Detailed information of component protein
Summary
| Component ID | T6CP259576 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS17506  (Type i1) |
| Strain | Aliivibrio fischeri strain ES401 |
| Replicon | chromosome |
| Sequence | Protein sequence (230 a.a.); Nucleotide sequence (690 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VFES401_RS15720 |
| Coordinate (Strand) | 352287..352976 (+) |
| NCBI ID | WP_081251043.1 |
| RefSeq | NZ_SRJG01000005 |
| Uniprot ID | UPI0009C17BE4 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 5.5e-48 | 26..213 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-196 | MRTINFIEIKLTSLKKKIINAQLSLSCMSSIPWLDDPMPFREQVAREIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGTNSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPK |
| Transmembrane helix | 197-219 | LILGVSALLFAAMYAATYLVIDV |
| Outside | 220-229 | RFQKLLSLYQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.5352 | - | - |
Protein sequence: 230 a.a.
>T6CP259576 NZ_SRJG01000005:352287-352976 [Aliivibrio fischeri] [TssL]
MRTINFIEIKLTSLKKKIINAQLSLSCMSSIPWLDDPMPFREQVAREIRKGERMLNEMEVDRASILVIRYCLCAAMDESV
CRQEWGTNSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTI
YRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPKLILGVSALLFAAMYAATYLVIDVRFQKLLSLYQ*
MRTINFIEIKLTSLKKKIINAQLSLSCMSSIPWLDDPMPFREQVAREIRKGERMLNEMEVDRASILVIRYCLCAAMDESV
CRQEWGTNSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTI
YRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPKLILGVSALLFAAMYAATYLVIDVRFQKLLSLYQ*
Nucleotide sequence: 690 bp
>T6CP259576 NZ_SRJG01000005:352287-352976 [Aliivibrio fischeri] [TssL]
CTGCGTACTATAAACTTTATTGAAATTAAACTAACTTCGCTTAAGAAAAAAATAATTAATGCACAGCTCTCTTTATCTTG
TATGTCTTCCATTCCATGGCTTGATGATCCTATGCCTTTTCGTGAGCAAGTCGCTCGTGAAATACGTAAAGGTGAACGTA
TGCTCAACGAAATGGAAGTCGACCGTGCATCTATTCTGGTTATTCGTTACTGCTTATGTGCTGCAATGGATGAATCGGTA
TGCCGTCAAGAATGGGGAACCAACAGTAACTGGAGCCAGAACAGCTTATTGTCTGAATTTCATAATGAAACCTCAGGTGG
CGATAAGTTTTTTGTCATTTTAGATAGATTAAAAGCAGATCCTCGCAAGTATCGTCATGTAATCGAATTCTTATATTTAC
TCCTACAACTTGGTTTTAAAGGTAAATATGGTCGAGCTGAGCGTGGTGCAGAGAGCTTAACTGAGATTGGCAACACTATC
TACCGTTTAGTTCGTGACGAGCGTCTAGCTGAGCAAGAGAAAGTAGCATTAGTTGATGTCAAATCTAAGTACTTAAAAAA
ACCATTAAAACGCGTACTGCCTCCCAAGTTGATCCTTGGCGTCAGTGCTTTACTATTTGCGGCCATGTATGCCGCTACGT
ATCTCGTTATTGATGTTCGATTTCAAAAGTTACTATCTCTTTATCAGTAG
CTGCGTACTATAAACTTTATTGAAATTAAACTAACTTCGCTTAAGAAAAAAATAATTAATGCACAGCTCTCTTTATCTTG
TATGTCTTCCATTCCATGGCTTGATGATCCTATGCCTTTTCGTGAGCAAGTCGCTCGTGAAATACGTAAAGGTGAACGTA
TGCTCAACGAAATGGAAGTCGACCGTGCATCTATTCTGGTTATTCGTTACTGCTTATGTGCTGCAATGGATGAATCGGTA
TGCCGTCAAGAATGGGGAACCAACAGTAACTGGAGCCAGAACAGCTTATTGTCTGAATTTCATAATGAAACCTCAGGTGG
CGATAAGTTTTTTGTCATTTTAGATAGATTAAAAGCAGATCCTCGCAAGTATCGTCATGTAATCGAATTCTTATATTTAC
TCCTACAACTTGGTTTTAAAGGTAAATATGGTCGAGCTGAGCGTGGTGCAGAGAGCTTAACTGAGATTGGCAACACTATC
TACCGTTTAGTTCGTGACGAGCGTCTAGCTGAGCAAGAGAAAGTAGCATTAGTTGATGTCAAATCTAAGTACTTAAAAAA
ACCATTAAAACGCGTACTGCCTCCCAAGTTGATCCTTGGCGTCAGTGCTTTACTATTTGCGGCCATGTATGCCGCTACGT
ATCTCGTTATTGATGTTCGATTTCAAAAGTTACTATCTCTTTATCAGTAG