SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP207896
Component protein type	TssJ
T6SS ID (Type)	T6SS13923 (Type i3)
Strain	Enterobacter sp. DSM 30060
Replicon	chromosome
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ15_RS21430
Coordinate (Strand)	4496293..4496796 (-)
NCBI ID	WP_022648418.1
RefSeq	NZ_CP056118
Uniprot ID	A0A179PEB8_9ENTR, A0A7H9JVA5_ENTCL
KEGG ID	ecli:ECNIH5_12545
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	4.5e-25	34..136

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MNNKNF
Transmembrane helix	7-29	HRMWFPFFALILALIGGCTSSSH
Outside	30-167	SDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATLGANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQALDGKKWRVSLPLPVPGENAIYQFWKWSADELQASVFLDVNGIRVISQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9977	pos: 23-24. IGG-CT.	MNNKNFHRMWFPFFALILALIGG

Protein sequence: 168 a.a.

						>T6CP207896 NZ_CP056118:c4496796-4496293 [Enterobacter sp. DSM 30060] [TssJ]
MNNKNFHRMWFPFFALILALIGGCTSSSHSDPSRYNLQFQAHPQINDSAPLKVRVLLLKSDADFMSSDFYSLQNNASATL
GANLLNSDVFFLMPGQLSKTLSGQSSPEARYIGVMAEYQALDGKKWRVSLPLPVPGENAIYQFWKWSADELQASVFLDVN
GIRVISQ*					

Nucleotide sequence: 504 bp

						>T6CP207896 NZ_CP056118:c4496796-4496293 [Enterobacter sp. DSM 30060] [TssJ]
ATGAATAATAAAAATTTCCATCGGATGTGGTTCCCTTTTTTTGCGTTGATTCTCGCGCTGATCGGTGGTTGTACGTCGTC
TTCACACAGCGACCCCTCCCGCTACAATCTTCAGTTTCAGGCTCATCCTCAAATCAATGATTCTGCACCGCTTAAGGTCA
GAGTATTGCTGCTGAAATCCGATGCGGATTTCATGTCCAGCGACTTCTACTCCTTACAGAACAACGCGTCAGCCACGCTT
GGCGCGAATCTGCTGAACAGCGATGTGTTCTTCCTGATGCCGGGCCAGCTTTCGAAAACCCTGAGCGGTCAAAGCTCACC
GGAAGCGCGCTATATCGGCGTAATGGCGGAATACCAGGCACTGGACGGCAAAAAATGGCGCGTCTCACTTCCTTTGCCTG
TTCCCGGCGAAAACGCGATTTACCAGTTCTGGAAATGGTCCGCAGATGAACTCCAGGCCAGCGTCTTCCTTGACGTAAAT
GGTATCCGGGTCATCAGCCAGTAA					