SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP210278
Component protein type	TssE
T6SS ID (Type)	T6SS14097 (Type i3)
Strain	Enterobacter cloacae strain DSM 30054
Replicon	chromosome
Sequence	Protein sequence (188 a.a.); Nucleotide sequence (564 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ16_RS19775
Coordinate (Strand)	4054237..4054800 (+)
NCBI ID	WP_013096188.1
RefSeq	NZ_CP056776
Uniprot ID	A0A421IID8_ENTCL, A0A331GPH6_KLEPN, A0A0H3CIY4_ENTCC
KEGG ID	enc:ECL_01548, eclg:EC036_25950
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	1.9e-21	59..163

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-187	MSNPAGEGDLLRSGWQARSKQEKVGARDKMQPSLLDRLTDNDPEKKRESANSNLITHAALRRNVLRDLQWLFNTINHDASGDLSMLPHVSRSVVNFGVAPLAGKRMSDIEWHDIQRKLTEAIVNFEPRILPQGLQVRCVSDTSSLDLHNVLSIEIKGRLWCVPYPLEFLFRTDVDLENGHFELKDAG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9875	-	-

Protein sequence: 188 a.a.

						>T6CP210278 NZ_CP056776:4054237-4054800 [Enterobacter cloacae] [TssE]
MSNPAGEGDLLRSGWQARSKQEKVGARDKMQPSLLDRLTDNDPEKKRESANSNLITHAALRRNVLRDLQWLFNTINHDAS
GDLSMLPHVSRSVVNFGVAPLAGKRMSDIEWHDIQRKLTEAIVNFEPRILPQGLQVRCVSDTSSLDLHNVLSIEIKGRLW
CVPYPLEFLFRTDVDLENGHFELKDAG*					

Nucleotide sequence: 564 bp

						>T6CP210278 NZ_CP056776:4054237-4054800 [Enterobacter cloacae] [TssE]
ATGAGTAATCCTGCTGGCGAAGGCGATCTGCTGCGCAGCGGCTGGCAAGCCCGCAGTAAGCAGGAGAAGGTGGGGGCGCG
CGATAAAATGCAGCCCTCGCTGCTCGATCGCCTCACCGATAACGACCCGGAGAAAAAGCGCGAGTCGGCCAACAGCAACC
TGATCACCCATGCCGCGCTGCGTCGCAACGTGCTGCGGGATCTGCAGTGGCTGTTTAATACCATCAATCACGACGCCTCC
GGCGATTTGAGCATGCTGCCACACGTAAGCCGTTCGGTAGTGAATTTTGGCGTTGCGCCGCTGGCCGGAAAGCGCATGTC
GGATATCGAATGGCACGACATTCAGCGCAAACTCACCGAGGCCATTGTCAACTTTGAACCCCGCATTTTGCCCCAGGGGC
TGCAGGTCCGCTGTGTGTCGGACACCTCCTCGCTGGATCTGCACAACGTGCTCTCCATCGAGATAAAAGGGCGTTTGTGG
TGTGTGCCGTACCCGCTGGAGTTTCTGTTTCGTACCGATGTCGATCTGGAAAACGGCCACTTTGAACTGAAAGATGCGGG
GTGA					