCGCGTGGCGCTACGGGCTGTACGCGGCCCCCGGCGTGGTCGATGCTTCGAGC
CGAACCTATGCGGCGCTGCTCGACCAGATGCTGCTGCCGCCCGTCGTTGCCCGTATTGCGACGGTGCTCGACGGCGAGGT
GCGCGCGCAGAACGCCGACGAGGTCTACAAAACCCTGCCGGTCTATCTCATGCTCTACGATCCTTCGCACTACGATGCGA
AGGAGGTCAAGGCCTGGGTGATGCGAGACTGGGAGCAGGCGGGCAGCGTCGCGATGCTGGGCGATCGCGATGTGGTGGCG
GGCCATCTCGACGCGCTGTTTCGCGACGGTGTGCCCGTGACGCCCGCCACGCCGATGGACGGCGCGCTGATCAAGCGTGC
GCGCGAGCTGCTAGGCCGCAATTCGGCCTCGGGGCGGCTCTACGAGCGCGCAATGAAGGCGATGGAGCCCGATGCACCCG
AGAACATCACGCTGGCCCGCGCGGGCGGCCCGCAGGCGCTGTCGGTGTTCGCGATGGCCGGCGACCCGGCCCTCGCGCAG
GGAGTGCCGGGGCTCTATACCTACGAGGGCTATCACGAGGTTTTTACGCGGCGCCTGCCCGAATTTCTCGAGCAGGCGCA
TGAACGGGACGCATGGCTCATGGGGCGGGGCGATCGATCGTCGACATGGGGGCGTGCGGGCCGTGTACTGCGTGCCGGCG
AGCGGGCGCTCGCCGACGAGATCCGCCGTCAGTACCTGATCGATTACGGGAATTACTGGCAGCGCTTTCTCGACGGCGTG
CGGCTCGCTTCCGGCGGCGCTGCACCGGCGCTCGACCTGCAGATGCTGCGGACGCTTTCCGCGCCCGATTCGCCGCTCGT
CAGACTGGCACGCACGGCCGCTCGCGAGACCTCGCTCGCGACGCCCGATACGCAGGACGGCCCATCGCTGGCCGGCAGCG
CCGCGGCCATCGCGTCGCGGCGCTCGCGCGCCGCCTCGGCCGTGCTCGCGATCGGCGGCGCCACCTCGACGATCAATCGG
CTGGCGCGACGCAGGATGGAGCACGATCTGGTCGAGCAGCGCTTCGCCGCCTTGCGTGCCGTCGTCACGGGCGAGGCCGA
CACCGATGGCGGCAAGTTGGTGCGGGCTTGGTCGGCATCGGCCTCGTTGTCGTCGCTGCCGTCGCTACCTTCGCCGGGAG
GCGGCGGGCCGATGCAGCTCGACGGCATCGTCAGCTTGATCGGCGAGCGCTATACACGCCTCGTGCTGGCCGGTGACGTG
CTCGCCACTGATTCCATGCCACCCGCCGACGATCTGGGTGCCACGATGCGGCTCGAGGCCGAAAAGTTGCCGGCACCGTT
TCGCGCTGTACTGGCTGGTTTGGCCGAGCGCTCCACCCAGGAGGTCAATCGCAAGATGGGCGCGCTGCTCGGTTCGCGGC
TGGAGGCCAACGTGGGCGATCCGTGTCGCAGCCTGATCGAAGGCCGGTATTCGTTCGCGGACAGCGCGCAGGAGGTCAGC
ATCGCCGATTTCAACCGCGTGTTCGCCGCGAAGGGCCTGCTCGACAAGTTCTTTCGCGAGACCCTTGCCGACCACGTCGA
TACGGGTAGCCACCCATGGCGCTATCGGCCGGCCGCTCCCGGGATGCCACCGATGCGCGGGCCGAGCCTCGAGCCGTTCG
AGCGCGCCGCCGCGATCCGCAGGGCGTTCTTCCTCGGTCCGGGCGCGAGCCGGCTGGTCTGGAAACTTGGCATCCGGGTG
GTGTCGCTCGATCCCGATATTACTGAACTGCGTCTCGATATCGACGGGCAGAGCCTGCGTTACGCGCATGGCCCGGTCAC
CACCTTCCCGGTCACCTGGCCGGGACCGCGCGGTGGCTCGATGGCTGGCATCACGGCAGAGCCGCAGGTTAGCGCGCAGA
GTTCGACGCTTGGCGCGGAGGGGCCGTGGGCGCTCTTCCGCCTGTTCGATCGCGGCCGTCTGGTGCCGACATCGGACACG
TCGCGCTACACCGAGGATTTCGATTTCGACGGCCGGCACGCGGTGCTCGAACTGAACACTGGCGGCCGGGCCGGAGGGGA
GCCGATTGGATTGTTGAAGGGGTTTCGCTGCCCGGGCTTCGGTGGCGTGACCGGCGGGATGACAGCGGCCGCCACCTCGG
CTCCGCGGTCCGCTGCGGCGGCACCGGCGGGCGAAGCGGATGACGCTGGAGAGGGCACACGCGGCTCGGAGCCGCCCACC
GAGGCGGCGTTCGATTAA