SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP207891
Component protein type	TssA
T6SS ID (Type)	T6SS13923 (Type i3)
Strain	Enterobacter sp. DSM 30060
Replicon	chromosome
Sequence	Protein sequence (339 a.a.); Nucleotide sequence (1017 bp)
Reference	[1] Flaugnatti N, Isaac S, Lemos Rocha LF, et al. Human commensal gut Proteobacteria withstand type VI secretion attacks through immunity protein-independent mechanisms. Nat Commun. 2021 Oct 1;12(1):5751. doi: 10.1038/s41467-021-26041-0. PMID: 34599171

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HWQ15_RS21405
Coordinate (Strand)	4488281..4489297 (-)
NCBI ID	WP_023300332.1
RefSeq	NZ_CP056118
Uniprot ID	A0A155VAP1_9ENTR
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.5e-37	7..130

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-338	MNIDEFLAPISPDNPCGENLEYDADFQAMGQASQGKAEQQFGDTIIPAEPADWNTVEKLATSLLGRTKDLRVMLALTHAWTRRRGLAGYADGLLLVQEALSRYWEQLYPLLEEYGETDPFYRINALAGLSDKSDLTVAVRNASLLRSNGDEISLRDAQALLDGSKTECPDYPGGRPRLIDELARGDQPGTEAVIVINERLLAIRELLTGYLGESGVPEMEQLLKTVGQVSSACQVTDISKLLPNRDAQAEQHAEPQPVTASPVQQVTDWRSVQVTSRADAQLMLEKAKQYFAQYEPSHPAPLMIERVQRLSELNFMDIIRDLAPDGVNQLENIFGRRE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9832	-	-

Protein sequence: 339 a.a.

						>T6CP207891 NZ_CP056118:c4489297-4488281 [Enterobacter sp. DSM 30060] [TssA]
MNIDEFLAPISPDNPCGENLEYDADFQAMGQASQGKAEQQFGDTIIPAEPADWNTVEKLATSLLGRTKDLRVMLALTHAW
TRRRGLAGYADGLLLVQEALSRYWEQLYPLLEEYGETDPFYRINALAGLSDKSDLTVAVRNASLLRSNGDEISLRDAQAL
LDGSKTECPDYPGGRPRLIDELARGDQPGTEAVIVINERLLAIRELLTGYLGESGVPEMEQLLKTVGQVSSACQVTDISK
LLPNRDAQAEQHAEPQPVTASPVQQVTDWRSVQVTSRADAQLMLEKAKQYFAQYEPSHPAPLMIERVQRLSELNFMDIIR
DLAPDGVNQLENIFGRRE*					

Nucleotide sequence: 1017 bp

						>T6CP207891 NZ_CP056118:c4489297-4488281 [Enterobacter sp. DSM 30060] [TssA]
ATGAATATCGATGAATTTCTCGCGCCGATAAGCCCCGATAACCCCTGCGGTGAAAACCTGGAGTACGACGCTGATTTCCA
GGCCATGGGGCAGGCCAGTCAGGGCAAAGCCGAACAGCAGTTTGGCGACACCATCATTCCGGCAGAGCCTGCCGACTGGA
ACACGGTGGAAAAACTCGCCACCAGCCTGCTGGGCCGCACCAAAGATCTTCGCGTCATGCTGGCGTTAACCCATGCCTGG
ACGCGTCGTCGCGGGCTGGCAGGTTACGCCGACGGGCTACTGCTGGTGCAGGAAGCCCTGTCCCGTTACTGGGAGCAACT
TTACCCGCTGCTGGAAGAGTATGGCGAAACCGATCCGTTCTACCGCATTAACGCCCTCGCCGGGCTGAGCGATAAATCTG
ACCTGACGGTCGCGGTGCGTAACGCCTCACTGCTCCGCTCTAACGGCGATGAGATTTCGCTCCGTGACGCCCAGGCGCTG
CTTGATGGCAGTAAAACAGAGTGTCCGGATTATCCGGGCGGTCGTCCACGACTGATCGATGAGCTGGCCCGGGGCGATCA
GCCAGGCACCGAAGCGGTAATCGTGATCAACGAACGCCTGCTCGCCATCCGTGAACTGCTTACCGGCTATCTCGGGGAAA
GTGGCGTACCGGAGATGGAACAGCTGCTGAAAACCGTCGGGCAAGTTTCGAGCGCCTGTCAGGTGACCGATATCAGCAAG
TTGCTGCCGAACCGTGACGCGCAGGCTGAGCAGCACGCCGAGCCGCAGCCGGTGACCGCGTCGCCCGTTCAGCAGGTGAC
GGACTGGCGCAGCGTGCAGGTGACCAGCCGCGCCGACGCGCAGCTGATGCTGGAAAAAGCGAAACAGTATTTTGCGCAGT
ACGAACCCAGCCACCCCGCACCGCTGATGATTGAACGGGTGCAGCGGCTGTCTGAACTCAACTTTATGGACATTATTCGC
GACCTGGCGCCAGACGGCGTTAACCAACTGGAAAACATCTTTGGACGCCGCGAATGA					