SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259570
Component protein type	TssJ
T6SS ID (Type)	T6SS17505 (Type i3)
Strain	Serratia marcescens strain KZ11
Replicon	chromosome
Sequence	Protein sequence (177 a.a.); Nucleotide sequence (531 bp)
Reference	[1] Steele MI, Motta EVS, Gattu T, et al. The Gut Microbiota Protects Bees from Invasion by a Bacterial Pathogen. Microbiol Spectr. 2021 Oct 31;9(2):e0039421. doi: 10.1128/Spectrum.00394-21. Epub 2021 Sep 15. PMID: 34523998

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C3R39_RS14000
Coordinate (Strand)	608618..609148 (-)
NCBI ID	WP_042705419.1
RefSeq	NZ_PQGJ01000003
Uniprot ID	A0A7I0PT42_SERMA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.7e-28	36..149

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MMTTPI
Transmembrane helix	7-29	ASRWCGMLFLLLGALALGGCMSS
Outside	30-176	AKSVPSRYSLVFDADRQVNAAAGAQPAPIKIRVLLLRSDAEFMDADFFSLQNDAKSVLGNSLLDSDQFFLTPGQTGKTLGGQSALDARYIGVIAEYQNLDGKTWRISLPLPEPTETNFYKVWQFSPDELEAHIVAGVNGLRPVKKVD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9871	pos: 25-26. LGG-CM.	MMTTPIASRWCGMLFLLLGALALGG

Protein sequence: 177 a.a.

						>T6CP259570 NZ_PQGJ01000003:c609148-608618 [Serratia marcescens] [TssJ]
MMTTPIASRWCGMLFLLLGALALGGCMSSAKSVPSRYSLVFDADRQVNAAAGAQPAPIKIRVLLLRSDAEFMDADFFSLQ
NDAKSVLGNSLLDSDQFFLTPGQTGKTLGGQSALDARYIGVIAEYQNLDGKTWRISLPLPEPTETNFYKVWQFSPDELEA
HIVAGVNGLRPVKKVD*					

Nucleotide sequence: 531 bp

						>T6CP259570 NZ_PQGJ01000003:c609148-608618 [Serratia marcescens] [TssJ]
ATGATGACGACCCCTATCGCTTCCCGCTGGTGCGGGATGCTGTTTCTGCTGCTGGGCGCGTTGGCGCTCGGCGGTTGCAT
GTCTTCCGCCAAAAGCGTGCCGTCGCGCTACAGCCTGGTGTTCGATGCCGATCGCCAGGTCAACGCCGCCGCCGGCGCGC
AACCGGCGCCGATAAAAATTCGCGTGCTGCTGCTGCGTTCGGATGCGGAATTCATGGATGCCGACTTCTTCAGTCTGCAA
AACGACGCCAAAAGCGTGCTCGGCAATAGCCTGCTGGACAGCGATCAGTTCTTCCTGACGCCGGGCCAGACCGGCAAGAC
GCTCGGCGGGCAAAGCGCGTTGGACGCACGTTACATCGGCGTCATCGCCGAGTATCAAAACCTGGACGGCAAGACCTGGC
GCATTTCGCTGCCGCTGCCGGAGCCCACCGAAACCAATTTCTACAAGGTATGGCAATTCTCGCCGGATGAGCTGGAAGCG
CATATCGTCGCGGGCGTGAATGGCCTGCGCCCCGTAAAAAAGGTCGACTGA					