Detailed information of component protein
Summary
| Component ID | T6CP259573 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS17506  (Type i1) |
| Strain | Aliivibrio fischeri strain ES401 |
| Replicon | chromosome |
| Sequence | Protein sequence (167 a.a.); Nucleotide sequence (501 bp) |
| Reference |
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | VFES401_RS15690 |
| Coordinate (Strand) | 345059..345559 (+) |
| NCBI ID | WP_012535132.1 |
| RefSeq | NZ_SRJG01000005 |
| Uniprot ID | B5EUI7_ALIFM, A0A510UBV8_ALIFS |
| KEGG ID | vfm:VFMJ11_A0806 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 4e-29 | 34..150 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-166 | MRKLLTFSLIFLLSGCTMWDQFKESTGITPETSSIELIITADKVLNVREGGQSSPVILRIHELNSPVLFRNLDFFALFENDKDALGDEYIKRYEYQIQPGDVVHETLVLDPATRAIGFSVAFRDINGSSWRKVELIEEKSEYFLKLNLKESELSSNNTRGIEQIYF |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9984 | pos: 15-16. LSG-CT. | MRKLLTFSLIFLLSG |
Protein sequence: 167 a.a.
>T6CP259573 NZ_SRJG01000005:345059-345559 [Aliivibrio fischeri] [TssJ]
MRKLLTFSLIFLLSGCTMWDQFKESTGITPETSSIELIITADKVLNVREGGQSSPVILRIHELNSPVLFRNLDFFALFEN
DKDALGDEYIKRYEYQIQPGDVVHETLVLDPATRAIGFSVAFRDINGSSWRKVELIEEKSEYFLKLNLKESELSSNNTRG
IEQIYF*
MRKLLTFSLIFLLSGCTMWDQFKESTGITPETSSIELIITADKVLNVREGGQSSPVILRIHELNSPVLFRNLDFFALFEN
DKDALGDEYIKRYEYQIQPGDVVHETLVLDPATRAIGFSVAFRDINGSSWRKVELIEEKSEYFLKLNLKESELSSNNTRG
IEQIYF*
Nucleotide sequence: 501 bp
>T6CP259573 NZ_SRJG01000005:345059-345559 [Aliivibrio fischeri] [TssJ]
ATGCGAAAATTACTCACGTTTTCTCTTATTTTTTTATTATCTGGCTGCACTATGTGGGATCAGTTTAAAGAATCAACAGG
AATTACCCCTGAAACCTCTTCTATCGAGTTAATTATTACAGCAGATAAAGTATTAAATGTTCGTGAAGGTGGGCAATCAT
CCCCCGTCATATTGCGGATTCATGAACTAAACTCCCCTGTTCTTTTTCGTAACCTTGATTTCTTTGCGTTATTTGAAAAT
GACAAAGATGCACTTGGTGATGAATATATAAAACGATACGAATACCAAATTCAGCCTGGGGATGTAGTACATGAGACTCT
GGTATTAGACCCAGCGACTCGAGCTATCGGTTTCTCTGTAGCATTTAGAGACATAAACGGATCATCTTGGCGTAAAGTCG
AGTTAATCGAAGAAAAAAGCGAATACTTCTTAAAGCTAAACCTGAAAGAAAGTGAACTTAGCTCCAACAATACTCGCGGT
ATTGAGCAAATATATTTTTAA
ATGCGAAAATTACTCACGTTTTCTCTTATTTTTTTATTATCTGGCTGCACTATGTGGGATCAGTTTAAAGAATCAACAGG
AATTACCCCTGAAACCTCTTCTATCGAGTTAATTATTACAGCAGATAAAGTATTAAATGTTCGTGAAGGTGGGCAATCAT
CCCCCGTCATATTGCGGATTCATGAACTAAACTCCCCTGTTCTTTTTCGTAACCTTGATTTCTTTGCGTTATTTGAAAAT
GACAAAGATGCACTTGGTGATGAATATATAAAACGATACGAATACCAAATTCAGCCTGGGGATGTAGTACATGAGACTCT
GGTATTAGACCCAGCGACTCGAGCTATCGGTTTCTCTGTAGCATTTAGAGACATAAACGGATCATCTTGGCGTAAAGTCG
AGTTAATCGAAGAAAAAAGCGAATACTTCTTAAAGCTAAACCTGAAAGAAAGTGAACTTAGCTCCAACAATACTCGCGGT
ATTGAGCAAATATATTTTTAA