SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP259549
Component protein type	TssJ
T6SS ID (Type)	T6SS17504 (Type i3)
Strain	Serratia marcescens strain KZ11
Replicon	chromosome
Sequence	Protein sequence (169 a.a.); Nucleotide sequence (507 bp)
Reference	[1] Steele MI, Motta EVS, Gattu T, et al. The Gut Microbiota Protects Bees from Invasion by a Bacterial Pathogen. Microbiol Spectr. 2021 Oct 31;9(2):e0039421. doi: 10.1128/Spectrum.00394-21. Epub 2021 Sep 15. PMID: 34523998

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C3R39_RS13360
Coordinate (Strand)	472158..472664 (+)
NCBI ID	WP_033632659.1
RefSeq	NZ_PQGJ01000003
Uniprot ID	A0A0P0QGX2_SERMA
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	8.5e-10	40..153

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-168	MVKTLRGMATGGLLAVGMLGGCSWFSHDEAVDAGRRLRFIDVEVTANDNVNPNAAGEAQPVKVCVIETNRSGWMPEGIGDGAPCRETAPSEGALSRHQSILQPRETLRYRFEVPAGQERWVVVGAEFQQISGVLPLAEQKSPAQADSQMKVMVGMTSLSIVTNDKMDE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9977	pos: 21-22. LGG-CS.	MVKTLRGMATGGLLAVGMLGG

Protein sequence: 169 a.a.

						>T6CP259549 NZ_PQGJ01000003:472158-472664 [Serratia marcescens] [TssJ]
MVKTLRGMATGGLLAVGMLGGCSWFSHDEAVDAGRRLRFIDVEVTANDNVNPNAAGEAQPVKVCVIETNRSGWMPEGIGD
GAPCRETAPSEGALSRHQSILQPRETLRYRFEVPAGQERWVVVGAEFQQISGVLPLAEQKSPAQADSQMKVMVGMTSLSI
VTNDKMDE*					

Nucleotide sequence: 507 bp

						>T6CP259549 NZ_PQGJ01000003:472158-472664 [Serratia marcescens] [TssJ]
ATGGTGAAAACGCTCCGGGGGATGGCGACAGGGGGACTGTTGGCGGTGGGGATGCTCGGCGGATGCAGCTGGTTTTCTCA
CGATGAGGCTGTGGATGCGGGGCGCCGGTTGCGGTTTATCGACGTCGAGGTGACCGCCAACGACAACGTAAACCCCAATG
CCGCCGGGGAGGCGCAGCCGGTCAAGGTGTGCGTGATAGAAACCAATCGCTCGGGGTGGATGCCCGAGGGGATTGGCGAC
GGCGCTCCTTGCCGCGAGACGGCGCCGTCGGAAGGGGCGCTGAGCCGGCACCAGAGCATTTTGCAACCGAGGGAGACGTT
GCGATATCGCTTCGAGGTGCCGGCGGGGCAAGAGCGATGGGTCGTCGTCGGGGCGGAGTTTCAGCAAATCAGCGGCGTGT
TGCCGCTCGCCGAGCAAAAGTCCCCTGCGCAGGCTGATTCGCAAATGAAGGTGATGGTGGGGATGACGTCACTCAGCATA
GTGACTAACGACAAAATGGATGAATGA					