SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP210297
Component protein type	TssJ
T6SS ID (Type)	T6SS14098 (Type i2)
Strain	Enterobacter cloacae strain DSM 30054
Replicon	chromosome
Sequence	Protein sequence (182 a.a.); Nucleotide sequence (546 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ16_RS21160
Coordinate (Strand)	4329260..4329805 (+)
NCBI ID	WP_013096443.1
RefSeq	NZ_CP056776
Uniprot ID	A0A0H3CI96_ENTCC
KEGG ID	enc:ECL_01819
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.7e-29	48..170

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-181	VLRTSLIKPACWLPALLAFSLTGCGITQGITDGTKSAFNAVFYKKIKVLHLDFTAREALNTDSRESNSLSEPVVVRVYQLKDRKIFDKTVYQQLLKDGETILKADLLTSRDVVVKPGGDVSLDMPMEEEAHFVAVVGLFRHPDMVNNTWKQVLRREDLDPDKPRILEAGNNHLTLQPRKDD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9982	pos: 23-24. LTG-CG.	VLRTSLIKPACWLPALLAFSLTG

Protein sequence: 182 a.a.

						>T6CP210297 NZ_CP056776:4329260-4329805 [Enterobacter cloacae] [TssJ]
VLRTSLIKPACWLPALLAFSLTGCGITQGITDGTKSAFNAVFYKKIKVLHLDFTAREALNTDSRESNSLSEPVVVRVYQL
KDRKIFDKTVYQQLLKDGETILKADLLTSRDVVVKPGGDVSLDMPMEEEAHFVAVVGLFRHPDMVNNTWKQVLRREDLDP
DKPRILEAGNNHLTLQPRKDD*					

Nucleotide sequence: 546 bp

						>T6CP210297 NZ_CP056776:4329260-4329805 [Enterobacter cloacae] [TssJ]
GTGTTACGTACAAGTTTGATTAAACCCGCGTGCTGGCTGCCTGCACTGCTGGCGTTCAGCCTCACGGGCTGTGGGATTAC
GCAGGGTATCACTGATGGCACTAAATCGGCATTTAACGCTGTGTTTTATAAAAAAATTAAGGTACTGCATCTGGATTTCA
CCGCCCGTGAAGCGTTGAACACCGATTCCCGCGAAAGTAACTCGCTGTCTGAGCCGGTGGTAGTCCGCGTATATCAACTG
AAAGACCGCAAGATCTTCGACAAAACGGTCTATCAGCAGCTGCTGAAGGACGGGGAGACCATTCTTAAAGCGGACCTTCT
GACCAGCCGTGACGTGGTGGTGAAGCCGGGCGGAGATGTGAGCCTTGATATGCCGATGGAAGAAGAAGCGCATTTTGTCG
CGGTGGTCGGGCTGTTCCGCCATCCGGATATGGTTAACAACACCTGGAAACAGGTGCTCAGACGTGAAGATCTCGATCCG
GATAAACCGCGCATTCTTGAAGCCGGGAATAACCATCTGACATTACAACCGCGAAAGGATGACTGA					