Detailed information of component protein


Summary

Component ID T6CP259578
Component protein type TssM
T6SS ID (Type) T6SS17506 experimental (Type i1)
Strain Aliivibrio fischeri strain ES401
Replicon chromosome
Sequence Protein sequence (1133 a.a.); Nucleotide sequence (3399 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) VFES401_RS15765
Coordinate (Strand) 370428..373826 (+)
NCBI ID WP_063658813.1
RefSeq NZ_SRJG01000005
Uniprot ID UPI0007C44595
KEGG ID -
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related_N PF14331.8 1.8e-43 155..408
IcmF-related PF06761.14 1.7e-41 486..756
IcmF_C PF06744.14 3e-15 1004..1106

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-10 MIKNTLARTP
Transmembrane helix 11-33 FILSTVISAFFALLLVGVYYLWF
Outside 34-37 NQAD
Transmembrane helix 38-60 LRLLWGMLGVIFSIYAIFNLTLY
Inside 61-401 IKKRKSKSHQNIETEEDTLSMVLKPLLNKAGKKPVYLVLGNKGSGKNQFLNVSNAIKPMDKVKNVKNDFFEWSESDSAVYIKPNHRLTFQEISSNDSLLWDALINEVIKHKPRKPFAGCMLLIDFEFLIVHDDEQTDYTLNALLQRLESIKETTGATLPLYLIMTKLDKLEGFKEYIHFSPLKSSVEFLAIPLKDAKGAILDYFRDSYKNIVKVMESNALDSSSYTNNPDEKQTILAFPKQFELCEKEISRIIERLNELNQGTYSVDIREVFFSSSLQGGRKYNLLAKSCSNYFNLPIIASEHGQLTETPYFTRFLVDAKVLPESDYSAENQHYLKRIQQH
Transmembrane helix 402-424 SRIAFTASAILLTSGGYFLFQAL
Outside 425-1132 DSNLRVINQLIQVEDTDLADYHSKDFTAKLINAQKAISPTYSAWLSGNRALDEEMLTMNVSRLEPTTKIAYNALIAQIDEFLMPVISEGYENLLTKNQNSVSTSLPLLKGYLMLNDPSKRDIPYLKQQTFDVLSTLSNDSVTVENTMNYLDAYFRTQFAPIDINMDLVRATRRSLLAKSNVDLVYAQILDQAKSIDLDTLDIQRAVGFDFTNIFEKPTTESNLQISKVYTASGFSSFYRPHIDLLSKRVIADNWVLGLSQHIIPTDDEQKDFKSDVRKKYTDDYINYWRNALSELKIKNFSNIYDLNNAIDLISGPSSPMTTVLKQLYSNTQFSSTADITSLMDAKKDLKGALETATDAVKEIIEPDYVLMSRVEQAFTLLNQLQISETKNSPTPWDDIVASLNQVRTYVKDIADSPNVHQAALDAARARMNRAEADPLVRLKQIAQKSPEPVRSWLLDIVNQTWSVMIVEATKGIQTRWYSEVYSIFKATGLNRYPFNTSATEEISLEDFEQLFATGGVLDVFIKDNLAPFYDINLWTAKRVDGQIMYLSPQFLVQLKNYNIIRNTMINKSTNKVFIPFNAKVIDLDSSAIRANINFGSTIMSYYHGPSKVQELQWPPKGGDFEVKFTLQDVTDEGKQHTLSKNGQWAIYRLLGESTLSDITNTNFVSHINVSGRDISMLFTPLTQNNPFTFAELANFKLPEVINKK



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9936 - -



  Protein sequence: 1133 a.a.    

>T6CP259578 NZ_SRJG01000005:370428-373826 [Aliivibrio fischeri] [TssM]
MIKNTLARTPFILSTVISAFFALLLVGVYYLWFNQADLRLLWGMLGVIFSIYAIFNLTLYIKKRKSKSHQNIETEEDTLS
MVLKPLLNKAGKKPVYLVLGNKGSGKNQFLNVSNAIKPMDKVKNVKNDFFEWSESDSAVYIKPNHRLTFQEISSNDSLLW
DALINEVIKHKPRKPFAGCMLLIDFEFLIVHDDEQTDYTLNALLQRLESIKETTGATLPLYLIMTKLDKLEGFKEYIHFS
PLKSSVEFLAIPLKDAKGAILDYFRDSYKNIVKVMESNALDSSSYTNNPDEKQTILAFPKQFELCEKEISRIIERLNELN
QGTYSVDIREVFFSSSLQGGRKYNLLAKSCSNYFNLPIIASEHGQLTETPYFTRFLVDAKVLPESDYSAENQHYLKRIQQ
HSRIAFTASAILLTSGGYFLFQALDSNLRVINQLIQVEDTDLADYHSKDFTAKLINAQKAISPTYSAWLSGNRALDEEML
TMNVSRLEPTTKIAYNALIAQIDEFLMPVISEGYENLLTKNQNSVSTSLPLLKGYLMLNDPSKRDIPYLKQQTFDVLSTL
SNDSVTVENTMNYLDAYFRTQFAPIDINMDLVRATRRSLLAKSNVDLVYAQILDQAKSIDLDTLDIQRAVGFDFTNIFEK
PTTESNLQISKVYTASGFSSFYRPHIDLLSKRVIADNWVLGLSQHIIPTDDEQKDFKSDVRKKYTDDYINYWRNALSELK
IKNFSNIYDLNNAIDLISGPSSPMTTVLKQLYSNTQFSSTADITSLMDAKKDLKGALETATDAVKEIIEPDYVLMSRVEQ
AFTLLNQLQISETKNSPTPWDDIVASLNQVRTYVKDIADSPNVHQAALDAARARMNRAEADPLVRLKQIAQKSPEPVRSW
LLDIVNQTWSVMIVEATKGIQTRWYSEVYSIFKATGLNRYPFNTSATEEISLEDFEQLFATGGVLDVFIKDNLAPFYDIN
LWTAKRVDGQIMYLSPQFLVQLKNYNIIRNTMINKSTNKVFIPFNAKVIDLDSSAIRANINFGSTIMSYYHGPSKVQELQ
WPPKGGDFEVKFTLQDVTDEGKQHTLSKNGQWAIYRLLGESTLSDITNTNFVSHINVSGRDISMLFTPLTQNNPFTFAEL
ANFKLPEVINKK*

  Nucleotide sequence: 3399 bp    

>T6CP259578 NZ_SRJG01000005:370428-373826 [Aliivibrio fischeri] [TssM]
ATGATTAAAAATACTTTGGCTAGAACACCTTTCATTTTATCGACAGTAATTTCAGCATTTTTTGCATTACTTTTAGTTGG
TGTATATTACTTATGGTTCAATCAAGCAGACCTACGTTTATTATGGGGGATGCTTGGTGTCATTTTTTCCATCTACGCTA
TTTTTAACCTGACTCTTTACATAAAAAAACGTAAAAGCAAAAGCCATCAAAATATCGAAACGGAAGAAGATACACTATCT
ATGGTTTTAAAACCTCTGCTTAATAAAGCGGGAAAGAAACCAGTGTATCTTGTTCTTGGTAATAAAGGGTCAGGTAAAAA
TCAATTCTTAAATGTTTCAAATGCCATTAAACCAATGGATAAAGTGAAAAACGTTAAAAATGATTTCTTTGAATGGTCAG
AATCCGATTCAGCTGTATACATCAAGCCTAACCATCGACTAACTTTTCAAGAAATATCGAGTAATGATAGTTTACTGTGG
GATGCTTTAATTAATGAAGTGATCAAACATAAACCTCGTAAGCCTTTTGCGGGCTGTATGTTATTAATTGATTTTGAATT
TTTAATCGTACATGACGATGAACAAACTGATTACACATTAAATGCCCTACTCCAAAGACTGGAATCTATAAAAGAAACGA
CAGGTGCAACATTACCTCTGTATCTTATTATGACTAAGTTAGATAAATTGGAAGGTTTTAAAGAATACATCCACTTCAGC
CCTCTTAAATCGAGCGTTGAGTTTTTAGCTATTCCACTAAAAGACGCGAAAGGTGCCATTTTAGATTATTTCCGTGACAG
CTATAAAAACATAGTGAAAGTAATGGAATCTAATGCCCTTGACTCATCGTCGTACACTAACAATCCTGATGAAAAACAGA
CAATTTTAGCTTTTCCTAAACAATTTGAACTATGTGAGAAAGAAATATCTCGCATTATCGAGCGTTTGAATGAATTAAAT
CAAGGTACCTACTCCGTTGATATTCGTGAAGTGTTTTTTAGCTCTAGTTTACAAGGTGGCCGAAAATATAACCTATTAGC
TAAAAGTTGTAGCAACTATTTCAATTTACCGATTATTGCATCTGAGCATGGCCAATTAACTGAAACGCCTTATTTTACCC
GCTTCTTAGTAGACGCAAAGGTATTACCTGAGTCTGATTATTCTGCTGAAAACCAGCATTACCTAAAGCGTATTCAACAA
CATAGCCGTATCGCTTTTACTGCTTCAGCTATATTGCTTACTAGTGGCGGATATTTCTTATTCCAAGCACTTGATAGTAA
CTTACGAGTTATTAACCAATTAATTCAAGTTGAAGATACAGATCTTGCTGATTACCACAGTAAAGATTTTACTGCCAAAT
TAATCAATGCTCAAAAGGCCATTTCACCAACTTATTCAGCATGGTTAAGCGGTAATCGCGCTTTAGATGAAGAAATGTTG
ACCATGAACGTTAGCCGTTTAGAGCCTACAACAAAAATTGCATACAATGCCCTAATTGCGCAAATAGATGAATTTTTAAT
GCCTGTTATTTCTGAAGGTTATGAGAACTTATTAACTAAAAATCAAAATTCAGTATCTACGTCATTGCCCTTGTTAAAAG
GATATTTGATGCTGAATGATCCAAGCAAACGTGATATTCCCTATTTAAAGCAACAGACCTTTGATGTGTTATCAACACTA
AGTAATGACTCTGTTACAGTTGAAAATACCATGAATTACCTTGATGCGTACTTCAGAACTCAATTTGCTCCTATTGATAT
AAATATGGATCTCGTTCGTGCTACCCGTCGTAGTCTATTGGCAAAATCAAATGTTGATTTAGTCTATGCTCAAATTTTAG
ATCAAGCGAAAAGCATCGATTTAGATACGTTAGATATTCAAAGAGCCGTTGGTTTTGATTTTACTAATATATTTGAAAAA
CCAACTACCGAAAGTAACTTACAAATCAGCAAGGTATATACTGCATCTGGTTTTAGTTCATTCTATCGTCCGCACATTGA
TCTTTTGTCAAAACGTGTTATCGCGGATAACTGGGTACTTGGTTTATCTCAACATATAATCCCTACTGATGATGAGCAAA
AAGATTTTAAAAGTGATGTGCGTAAAAAATACACTGATGACTACATCAACTATTGGCGTAATGCACTAAGTGAATTAAAG
ATTAAAAACTTTTCAAACATTTACGACTTGAATAATGCAATCGACCTAATTTCTGGTCCATCATCTCCGATGACAACCGT
ATTAAAACAGCTGTATTCGAATACACAGTTTTCTTCAACGGCTGACATCACTTCTTTGATGGATGCAAAAAAAGATCTAA
AGGGTGCTTTAGAAACAGCTACAGATGCAGTTAAAGAGATTATAGAACCTGATTACGTTTTAATGTCTCGTGTAGAGCAA
GCCTTTACTTTGCTTAACCAGCTACAAATTAGTGAAACAAAAAACTCTCCTACACCATGGGATGATATTGTTGCTTCATT
AAACCAAGTAAGAACGTATGTAAAAGACATTGCTGATTCCCCCAATGTCCATCAAGCGGCACTCGACGCTGCTAGAGCTC
GAATGAATAGAGCAGAAGCAGATCCATTAGTGCGCTTGAAACAAATTGCACAGAAATCGCCAGAGCCCGTAAGAAGCTGG
CTATTAGATATAGTGAATCAAACATGGTCAGTCATGATTGTTGAAGCCACTAAAGGTATCCAAACACGTTGGTATAGTGA
AGTTTATAGTATTTTTAAGGCAACGGGGTTAAATCGTTACCCATTCAATACCTCAGCTACCGAAGAAATCTCTCTTGAAG
ATTTTGAACAATTATTTGCGACAGGTGGAGTACTCGATGTCTTTATTAAGGATAACTTAGCACCATTTTACGACATTAAT
TTATGGACCGCTAAACGCGTTGATGGACAGATAATGTACTTATCCCCTCAATTTTTAGTTCAATTAAAAAACTACAATAT
TATTCGTAACACGATGATCAACAAAAGCACAAATAAAGTATTTATTCCTTTTAATGCGAAGGTCATCGACTTAGATTCAA
GTGCCATTAGAGCTAATATTAACTTTGGCAGCACAATAATGAGCTACTACCATGGGCCAAGTAAGGTACAAGAACTTCAA
TGGCCACCAAAAGGCGGCGATTTTGAAGTTAAATTTACCCTACAAGACGTAACTGATGAAGGTAAGCAACACACCTTATC
TAAAAATGGTCAATGGGCTATATATCGCCTACTAGGCGAATCAACCTTATCTGACATTACAAATACAAATTTTGTAAGTC
ATATAAATGTGTCAGGACGTGACATCAGCATGCTATTTACGCCTTTGACACAAAATAACCCTTTTACTTTCGCTGAGCTT
GCAAACTTTAAGCTACCAGAAGTAATCAACAAAAAATAA