Detailed information of component protein


Summary

Component ID T6CP210294
Component protein type TssM
T6SS ID (Type) T6SS14098 experimental (Type i2)
Strain Enterobacter cloacae strain DSM 30054
Replicon chromosome
Sequence Protein sequence (1158 a.a.); Nucleotide sequence (3474 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) HWQ16_RS21130
Coordinate (Strand) 4320443..4323916 (+)
NCBI ID WP_013096438.1
RefSeq NZ_CP056776
Uniprot ID A0A0H3CHJ4_ENTCC
KEGG ID enc:ECL_01813
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 3.1e-61 432..749
IcmF_C PF06744.14 6.9e-21 1008..1112
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-12 VKLPFAFAPKTT
Transmembrane helix 13-35 EGRYLAVTLIFFLLLAAMTFMVW
Outside 36-49 KHPDIAGIQYGSQQ
Transmembrane helix 50-72 QTYWLIAGGCISGCTLIMLVVHL
Inside 73-383 WAALSAGKKEFDALVANVSGDDEKKKRENDNDPVHPAVVMCDRIRAHLRSRISLYWRSKTRLLLITGDEAAIEQLVPGLQQQQWLEGNRTVLIYGGSLASEPDREKYIALRKLRRGRPLDGIIRVLPQSLKLTPQISDNDLRGLEKISELLRYSAPVWLWQLCGSNWSQDTRPEQAVGASFPLRANEGDVIRQLERMLPALRTQGMSQVSENNRHDFLLRLGQHLQDGGIVRWTEQLVPWLAASRQRVPLRGLMFSLPEKKTAGTSASTADAEQYIPGSQRHALTLPAIWQGTVDDCPRVRGRRVGMAWEQ
Transmembrane helix 384-406 TLAWTLMAIIGVWGAGTLLSFAV
Outside 407-1157 NRSQIVSVAQQAHALVEHPSVSDYQLTALHTLRNDAGRLQHRIQDGAPWYQRFGLDHNQQLLDAMLPWYGVANNRLIRDPANEALKQKLSALANYAPNSDQRARLAKPGYDQLKAWLMMARPDKADGAFYAQTMKTVQPTRMGISAGLWQSLSPDLWAFYISELPAQPQWKITPDAQLVSQGRQVLLQQIGRRNAESTLYENMLKSVRRNFADVSLEDMTGGTDARRLFTTDEVVPGMFTRQAWEGGIQQAIEKAANSRRDEIDWVLSDSRKAVSSDLSPEALKARLTQRYFTDFAGSWLSFLNSLQLNPANNIADVTDQLTLMSDVRQSPLIALMNTLAWQGQTGQQREALSDSIIKSAKDLVGGKDKPAIDQSASGPQGPLDDTFGPLLQLLGKNKGSNVMSADNSLSLQTYLTRITRVRLRLQQVASASDPQAMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGFGSTMFVQPLTQAWETVLQPSAASLNDKWSRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELGGVLHKEGSQWVPDKVNSQGLSFNPSFLRAINQLSQLSDILFTDGSQGISFELQARPVPQVVETQLTVDGQKLHYFNQMADWQSFRWPGDTYKPGTMLTWTTVNAGARLFRDYSGTWGFIRWLDQGKCAQLDRSQWMMSFTAPDGRTLQWVLRSQLGSGPLALLALRGFTLPDQIFSVDSAATAQALMDSTESSDMDGTE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9919 - -


  Protein sequence: 1158 a.a.    

>T6CP210294 NZ_CP056776:4320443-4323916 [Enterobacter cloacae] [TssM]
VKLPFAFAPKTTEGRYLAVTLIFFLLLAAMTFMVWKHPDIAGIQYGSQQQTYWLIAGGCISGCTLIMLVVHLWAALSAGK
KEFDALVANVSGDDEKKKRENDNDPVHPAVVMCDRIRAHLRSRISLYWRSKTRLLLITGDEAAIEQLVPGLQQQQWLEGN
RTVLIYGGSLASEPDREKYIALRKLRRGRPLDGIIRVLPQSLKLTPQISDNDLRGLEKISELLRYSAPVWLWQLCGSNWS
QDTRPEQAVGASFPLRANEGDVIRQLERMLPALRTQGMSQVSENNRHDFLLRLGQHLQDGGIVRWTEQLVPWLAASRQRV
PLRGLMFSLPEKKTAGTSASTADAEQYIPGSQRHALTLPAIWQGTVDDCPRVRGRRVGMAWEQTLAWTLMAIIGVWGAGT
LLSFAVNRSQIVSVAQQAHALVEHPSVSDYQLTALHTLRNDAGRLQHRIQDGAPWYQRFGLDHNQQLLDAMLPWYGVANN
RLIRDPANEALKQKLSALANYAPNSDQRARLAKPGYDQLKAWLMMARPDKADGAFYAQTMKTVQPTRMGISAGLWQSLSP
DLWAFYISELPAQPQWKITPDAQLVSQGRQVLLQQIGRRNAESTLYENMLKSVRRNFADVSLEDMTGGTDARRLFTTDEV
VPGMFTRQAWEGGIQQAIEKAANSRRDEIDWVLSDSRKAVSSDLSPEALKARLTQRYFTDFAGSWLSFLNSLQLNPANNI
ADVTDQLTLMSDVRQSPLIALMNTLAWQGQTGQQREALSDSIIKSAKDLVGGKDKPAIDQSASGPQGPLDDTFGPLLQLL
GKNKGSNVMSADNSLSLQTYLTRITRVRLRLQQVASASDPQAMMQTLAQTVFQGKSVDLTDTQQYGSLISASLGEEWSGF
GSTMFVQPLTQAWETVLQPSAASLNDKWSRSVVANWHTAFDGRFPFAASKSDASLPMLAEFVRKDSGRIERFLTTELGGV
LHKEGSQWVPDKVNSQGLSFNPSFLRAINQLSQLSDILFTDGSQGISFELQARPVPQVVETQLTVDGQKLHYFNQMADWQ
SFRWPGDTYKPGTMLTWTTVNAGARLFRDYSGTWGFIRWLDQGKCAQLDRSQWMMSFTAPDGRTLQWVLRSQLGSGPLAL
LALRGFTLPDQIFSVDSAATAQALMDSTESSDMDGTE*

  Nucleotide sequence: 3474 bp    

>T6CP210294 NZ_CP056776:4320443-4323916 [Enterobacter cloacae] [TssM]
GTGAAGCTACCTTTTGCTTTTGCACCGAAGACTACGGAAGGCCGCTACCTTGCTGTCACATTGATTTTCTTTTTGTTGTT
AGCCGCTATGACGTTCATGGTCTGGAAGCATCCGGATATTGCCGGGATTCAGTACGGTAGTCAGCAGCAAACTTACTGGC
TTATCGCTGGCGGATGTATTTCAGGATGCACACTTATCATGCTGGTTGTGCACCTGTGGGCTGCTCTCAGTGCCGGTAAA
AAGGAGTTTGATGCGCTTGTCGCCAATGTCAGTGGTGATGATGAGAAAAAGAAACGGGAAAATGATAACGACCCTGTTCA
CCCTGCGGTGGTCATGTGTGACAGGATTCGGGCGCATCTCCGCTCCCGTATTAGTCTTTACTGGCGCAGCAAAACCCGTC
TGCTGTTAATCACTGGCGATGAGGCGGCCATCGAACAACTGGTACCCGGCCTGCAACAGCAGCAGTGGCTTGAAGGTAAC
CGCACCGTGCTGATTTACGGCGGCAGCCTGGCATCGGAGCCCGACCGCGAAAAATACATCGCGCTGCGCAAGCTGCGACG
CGGACGCCCGCTGGACGGGATTATCCGTGTACTACCGCAGTCGCTTAAGCTGACCCCGCAAATCAGCGACAACGATTTAC
GCGGGCTGGAAAAAATCAGCGAACTGCTGCGCTATTCCGCCCCGGTCTGGCTGTGGCAGTTGTGTGGCAGCAACTGGTCG
CAGGATACGCGTCCTGAGCAGGCGGTAGGCGCAAGCTTTCCTCTCCGCGCAAACGAAGGCGACGTGATCCGCCAACTCGA
GCGGATGCTGCCGGCGCTGCGGACGCAGGGGATGAGTCAGGTCTCCGAAAACAACCGCCACGACTTCCTGTTGCGCCTGG
GCCAGCATCTCCAGGACGGCGGTATCGTCCGCTGGACTGAGCAACTGGTGCCATGGTTGGCTGCCTCCCGGCAGCGCGTT
CCCCTGCGGGGGCTGATGTTCAGCCTGCCGGAGAAGAAAACTGCCGGTACGTCAGCGAGCACAGCCGACGCTGAGCAATA
TATTCCGGGGTCACAGCGTCATGCACTGACCCTGCCGGCAATCTGGCAGGGTACCGTTGACGACTGCCCCCGCGTTCGTG
GCCGCCGTGTCGGTATGGCGTGGGAGCAGACGCTTGCCTGGACGCTGATGGCCATTATCGGTGTCTGGGGGGCGGGAACG
CTGCTGTCGTTCGCGGTTAACCGGTCACAGATAGTCTCTGTTGCGCAGCAGGCGCATGCACTGGTGGAGCACCCTTCCGT
ATCGGATTACCAGCTGACGGCCCTGCATACCCTGCGCAATGATGCCGGCCGCCTGCAGCACCGTATCCAGGACGGAGCGC
CCTGGTATCAGCGCTTCGGCCTCGACCATAATCAGCAGCTGCTTGATGCGATGCTGCCCTGGTACGGCGTGGCAAACAAC
CGCCTGATACGCGACCCGGCAAATGAGGCGCTTAAGCAGAAGCTCAGCGCGCTGGCAAATTATGCCCCCAACAGTGACCA
ACGGGCACGACTGGCGAAACCGGGCTATGACCAGCTGAAAGCCTGGCTGATGATGGCTCGTCCGGATAAAGCCGACGGCG
CATTCTACGCGCAGACCATGAAAACCGTGCAGCCGACGCGAATGGGTATCTCAGCAGGCCTGTGGCAAAGCCTGTCACCG
GATTTGTGGGCTTTTTATATATCCGAACTGCCAGCGCAGCCACAGTGGAAAATCACGCCGGATGCGCAGTTGGTCAGCCA
GGGCCGCCAGGTGCTGCTGCAGCAGATAGGACGGCGCAACGCGGAAAGCACGCTGTACGAGAACATGCTCAAATCCGTGC
GCCGTAACTTTGCCGATGTGTCTCTGGAGGACATGACTGGCGGTACCGATGCCCGTCGCCTGTTTACCACCGATGAGGTG
GTACCGGGAATGTTCACCCGTCAGGCCTGGGAGGGGGGGATTCAGCAGGCTATCGAAAAGGCCGCAAATTCCCGCCGCGA
TGAAATCGACTGGGTACTGAGCGACAGCCGAAAAGCCGTCTCCTCAGACCTGTCGCCGGAAGCACTGAAAGCGCGTCTGA
CACAACGCTACTTCACTGACTTTGCTGGCAGCTGGCTGAGCTTCCTCAACAGCCTGCAACTTAATCCGGCGAACAACATA
GCGGATGTCACAGACCAACTGACGCTGATGAGCGATGTCCGCCAGTCACCGCTGATCGCCTTGATGAATACCCTCGCCTG
GCAGGGACAGACCGGACAGCAGCGCGAAGCTCTTTCGGACTCCATCATCAAATCGGCTAAAGATCTCGTAGGTGGAAAAG
ATAAGCCTGCGATTGATCAGTCTGCGTCAGGACCGCAGGGGCCGCTGGATGACACCTTTGGACCGTTGCTTCAGCTTCTG
GGTAAAAACAAGGGCAGCAACGTCATGTCGGCTGATAACTCCCTGAGCCTGCAGACGTACCTGACCCGTATCACCCGCGT
GCGTCTACGCCTGCAACAGGTGGCTAGCGCCTCTGACCCGCAGGCGATGATGCAGACCCTGGCGCAGACCGTATTCCAGG
GCAAAAGCGTGGACCTGACCGACACCCAGCAATACGGCAGTCTCATTTCTGCAAGTCTTGGCGAAGAGTGGAGTGGTTTT
GGCAGCACGATGTTTGTGCAGCCTCTGACCCAGGCATGGGAAACCGTGCTTCAGCCGTCGGCAGCGAGCCTCAATGACAA
GTGGAGCCGCTCTGTGGTGGCGAACTGGCACACCGCATTTGACGGTCGTTTCCCGTTTGCCGCGAGTAAAAGTGATGCCT
CCCTGCCGATGCTGGCTGAATTTGTGCGTAAGGACAGCGGACGTATTGAACGCTTCCTGACGACAGAGCTCGGTGGTGTT
CTGCACAAGGAGGGCAGCCAGTGGGTGCCGGATAAAGTCAACAGCCAGGGGCTGAGTTTTAACCCGTCCTTCCTGCGGGC
GATTAATCAGTTGAGTCAGTTGTCGGACATTTTGTTTACCGACGGCAGCCAGGGTATCAGCTTCGAACTGCAGGCGCGTC
CGGTACCGCAGGTGGTGGAGACGCAACTGACTGTTGATGGACAAAAGCTGCACTACTTTAACCAGATGGCTGACTGGCAG
TCCTTCCGCTGGCCGGGAGACACGTACAAGCCGGGAACCATGCTGACCTGGACCACGGTCAACGCGGGTGCGCGTCTGTT
CAGGGATTACAGCGGAACCTGGGGCTTTATTCGCTGGCTGGACCAGGGCAAGTGTGCGCAGCTTGATCGCAGTCAGTGGA
TGATGAGTTTCACCGCGCCGGATGGCCGTACCCTGCAGTGGGTGCTGCGTTCACAGCTGGGCAGCGGGCCGCTGGCGTTA
CTGGCACTGCGCGGCTTCACGCTGCCGGATCAGATATTCAGCGTCGACAGCGCGGCCACGGCGCAGGCGCTGATGGACAG
TACGGAAAGCAGTGATATGGACGGAACGGAATAA