SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP210266
Component protein type	TssJ
T6SS ID (Type)	T6SS14097 (Type i3)
Strain	Enterobacter cloacae strain DSM 30054
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ16_RS19700
Coordinate (Strand)	4037435..4037938 (+)
NCBI ID	WP_013096175.1
RefSeq	NZ_CP056776
Uniprot ID	A0A5E1AD78_9ENTR, A0A1B3F244_ENTCL, A0A331GQD1_KLEPN, A0A0H3CIW8_ENTCC
KEGG ID	enc:ECL_01533, eclg:EC036_26100
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	7.5e-25	34..137

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-9	MNNKNFHKL
Transmembrane helix	10-29	WFVFYAVIFALVSGCSSSSH
Inside	30-167	SDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATLGANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQMLDGKKWRVSLPLPVPGENHLYQFWKWSSDELQANVFLDINGIRVISQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9981	pos: 23-24. VSG-CS.	MNNKNFHKLWFVFYAVIFALVSG

Protein sequence: 168 a.a.

						>T6CP210266 NZ_CP056776:4037435-4037938 [Enterobacter cloacae] [TssJ]
MNNKNFHKLWFVFYAVIFALVSGCSSSSHSDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATL
GANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQMLDGKKWRVSLPLPVPGENHLYQFWKWSSDELQANVFLDIN
GIRVISQ*					

Nucleotide sequence: 504 bp

						>T6CP210266 NZ_CP056776:4037435-4037938 [Enterobacter cloacae] [TssJ]
ATGAATAATAAAAATTTTCATAAGCTGTGGTTCGTCTTTTACGCGGTGATATTCGCCCTGGTCAGTGGTTGTTCGTCGTC
TTCACACAGCGACCCCTCCCGCTACAATCTGCAGTTTCAGGCTCATCCACAAATCAATGATTCTGCGCCGCTTAAAGTCA
GAGTGCTGCTGTTGAAGTCCGATGCGGATTTCATGTCCAGTGACTTCTATTCCCTGCAGAACAACGCGTCAGCCACGCTT
GGCGCGAATCTGCTGAACAGCGATGTTTTCTTCCTGATGCCGGGCCAGCTTTCGAAAACCCTTAGCGGACAAAGCTCACC
CGAGGCGCGCTATATCGGTGTTATGGCCGAATATCAGATGCTGGATGGAAAGAAATGGCGTGTCTCACTCCCACTGCCTG
TTCCGGGCGAAAACCATCTCTACCAGTTCTGGAAATGGTCATCCGATGAACTCCAGGCCAATGTCTTTCTCGACATAAAT
GGCATCCGGGTCATCAGCCAGTAA					