Detailed information of component protein
Summary
| Component ID | T6CP259575 |
| Component protein type | TssL |
| T6SS ID (Type) | T6SS17506  (Type i1) |
| Strain | Aliivibrio fischeri strain ES401 |
| Replicon | chromosome |
| Sequence | Protein sequence (264 a.a.); Nucleotide sequence (792 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VFES401_RS15700 |
| Coordinate (Strand) | 346893..347684 (+) |
| NCBI ID | WP_012534924.1 |
| RefSeq | NZ_SRJG01000005 |
| Uniprot ID | B5EUI9_ALIFM, A0A1B9P6R2_ALIFS |
| KEGG ID | vfm:VFMJ11_A0808 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 1.8e-49 | 47..247 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-230 | MSYVEDDVTVVLFQPEPGKPMEVMPAPDLSRNISVSELDISSMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAREIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGANSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRHVIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPK |
| Transmembrane helix | 231-253 | LILGVSALLFAAMYAATYLVIDV |
| Outside | 254-263 | RFQKLLSLYQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9938 | - | - |
Protein sequence: 264 a.a.
>T6CP259575 NZ_SRJG01000005:346893-347684 [Aliivibrio fischeri] [TssL]
MSYVEDDVTVVLFQPEPGKPMEVMPAPDLSRNISVSELDISSMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGANSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRH
VIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPKLILGVSALLF
AAMYAATYLVIDVRFQKLLSLYQ*
MSYVEDDVTVVLFQPEPGKPMEVMPAPDLSRNISVSELDISSMGINPLVDQFSWLIASLSCMSSIPWLDDPMPFREQVAR
EIRKGERMLNEMEVDRASILVIRYCLCAAMDESVCRQEWGANSNWSQNSLLSEFHNETSGGDKFFVILDRLKADPRKYRH
VIEFLYLLLQLGFKGKYGRAERGAESLTEIGNTIYRLVRDERLAEQEKVALVDVKSKYLKKPLKRVLPPKLILGVSALLF
AAMYAATYLVIDVRFQKLLSLYQ*
Nucleotide sequence: 792 bp
>T6CP259575 NZ_SRJG01000005:346893-347684 [Aliivibrio fischeri] [TssL]
ATGAGTTATGTTGAAGACGACGTAACAGTTGTACTTTTTCAACCTGAGCCGGGCAAACCCATGGAGGTTATGCCCGCTCC
TGATTTATCTCGCAATATTTCAGTCAGTGAATTGGATATTTCATCTATGGGGATCAACCCATTAGTTGACCAATTCTCAT
GGCTTATTGCGAGTTTATCTTGTATGTCTTCCATTCCATGGCTTGATGATCCTATGCCTTTTCGTGAGCAAGTCGCTCGT
GAAATACGTAAAGGTGAACGTATGCTCAACGAAATGGAAGTCGACCGTGCATCTATTCTGGTTATCCGTTATTGCTTATG
TGCTGCAATGGATGAATCGGTATGCCGTCAAGAATGGGGAGCCAACAGTAATTGGAGCCAGAACAGCTTATTGTCTGAAT
TTCATAATGAAACCTCAGGTGGTGATAAGTTTTTTGTCATTTTAGATAGATTAAAAGCAGATCCTCGCAAGTATCGTCAT
GTGATTGAATTCTTATATTTACTCCTACAACTTGGTTTTAAAGGTAAATATGGTCGAGCTGAGCGTGGTGCCGAAAGTTT
GACAGAGATCGGCAACACTATCTACCGTTTGGTTCGTGACGAGCGTCTTGCTGAACAAGAGAAAGTAGCATTAGTTGATG
TTAAATCTAAGTACTTAAAAAAACCATTAAAACGCGTACTGCCTCCAAAGTTGATCCTTGGCGTCAGTGCTTTACTATTT
GCGGCCATGTATGCCGCTACGTATCTCGTTATTGATGTTCGATTTCAAAAGTTACTGTCTCTTTATCAGTAG
ATGAGTTATGTTGAAGACGACGTAACAGTTGTACTTTTTCAACCTGAGCCGGGCAAACCCATGGAGGTTATGCCCGCTCC
TGATTTATCTCGCAATATTTCAGTCAGTGAATTGGATATTTCATCTATGGGGATCAACCCATTAGTTGACCAATTCTCAT
GGCTTATTGCGAGTTTATCTTGTATGTCTTCCATTCCATGGCTTGATGATCCTATGCCTTTTCGTGAGCAAGTCGCTCGT
GAAATACGTAAAGGTGAACGTATGCTCAACGAAATGGAAGTCGACCGTGCATCTATTCTGGTTATCCGTTATTGCTTATG
TGCTGCAATGGATGAATCGGTATGCCGTCAAGAATGGGGAGCCAACAGTAATTGGAGCCAGAACAGCTTATTGTCTGAAT
TTCATAATGAAACCTCAGGTGGTGATAAGTTTTTTGTCATTTTAGATAGATTAAAAGCAGATCCTCGCAAGTATCGTCAT
GTGATTGAATTCTTATATTTACTCCTACAACTTGGTTTTAAAGGTAAATATGGTCGAGCTGAGCGTGGTGCCGAAAGTTT
GACAGAGATCGGCAACACTATCTACCGTTTGGTTCGTGACGAGCGTCTTGCTGAACAAGAGAAAGTAGCATTAGTTGATG
TTAAATCTAAGTACTTAAAAAAACCATTAAAACGCGTACTGCCTCCAAAGTTGATCCTTGGCGTCAGTGCTTTACTATTT
GCGGCCATGTATGCCGCTACGTATCTCGTTATTGATGTTCGATTTCAAAAGTTACTGTCTCTTTATCAGTAG